Asymmetry oxamide fiber-bridged three-kernel copper complex as well as preparation method and application thereof
A technology of oxamide bridge and copper complex is applied in copper organic compounds, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effects of high yield, wide application range and high antitumor activity
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Embodiment 1
[0026] The preparation method of the asymmetric oxamide heterometallic one-dimensional coordination polymer of the present invention comprises the following steps:
[0027] 1. Preparation of unsymmetrical oxamide ligand N-(2-carboxyphenyl)-N'-[(2-aminoethyl)ethyl]oxamide
[0028] Oxamide ligand N-(2-carboxyphenyl)-N'-[(2-aminoethyl)ethyl]oxamide (H 3 The preparation method of bdeox) is as follows: Weigh 10 mmol of N-(2-carboxyanilino) ethyl oxalyl ester, dissolve it in 10 mL of tetrahydrofuran / ethanol (v:v=1:1) mixed solution, stir at 0°C, and It was added dropwise to 10 mL of a tetrahydrofuran / ethanol (v:v=1:1) mixed solution containing 14 mmol of diethylenetriamine. After the addition was complete, the reaction was continued to stir in an ice bath for 6 hours, and a large amount of white solid was formed. Suction filtration, washing the filter cake with absolute ethanol and anhydrous ether several times successively, and vacuum drying at room temperature to obtain a white ...
Embodiment 2
[0047] Example 2: Cell experiment
[0048] (1) Experimental group:
[0049] Adherent tumor cell lymphoid leukemia cells P388 and human liver cancer cells BEL7404 in the logarithmic growth phase were selected, digested with trypsin, and prepared a cell suspension of 50,000 cells / mL with RPM11640 culture medium with 10% calf serum, and inoculated in Inoculate 100 μL per well in 96 empty culture plates, at 37°C, 5% CO 2 , and cultured for 24 hours.
[0050] Add 10 μL of the obtained coordination polymer solution (dissolve the coordination polymer crystals with DMSO, dilute with physiological saline to a final concentration of 5 μg / mL), make the final volume of each well 200 μL, and make up with 1640 culture medium. 37°C, 5% CO 2 , cultured for 3 days. After culturing for 3 days, discard the supernatant, add 100 μL of freshly prepared 0.5 mg / mL MTT (thiazolium blue) serum-free culture solution to each well, continue culturing at 37°C for 4 hours, carefully discard the supernat...
Embodiment 3
[0058] Example 3: Research on the interaction with HS-DNA
[0059] UV-Vis Absorption Spectroscopy Titration
[0060] In the UV-visible spectrum test, add the same volume of Tris-HCl buffer solution and ligand solution to the reference cell and the sample cell, fix the ligand concentration, and scan the UV-visible spectrum in the range of 200-600nm. Add 12 μL of HS-DNA (5.16×10 -3 mol L -1 ) solution, so that the concentration ratio of DNA and ligand increases continuously until it is saturated, and the absorption value of the ligand does not change. Combining constant calculations:
[0061] [DNA] / (εa-εf)=[DNA] / (εb-εf)+1 / Kb(εb-εf)
[0062] Where εa is the molar extinction coefficient of the solution at any DNA concentration; εf is the molar extinction coefficient of the free complex; εb is the molar extinction coefficient when the complex is fully bonded to DNA; [DNA] is plotted to give a slope and intercept, the ratio of which is the binding constant Kb.
[0063] Experim...
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