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A detection method and detection kit for thiazolidinone drugs

A technology of thiazolidinones and detection methods, which is applied in the measurement of color/spectral characteristics, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of expensive instruments and operation. Complex maintenance, high analysis costs, to reduce analysis costs, improve inspection efficiency, and reduce analysis costs

Active Publication Date: 2015-11-18
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: (1) The instrument is expensive, and the sample pretreatment time is long. Most of them are for a single product, and the mobile phase used is mostly a certain proportion of methanol and salt solution. Contaminated, high analysis cost; (2) When the chromatographic retention time of the coexisting components is close to that of thiazolidinones, it is easy to make a wrong judgment; (3) The instrument has high requirements for the use environment and needs to be fixed. Not suitable for on-site testing with high mobility
[0011] Disadvantages: The detection equipment is very expensive, and the operation and maintenance are complicated, and the detection cycle is long, especially not suitable for on-site detection

Method used

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  • A detection method and detection kit for thiazolidinone drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Detection of Avandia (tablets, the marked dosage is one tablet each time, and the results of high-performance liquid chromatography determine that each tablet of this product contains 4 mg of rosiglitazone)

[0029] Take a piece of content, grind it evenly, put it into a 10mL sample bottle, add a 98% methanol aqueous solution by volume, tighten the bottle cap, mix well and filter. Collect 1 mL of the filtrate, add 5 mL of chloroform, and mix with a vortex mixer for 1 minute for extraction. After the solution is separated, the chloroform layer is dried over anhydrous sodium sulfate. Add 1 mL of pH 3.5 phthalic acid buffer solution and 0.6 mL of 0.2% bromocresol green color development solution to the extract, and the solution immediately turns yellow, which indicates that the sample contains thiazolidinones.

[0030] Put the colored solution into the 420nm wavelength place to measure its absorbance. According to the rosiglitazone concentration-ultraviolet absor...

Embodiment 2

[0031] Example 2 Detection of Sennics (tablets, the marked dosage is once a day, 4 tablets each time, and the results of high performance liquid chromatography. Each tablet of this product contains 1 mg of pioglitazone)

[0032] Take 4 pieces of the sample, grind them evenly, place them in a 10mL sample bottle, add methanol aqueous solution with a concentration of 95% by volume, tighten the bottle cap, shake vigorously and mix well, let stand, filter, collect 1mL of the filtrate, add 5 mL of diethyl ether was mixed with a vortex mixer for 1 minute for extraction. After the solution was separated into layers, the diethyl ether layer was dried over anhydrous sodium sulfate. Add 1 mL of pH 3.0 phthalic acid buffer solution and 0.6 mL of 0.5% bromocresol green color development solution to the extract, and the solution immediately turns yellow, which indicates that the sample contains thiazolidinones.

[0033] For the above operations, centrifugation instead of filtration has the ...

Embodiment 3

[0034] Example 3 Detection of Tang Niao Le Capsules (capsules, the labeled dosage is 3 to 4 capsules each time, as determined by high performance liquid chromatography-mass spectrometry, this product does not contain thiazolidinones).

[0035] Take one of the contents, grind it evenly, put it into a 10mL sample bottle, add a 96% methanol aqueous solution by volume, tighten the bottle cap, mix well and filter. Collect 1 mL of the filtrate, add 5 mL of ethyl acetate, and mix with a vortex mixer for 1 minute for extraction. After the solution is separated, the ethyl acetate layer is dried over anhydrous sodium sulfate. Add 1 mL of pH 5.0 phthalic acid buffer solution and 0.6 mL of 0.3% bromocresol green color development solution to the extract, and the solution does not change color, so it can be judged that the sample does not contain thiazolidinones.

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Abstract

The invention discloses a quick detecting method of thiazolidone drug. The method comprises: extracting a sample through methanol, adding a buffering solution and a color developing agent after the extraction treatment, and judging the result through a chromogenic reaction. The invention further provides a special kit for the method of the invention. The method disclosed by the invention is low in analysis cost, free of special equipment, high in sensitivity, suitable for onsite inspection and capable of overcoming the defects of the prior art.

Description

technical field [0001] The invention relates to a detection method and a detection kit for thiazolidinone drugs. Background technique [0002] Thiazolidinones not only reduce microvascular complications of diabetes due to their good hypoglycemic effect, but also are expected to greatly reduce macrovascular complications of diabetes due to their beneficial effects on hypertension and hyperlipidemia. [0003] However, thiazolidinones have the risk of causing cardiovascular disease, and taking them without a doctor's advice may lead to serious adverse drug reactions and even death. Patients need to take it under the guidance of a doctor, and the country has strict regulations on its production, operation and use. [0004] It is a long-term task to investigate and deal with illegal acts of adding thiazolidinones to hypoglycemic drugs and health care products without authorization. It involves a wide range and requires a large number of inspections. It is more difficult to carr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/33
Inventor 卞禹卜陶艳伟卫喜燕杨晓慧李林
Owner WUXI ZODOLABS BIOTECH