Extracting method of duck chondroitin sulfate by compound enzymolysis

A technology of chondroitin sulfate and cartilage, which is applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of lack of systematic research on effective active substances and their functions of duck cartilage, and the technology of extracting chondroitin sulfate is blank, etc., to achieve shortening The effect of production cycle, reduction of production cost and increase of production efficiency

Active Publication Date: 2013-08-14
QINGDAO AGRI UNIV
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Problems solved by technology

[0006] Up to now, a lot of research has been carried out on the extraction methods of chondroitin sulfate from fish, pigs, cattle, sheep and other animals at home and abroad, but there is a lack of systematic research on the effective active substances and their functions in duck cartilage. The technology of extracting chondroitin sulfate is still blank

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  • Extracting method of duck chondroitin sulfate by compound enzymolysis
  • Extracting method of duck chondroitin sulfate by compound enzymolysis
  • Extracting method of duck chondroitin sulfate by compound enzymolysis

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preparation example Construction

[0024] The preparation method of the present invention comprises the steps of:

[0025] 1) Compound protease formula: collagenase, lipase, glycogenase, nuclease, according to the ratio of parts by weight: 1.5:1:0.5:0.5; and you can choose to add a certain amount of trypsin to increase the effect of enzymatic hydrolysis, add The amount is 50% of the total weight of collagenase, lipase, glycogenase and nuclease. During the enzymolysis process, the ratio of duck breast cartilage bone powder, novel composite protease and trypsin is 100:1:0.5 in parts by weight.

[0026] Since the main components of duck breast cartilage include chondroitin sulfate, type II collagen, fat and a small amount of nucleic acid substances; for this purpose, a new composite protease is designed, and its enzyme system mainly contains collagenase, lipase and glycogenase and nuclease, which can completely hydrolyze collagen, fat and nucleic acid substances in duck breast cartilage during the enzymatic extra...

Embodiment 1

[0037] Embodiment 1: the screening test of protease

[0038] Raw material processing: Place the duck breast cartilage in a cooking pot with a constant temperature of 60°C to 80°C for 0.5h to 1h, remove the excess meat, fascia and grease on the cartilage, and rinse it with clean water. Then soak in methanol: chloroform (1-3:1) at low temperature for 7h-9h to remove fat, and rinse with clean water. Then soak the aggregate with 0.5mol / L~1.5mol / L NaCl solution, and slowly stir to remove most of the salt-soluble impurities, so that the duck cartilage is white. After drying and crushing at 60-80 mesh, store at -20°C for later use.

[0039] ① Compound protease and single enzyme enzymatic hydrolysis test

[0040] Take a certain amount of protease (new compound protease, papain, trypsin, alkaline protease, neutral protease), put it into a beaker, add a certain amount of distilled water and shake well, then add a certain amount of pre-treated duck breast cartilage powder, Enzymolyze ...

Embodiment 2

[0046] Example 2: Screening of the best process for compound protease and trypsin compound proteolysis

[0047] (1) Enzymatic hydrolysis single factor test

[0048] Enzymolysis treatment: Weigh 10g of pre-treated duck breast cartilage bone powder into a 250mL beaker, adjust the pH value of the solution, add a certain concentration of new compound protease and trypsin according to a certain ratio of solid to liquid, and extract a certain amount in a constant temperature water bath. time, inactivate the enzyme in a boiling water bath for 10 minutes, and then filter out the enzymolysis solution with four layers of filter cloth.

[0049] ①Effect of material-water ratio on extraction of duck chondroitin sulfate

[0050] The test results show that the extraction rate of duck chondroitin sulfate enzymatic hydrolysis first increases and then decreases with the increase of material-water ratio, and reaches the maximum value at 1:10, but no matter the extent of increase or decrease is ...

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Abstract

The invention discloses an extracting method of duck chondroitin sulfate by compound enzymolysis. A basic formula of novel compound protease, which is used for extracting duck chondroitin sulfate, is designed according to chemical composition of the duck breast cartilage; and the compound enzyme is utilized to prepare duck chondroitin sulfate; the process comprises the following four steps of: preparing duck breast cartilage powder, extracting the duck breast cartilage powder by enzymolysis; settling protein; and preparing chondroitin sulfate powder. The extracting method can be used for extracting chondroitin sulfate of the duck breast cartilage by adopting a compound enzymolysis method; the novel compound protease and trypsin are compounded, so that an alkaline extraction step is omitted, and the use of the compound enzyme is optimized, and therefore, the yield is improved by about 20% compared with the yield achieved by adoption of other methods, and the product purity reaches 90%-95%. The compound enzyme can be used for substituting dilute alkali or thick alkali for decomposing the cartilage, so that the pollution to the environment is greatly lowered, the production period can be effectively shortened, the production cost is lowered, the product quality is improved, and the blank in the field is filled.

Description

technical field [0001] The invention belongs to the technical field of chondroitin sulfate preparation, and in particular relates to an extraction method of duck chondroitin sulfate compound enzymolysis. Background technique [0002] Chondroitin sulfate (CS) is an acidic mucopolysaccharide composed of repeating disaccharide units linked by D-glucuronic acid and N-acetylgalactosamine through β-1,4-glycosidic bonds. Chondroitin sulfate has various physiological functions such as anticoagulation, anti-inflammation, anti-tumor, anti-virus, anti-oxidation, lowering blood fat, preventing atherosclerosis, regulating water in the body, scavenging free radicals and delaying aging. It can be used in food, Medicines, various health care products and high-end cosmetics. [0003] my country is the country with the largest output of chondroitin sulfate in the world, accounting for more than 80% of the global output, with an average annual output of more than 4,000 tons; my country's chon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12N9/50C08B37/08C12N9/24C12N9/22C12N9/76
Inventor 王宝维陈晨葛文华张名爱岳斌
Owner QINGDAO AGRI UNIV
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