Preparation method and application of onionskin flavones
A technology of flavonoids and onion skins, which is applied in the field of preparation of flavonoids from onion skins, to achieve the effects of promoting rapid development, meeting market demand, and improving overall benefits
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[0015] A kind of preparation method of onion flavonoids of the present invention comprises the following steps:
[0016] 1) Take 3kg of onion skin, add 40-60L of distilled water, heat to 67-70°C, keep warm for 2-8h, filter, pour out the supernatant to obtain filter residue;
[0017] 2) Add 40-60L of 70% ethanol to the filter residue obtained in step 1), heat to 67-70°C, keep warm for 2-8h, and filter to obtain supernatant and residue;
[0018] 3) concentrating the supernatant obtained in step 2) and drying to obtain onion flavonoids;
[0019] 4) drying the residue obtained in step 2) to obtain onion dietary fiber.
[0020] The step can also be: take the onion skin and add 80-100 U / g of cellulase and 80-100 U / g of pectinase, and perform compound enzymatic hydrolysis for more than 1 hour under the conditions of pH 4-5 and temperature 40-50° C. to obtain onion skin flavonoids.
Embodiment 1
[0022] 1) Take 3kg of onion skin, add 50L of distilled water, heat to 70°C, keep warm for 6h, filter, pour out the supernatant, and obtain the filter residue;
[0023] 2) Add 50 L of 70% ethanol to the filter residue obtained in step 1), heat to 70° C., keep it warm for 4 hours, and filter to obtain supernatant and residue;
[0024] 3) concentrating the supernatant obtained in step 2) and drying to obtain onion flavonoids;
[0025] 4) drying the residue obtained in step 2) to obtain onion dietary fiber.
Embodiment 2
[0027] Take 3 kg of onion skin and add 90 U / g of cellulase and 90 U / g of pectinase to carry out compound enzymatic hydrolysis for 1.5 h under the conditions of pH 4.5 and temperature 45° C. to obtain flavonoids from onion skin.
[0028] Wherein, the effect of gained onion brass is verified by the following experiments:
[0029] Step 1: Prepare materials
[0030] 40 SPF grade male SD rats (body weight 100±10g) were purchased from the Experimental Animal Center of Yanbian University; onion powder (moisture content below 5%) was made by our laboratory; tea polyphenols with a purity of 95% were purchased from Hangzhou Premier Biotechnology Co., Ltd.; basic feed purchase and Yanbian University Experimental Animal Center; nutritional feed self-made in this laboratory (80% basic feed, 10% lard, 10% egg yolk powder), FA1104 analytical balance; TDL-40B low-speed desktop large capacity Centrifuge; HH-6 digital display constant temperature water bath; Roche excellent blood glucose meter...
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