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Method for preparing hydrogel by enzymatic free radical polymerization

A free radical and hydrogel technology, which is applied in the field of hydrogel preparation by enzymatic free radical polymerization, can solve the problems of complex product purification in the reaction process and poor mechanical strength of hydrogel, and achieve simple operation, mild reaction conditions, and environmental protection. friendly effect

Inactive Publication Date: 2013-10-09
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of such hydrogels requires chemical modification of biological macromolecules (such as hyaluronic acid, alginate, gelatin, etc.) with specific chemical reagents (such as tyramine, p-hydroxyphenylpropionic acid, etc.) The reaction process and product purification are relatively complicated; and the prepared hydrogel generally has poor mechanical strength, and is usually used as an injectable medical material

Method used

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  • Method for preparing hydrogel by enzymatic free radical polymerization
  • Method for preparing hydrogel by enzymatic free radical polymerization
  • Method for preparing hydrogel by enzymatic free radical polymerization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Preparation of vinyl-modified bovine serum albumin: Take 600 mg of bovine serum albumin (BSA) and 54 mg of N-acryloyloxysuccinimide, dissolve them in 30 mL of deionized water, and react with magnetic stirring at room temperature for 2.5 hours. The reaction mixture was transferred to a dialysis bag, dialyzed in deionized water for 3 days, and freeze-dried to obtain a white solid product which was vinyl-modified bovine serum albumin. According to the method reported in the literature (J.Biosci.Bioeng., 2005, 100, 551-555), it is measured that on average, about 4 double bonds are modified on each BSA molecule.

[0028] 2) Preparation of precursor solution: Take 0.1021g of N,N-dimethylacrylamide, 0.0608g of vinyl-modified bovine serum albumin, and 1.7162g of deionized water into the sample bottle, mix well with a vortex mixer, and measure the pH value is 6.0.

[0029] 3) Preparation of hydrogel: 7.784 mg of acetylacetone, 100 μL of concentrated horseradish peroxidase so...

Embodiment 2

[0031] 1) Referring to Example 1, vinyl-modified bovine serum albumin was prepared and a precursor solution was prepared.

[0032]2) Preparation of hydrogel: Add 14.60 mg of acetylacetone, 100 μL of horseradish peroxidase concentrated solution, and 6 μL of aqueous hydrogen peroxide solution to the above precursor solution in sequence (the molar concentration ratio of acetylacetone and hydrogen peroxide in the reaction system is 5. The molar concentration ratio of hydrogen peroxide and horseradish peroxidase was 280), mixed quickly, and left in a closed place to obtain a light yellow transparent hydrogel with a gelation time of 58 s.

Embodiment 3

[0034] 1) Referring to Example 1, vinyl-modified bovine serum albumin was prepared and a precursor solution was prepared.

[0035] 2) Preparation of hydrogel: 36.01 mg of acetylacetone, 100 μL of concentrated horseradish peroxidase solution, and 6 μL of aqueous hydrogen peroxide solution were sequentially added to the above precursor solution (the molar concentration of acetylacetone and hydrogen peroxide in the reaction system was 12 , the molar concentration ratio of hydrogen peroxide and horseradish peroxidase is 280), mixed quickly, and left in airtight to obtain light yellow transparent hydrogel, gelation time 20s.

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Abstract

The invention relates to a method for preparing hydrogel by enzymatic free radical polymerization. According to the method, peroxidase, a beta-dicarbonyl compound and hydrogen peroxide are combined as an enzymatic free radical initiating system for providing free radicals for polymerization. The method comprises the following steps of: uniformly mixing an aqueous solution containing the peroxidase, the beta-dicarbonyl compound and alkene monomers in a using process; and then, adding hydrogen peroxide in the aqueous solution, adjusting the component concentration of a peroxidase / beta-dicarbonyl compound / hydrogen peroxide ternary initiating system, and controlling to obtain the hydrogel within 20 seconds to 30 minutes under the room temperature. The method for preparing the hydrogel by enzymatic free radical polymerization is realized in a water phase, gentle in reaction condition, simple and convenient to operate, quick and controllable in hydrogel forming time and capable of preparing the high-strength nanometer composite hydrogel material, and therefore, the method has an obvious application prospect in the fields including immobilized enzyme carriers, medicament controlled release, artificial cartilages, tissue engineering scaffold materials and the like.

Description

technical field [0001] The invention relates to the field of polymer hydrogels, in particular to a method for preparing hydrogels by enzymatic radical polymerization. Background technique [0002] Hydrogel is a three-dimensional network material capable of absorbing and retaining a large amount of water (usually with a water content greater than 70%), and its network skeleton is usually a natural or synthetic polymer molecular chain. Because it is very similar to the body tissue filled with a large amount of aqueous solution, hydrogels are widely used in the fields of biomedicine, biotechnology and biochemistry, including enzyme immobilization carriers, biosensing, wound dressings, drug release, tissue engineering (tissue filling) materials, articular cartilage, cell culture), etc. [0003] The preparation of polymer hydrogels is generally divided into two types: physical crosslinking and chemical crosslinking. Physical crosslinks are formed by non-covalent bonding forces ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L33/24C08L33/26C08L33/10C08L33/08C08L39/06C08F20/54C08F20/56C08F20/28C08F26/10C08K3/34C08K3/36C08K3/32
Inventor 苏腾王启刚
Owner TONGJI UNIV
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