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Application of reverse micelle system in catalysis of lignin peroxidase for esterification reaction of oleic acid

A technology of peroxidase and reverse micelles, applied in the field of treatment of oleic acid pollution, to achieve the effect of mild production conditions, mild reaction conditions, and simple production equipment

Inactive Publication Date: 2014-02-12
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reverse micellar solution is disclosed in the above patent, but it is used to extract lignin peroxidase

Method used

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  • Application of reverse micelle system in catalysis of lignin peroxidase for esterification reaction of oleic acid
  • Application of reverse micelle system in catalysis of lignin peroxidase for esterification reaction of oleic acid
  • Application of reverse micelle system in catalysis of lignin peroxidase for esterification reaction of oleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use a pipette to take 500ml each of isooctane and n-hexanol into a beaker, take 4.1448g of rhamnolipid in a 1L volumetric flask to volume, and ultrasonically dissolve until the solution is clear to obtain a 100CMC RL-isooctane-n-hexanol solution.

[0020] 1.5 mg of lignin peroxidase was dissolved in 15 ml of disodium hydrogen phosphate-citric acid buffer solution with pH=3 to prepare a crude enzyme solution. Dissolve 1.782ml of crude enzyme solution in 10ml of the above RL-isooctane-n-hexanol solution to form a reverse micellar enzyme system, wherein the molar ratio (Wo) of water / rhamnolipid is 30. Add 40mmol oleic acid and 10mmol ethanol to the reverse micelles enzyme system, put it into a water bath shaking box, the temperature is 25°C, the rotation speed is 150rpm, and the reaction time is one hour.

[0021] Every 10 minutes, take 1ml of the reaction product, add 1ml of copper acetate (containing pyridine with a pH of 6.1) and 5ml of benzene, and centrifuge in a cent...

Embodiment 2

[0023] Use a pipette to take 500ml each of isooctane and n-hexanol into a beaker, take 2.1448g of rhamnolipid in a 1L volumetric flask to volume, ultrasonically dissolve until the solution is clear, and obtain a 20CMC RL-isooctane-n-hexanol solution.

[0024] Dissolve 5.4 mg of lignin peroxidase enzyme in 50 ml of disodium hydrogen phosphate-citric acid buffer solution with pH=2 to prepare a crude enzyme solution. Dissolve 2.376ml of crude enzyme solution in 10ml of the above-mentioned RL-isoctane-n-hexanol solution to form a reverse micellar enzyme system, wherein the molar ratio (Wo) of water / rhamnolipid is 40, and the Add 60mmol oleic acid and 10mmol ethanol to the mixture, put it into a water bath shaking box, the temperature is 40°C, the rotation speed is 150rpm, and the reaction time is one hour.

[0025] Every 10 minutes, take 1ml of the reaction product, add 1ml of copper acetate (containing pyridine with a pH of 6.1) and 5ml of benzene, and centrifuge in a centrifuge ...

Embodiment 3

[0027] Use a pipette to take 500ml each of isooctane and n-hexanol into a beaker, take 3.2418g of rhamnolipid in a 1L volumetric flask to volume, ultrasonically dissolve until the solution is clear, and obtain a 40CMC RL-isooctane-n-hexanol solution.

[0028] Take 4 mg of lignin peroxidase and dissolve it in 10 ml of disodium hydrogen phosphate-citric acid buffer solution with pH=5 to prepare a crude enzyme solution. Dissolve 1.934ml of crude enzyme solution in 10ml of the above RL-isooctane-n-hexanol solution to form a reverse micellar enzyme system, wherein the molar ratio (Wo) of water / rhamnolipid is 34. Add 57mmol oleic acid and 14mmol n-propanol to the reverse micellar enzyme system, put it into a water bath shaking box, the temperature is 32°C, the rotation speed is 150rpm, and the reaction time is 2 hours.

[0029] Every 10 minutes, take 1ml of the reaction product, add 1ml of copper acetate (containing pyridine with a pH of 6.1) and 5ml of benzene, and centrifuge in a ...

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Abstract

The invention discloses an application of a reverse micelle system in the catalysis of lignin peroxidase for an esterification reaction of an oleic acid. The reverse micelle system consists of rhamnoilpid, iso-octane and n-hexanol, wherein the ratio of the iso-octane to the n-hexanol is 1:(1-3); the clinical micelle of the reverse micelle system is 20 to 100 CMC. The application comprises the following steps of mixing crude enzyme of the lignin peroxidase and the reverse micelle system to form a reverse micelle enzyme system, adding the oleic acid and C1-C10 organic alcohol, and performing water bath shaking to obtain an esterification product, wherein the crude enzyme of the lignin peroxidase consists of lignin peroxidase and a buffer solution. The application of the reverse micelle system in the catalysis of the lignin peroxidase for the esterification reaction of the oleic acid is environment-friendly, safe, convenient to operate, low in cost and high in efficiency and yield.

Description

technical field [0001] The invention relates to a method for treating oleic acid pollution, in particular to the application of a reverse micellar system in the oleic acid reaction catalyzed by lignin peroxidase. Background technique [0002] Oleic acid has been widely debated due to its serious environmental pollution and ecological hazards. Therefore, the effective esterification of oleic acid is an important prerequisite for the resource utilization of a large amount of waste oleic acid biomass in the environment. And ethyl oleate is widely used as the subject fragrance ingredient of cosmetics and perfumes. Lignin peroxidase (Lip) has a wide range of substrates and strong oxidizing ability. Using this enzyme to degrade various pollutants has become a research hotspot in environmental pollution control in the world today. Under normal water system conditions, the solubility of long-chain acids is relatively poor, which is not conducive to the contact of enzymes with subst...

Claims

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Application Information

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IPC IPC(8): C12P7/62
Inventor 袁兴中包姗彭馨刘欢马玉洁吴秀莲吴志斌曾光明
Owner HUNAN UNIV
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