Preparation technology and medical application of compound specific transfer factor
A transfer factor and preparation process technology, applied in the field of biomedicine, can solve the problems of narrow anti-infective disease spectrum, unstable activity and small scale, and achieve the effect of wide anti-infectious disease spectrum, stable product quality and large scale.
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Embodiment 1
[0025] Preparation of compound AIDS-specific transfer factor:
[0026] 1) After absorbing the nutrient solution, replace the donkey leukocyte strain with 1-2% seed solution with fresh culture solution, continue to cultivate at 37 degrees, and observe 5-7 days after inoculation, and about 80% of the cells have lesions , when there is a significant difference from the control cells, the virus content per milliliter is ≥105TCD50, and the same amount is respectively introduced into AIDS virus culture stock solution and BCG, and inactivated by hydrostatic pressure (300Mpa, 30min);
[0027] 2) Add 0.6% deketone nitrogen 101 to the total amount of virion stock solution, mix on a shaker for 1.5 hours, use a nano-collider to process under the conditions of 200-300 pressure and 20 nanometers, use gel filtration to remove ketone nitrogen 101, add 30 % fat emulsion is used as an immune adjuvant to obtain the lysed vaccine antigen;
[0028] 3) immunize 3 healthy horses and donkeys each,...
Embodiment 2
[0033] Preparation of compound specific transfer factor for dogs with pentavalent vaccine
[0034] The pentavalent vaccine for dogs includes five attenuated viruses including rabies, canine febrile fever, canine parainfluenza, canine adenovirus and canine parvovirus, which are kept, identified and supplied by the China Veterinary Drug Control Institute. The five kinds of viruses used include three grades of original virus seeds, basic virus seeds and production virus seeds, and the third-level virus seeds are all cell cultures adapted to the virus seeds, of which the 1-5 generation is the original virus seed; the 6-10 generation is the Basic poisonous species; 11-15 generation poisonous species for production. All three types of viruses are acceptable, and the original virus seed cell culture stock solution is the best.
[0035] Vaccines are mixed in batches of 10,000 doses, that is, each dose contains 15 minimum immune doses of rabies, canine distemper and canine parainflu...
Embodiment 3
[0042] Preparation of Specific Transfer Factor of Combined Bacteria
[0043] 1) Mix blue bacteria net and type b influenza "Haemophilus conjugate vaccine" in equal proportions, then add 30% fat emulsion to mix according to the ratio of the above total amount of 1:1, and sterilize with hydrostatic pressure 200Mpa for 20 minutes live;
[0044] 2) Add 0.5% deketone nitrogen 101 to the total amount of virion stock solution, mix on a shaker for 1 hour, use a nano-collider to process under the conditions of 250 pressure and 30 nanometers, remove ketone nitrogen 101 by gel filtration, and add 20% fat Milk is used as an immune adjuvant to obtain the specific transfer factor antigen of the lysed compound combined with bacteria;
[0045] 3) Select 4 horses and 10 pigs for the above-mentioned antigen immunization injection, collect the animal immune system organ tissue, crush the tissue through a meat grinder, add 2 times normal saline, make a homogenate with a homogenizer, filter wit...
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