Method for determination of doxorubicin hydrochloride in body fluid based on magnetic nano-adsorbent

A doxorubicin hydrochloride and magnetic nanotechnology, applied in chemical instruments and methods, measuring devices, alkali metal oxides/hydroxides, etc., can solve complex and cumbersome pretreatment problems, achieve high yield, mild conditions, Simple operation effect

Inactive Publication Date: 2013-12-25
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pretreatment for the detection of doxorubicin hydrochloride in body fluid samples is complex and cumbersome, therefore, it is particularly important to establish a simple, fast and effective method for the detection of doxorubicin hydrochloride in body fluid samples

Method used

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  • Method for determination of doxorubicin hydrochloride in body fluid based on magnetic nano-adsorbent
  • Method for determination of doxorubicin hydrochloride in body fluid based on magnetic nano-adsorbent
  • Method for determination of doxorubicin hydrochloride in body fluid based on magnetic nano-adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: TSAB-coated Fe 3 o 4 / SiO 2 Preparation of Magnetic Nanosorbents

[0022] (1) Weigh 0.994g FeCl 2 4H 2 O and 2.365 g FeCl 3 ·6H 2 O is placed in a 250mL three-necked bottle, add 0.4mL concentrated hydrochloric acid and 120mL secondary water to form a mixed solution, heat to 70°C, and under nitrogen protection, mechanically stir at 1000-1500rpm and slowly add 25% ammonia water dropwise to adjust the pH to 9 -10, continue to react for 2 hours to obtain a black suspension;

[0023] (2) Add 2.0 mL tetraethyl orthosilicate (TEOS) to the suspension obtained in step (1), and continue to react for 1.0 hour to obtain a brown-black suspension;

[0024] (3) Add 0.697g of trimethyloctadecylammonium bromide (TSAB) to the suspension obtained in step (2), and continue the reaction for 1.0 hour to obtain a brown suspension;

[0025] (4) Place the suspension obtained in step (3) to room temperature, then magnetically separate, wash the obtained solid with secondary wa...

Embodiment 2

[0027] Example 2: TSAB-coated Fe 3 o 4 / SiO 2 Preparation of Magnetic Nanosorbents

[0028] (1) Weigh 0.994g FeCl 2 4H 2 O and 2.365 g FeCl 3 ·6H 2 O was placed in a 250mL three-necked bottle, and 0.4mL of concentrated hydrochloric acid and 140mL of secondary water were added to form a mixed solution, heated to 70°C, mechanically stirred at 1000-1500rpm under nitrogen protection, and 25% ammonia water was slowly added dropwise to adjust the pH to 9 -10, continue to react for 2 hours to obtain a black suspension;

[0029] (2) Add 2.0 mL tetraethyl orthosilicate (TEOS) to the suspension obtained in step (1), and continue to react for 1.0 hour to obtain a brown-black suspension;

[0030] (3) Add 0.876g of trimethyloctadecylammonium bromide (TSAB) to the suspension obtained in step (2), and continue the reaction for 1.0 hour to obtain a brown suspension;

[0031](4) Place the suspension obtained in step (3) to room temperature, then magnetically separate, wash the obtained...

Embodiment 3

[0033] Example 3: TSAB-coated Fe 3 o 4 / SiO 2 Preparation of Magnetic Nanosorbents

[0034] (1) Weigh 0.994g FeCl 2 4H 2 O and 2.365 g FeCl 3 ·6H 2 O is placed in a 250mL three-necked bottle, add 0.4mL concentrated hydrochloric acid and 160mL secondary water to form a mixed solution, heat to 70°C, and under nitrogen protection, mechanically stir at 1000-1500rpm and slowly add 25% ammonia water dropwise to adjust the pH to 9 -10, continue to react for 2 hours to obtain a black suspension;

[0035] (2) Add 2.0 mL tetraethyl orthosilicate (TEOS) to the suspension obtained in step (1), and continue to react for 1.0 hour to obtain a brown-black suspension;

[0036] (3) Add 1.044g of trimethyloctadecylammonium bromide (TSAB) to the suspension obtained in step (2), and continue the reaction for 1.0 hour to obtain a brown suspension;

[0037] (4) Place the suspension obtained in step (3) to room temperature, then magnetically separate, wash the obtained solid with secondary wa...

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Abstract

The invention provides a preparation method of a trimethylstearylammonium bromide (TSAB)-coated Fe3O4 magnetic nano-adsorbent, and a method for determination of doxorubicin hydrochloride in blood plasma and urine by the TSAB-coated Fe3O4 magnetic nano-adsorbent. The method for determination of doxorubicin hydrochloride in blood plasma and urine comprises the following steps of preparing the TSAB-coated Fe3O4 / SiO2 in a layer-by-layer assembly way by a chemical coprecipitation method, adding the TSAB-coated Fe3O4 / SiO2 into doxorubicin hydrochloride-containing blood plasma and urine samples, adjusting a pH value to 4-5 by a phosphate buffer, carrying out vortex oscillation for 3min, carrying out magnetic separation, removing a supernatant, adding an eluent into the magnetic separation product, carrying out elution, carrying out magnetic separation, collecting a supernatant and carrying out analysis by a high performance liquid chromatography-fluorescence detection method. The magnetic nano-adsorbent obtained by the preparation method has good stability and large adsorption quantity, can be recycled, has a strong capability of extracting anthracene ring antitumor antibiotics such as adriamycin from blood plasma and urine samples, and can realize fast and effective separation and determination of doxorubicin hydrochloride in the samples.

Description

technical field [0001] The invention relates to a preparation method of a magnetic nano-adsorbent and a detection method of doxorubicin hydrochloride, specifically a trimethyloctadecylammonium bromide (TSAB) modified Fe 3 o 4 A preparation method of a magnetic nanometer adsorbent, and a method for determining doxorubicin hydrochloride in blood plasma and urine by using the adsorbent prepared by the method. Background technique [0002] In recent years, Fe 3 o 4 The synthesis and application of magnetic nanoparticles and their complexes are research hotspots. It has the characteristics of small particle size, large specific surface area, high coupling capacity and superparamagnetism, and can be oriented to aggregate under the action of an external magnetic field, so it shows broad application prospects in the fields of medicine, chemistry, environment and biology. In addition, Fe 3 o 4 Magnetic nanoparticles can be easily modified on the surface to have special properti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30G01N30/02
Inventor 李瑞金麻能轩张林孙琳琳牛卫芬周叶红双少敏
Owner SHANXI UNIV
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