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Method for preparing soil remediation conditioner by using spore fermentative waste

A technology for fermentation waste liquid and soil remediation, applied in soil conditioning materials, chemical instruments and methods, applications, etc., can solve problems such as undeveloped, and achieve the effects of inhibiting breeding, promoting absorption and transformation, and easy to operate.

Inactive Publication Date: 2013-09-18
SHANGHAI CHUANGBO SOIL REMEDIATION ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it can be considered to use the spore fermentation waste liquid to improve the soil, but an effective method has not yet been developed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In the first step, the Pseudomonas species are inoculated on a special medium, and cultivated at 37° C. for 48 hours to obtain a Pseudomonas mother liquor.

[0034] Wherein, the special medium includes peptone 2wt%, beef extract 2wt%, yeast extract 1wt%, diammonium bisulfate 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, Tween 800.2wt%, dipotassium hydrogen phosphate 0.2wt%, magnesium sulfate 0.05wt%, manganese sulfate tetrahydrate 0.025wt%, iron sulfate heptahydrate 0.025wt%, calcium carbonate 0.1wt% and the rest water, and sterilized at 120°C for 20min.

[0035] In the second step, the prepared Pseudomonas mother solution is inoculated in the culture medium, and anaerobically fermented at 37° C. for 48 hours to obtain the Pseudomonas spore solution. Wherein, Pseudomonas accounted for 2.5wt% of the total mass of Pseudomonas and the medium; the medium included 5wt% molasses, 3wt% agar powder and the remaining spore fermentation waste liquid, and was sterilized at 120°C fo...

Embodiment 2

[0039] In the first step, Pseudomonas strains are inoculated on a special medium, and cultivated at 37° C. for 36 hours to obtain a Pseudomonas mother liquor.

[0040] Wherein, the special medium includes peptone 1wt%, beef extract 1wt%, yeast extract 0.5wt%, diammonium bisulfate 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, Tween 800.2wt%, dihydrogen phosphate Potassium 0.2wt%, magnesium sulfate 0.06wt%, manganese sulfate tetrahydrate 0.03wt%, iron sulfate heptahydrate 0.025wt%, calcium carbonate 0.1wt% and the rest of water, and sterilized at 120°C for 20min.

[0041] In the second step, the prepared Pseudomonas mother solution is inoculated in the culture medium, and anaerobically fermented at 37° C. for 36 hours to obtain the Pseudomonas spore solution. Wherein, Pseudomonas accounted for 0.5wt% of the total mass of Pseudomonas and the medium; the medium included 2wt% molasses, 1wt% agar powder and the remaining spore fermentation waste liquid, and was sterilized at 120°C fo...

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Abstract

The invention provides a method for preparing soil remediation conditioner by using spore fermentative waste. The method comprises the following steps: inoculating pseudomonas pseudoacaligenes into a culture medium, anaerobically culturing to obtain the pseudomonas pseudoacaligenes liquor, inoculating the pseudomonas pseudoacaligenes liquor into a fermentation culture medium, anaerobically culturing to obtain the soil remediation conditioner. The obtained remediation conditioner comprises a plurality of active microorganisms, enzymes, organic acids and antibiotics, and is capable of promoting the good micro-ecological environment in the crop soil, solubilizing the organic and inorganic nutrients, promoting the nutrient adsorption and conversion of the crop, improving the soil granular structure as well as inhibiting the breeding of the harmful pathogenic bacteria, and the pesticide residue harm in the soil is dissolved to a certain degree. The method provided by the invention is capable of reducing emission and protecting environment, and the nutrient component in the waste liquid can be sufficiently used for producing the soil remediation conditioner, the cost and water resource are saved, the operation is convenient, simple and easy, the operation cost is low, and the industrial application can be easily realized.

Description

technical field [0001] The invention relates to a method for preparing a soil remediation improver, in particular to a method for preparing a soil remediation improver by using spore fermentation waste liquid. Background technique [0002] Bacillus (Bacillaceae), a family of bacteria, bacilli or cocci that can form spores (endospores). Including Bacillus, Lactobacillus, Clostridium, Desulfurized Enterobacter and Bacillus sarcina, etc. They are highly resistant to external harmful factors, widely distributed, and exist in soil, water, air, and animal intestines. Bacillus A genus of bacteria in the Bacillus family. [0003] Studies have shown that Bacillus can produce a variety of digestive enzymes to help animals digest and absorb nutrients. Bacillus has strong protease, amylase and lipase activities, and also has enzymes that degrade complex carbohydrates in feed, such as pectinase, glucanase, cellulase, etc. These enzymes can destroy the cell wall of plant feed cells , ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/00C09K101/00
Inventor 江瀚陈仁爱
Owner SHANGHAI CHUANGBO SOIL REMEDIATION ENG
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