A preparation method of photoelectric aptasensor for detecting 17β-estradiol

An aptamer sensor, estradiol technology, applied in the direction of material electrochemical variables, etc., can solve the problems of limiting direct application, inactivation of biomolecules, etc., and achieve good biocompatibility, high sensitivity, and improved selectivity

Inactive Publication Date: 2016-02-17
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to TiO 2 NTs have a wide band gap and can only absorb visible light below 400 nm, which will make the modification on TiO 2 The inactivation of biomolecules on the surface of NTs limits its direct application in the design of biophotonic sensors to some extent, and requires the use of narrow-band optoelectronic materials and TiO 2 NTs recombination for biosensing under visible light

Method used

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  • A preparation method of photoelectric aptasensor for detecting 17β-estradiol
  • A preparation method of photoelectric aptasensor for detecting 17β-estradiol
  • A preparation method of photoelectric aptasensor for detecting 17β-estradiol

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Embodiment 1

[0026] A highly sensitive and selective photoaptamer sensor for 17β-estradiol constructed on CdSeNPs-TiO 2 NTs as the substrate, the aptamer of 17β-estradiol was modified on CdSeNPs-TiO 2 Inside and outside of the tube of NTs. The specific production process is as follows:

[0027] (1) The titanium plate is mechanically polished with sandpaper of different fineness in turn, and then placed in double distilled water and ethanol solution for ultrasonic cleaning for 5-10 minutes respectively. Before anodization, the titanium plate was etched with 18% HCl for 10 minutes, and then placed in a two-electrode system for anodization. The platinum plate was used as the cathode, and the titanium plate was used as the anode. The distance between the two electrodes was 1 cm, and the applied cell voltage was 40V. When the temperature is 25°C, anodize for 2 to 3 hours. The electrolyte contains 0.3wt%NH 4 F and 3.0Vol%H 2 O solution in ethylene glycol. Anodized TiO 2 NTs were rinsed wi...

Embodiment 2

[0031] After preparing 1mmol / L 17β-estradiol stock solution with absolute ethanol as solvent, it was diluted with 0.1mol / LPBS buffer solution (pH7.41) to prepare a series of 17β-estradiol standard solutions with different concentrations. A buffer solution of 0.1mol / LPBS (pH7.41) and 0.1mol / L ascorbic acid (AA) is used as the electrolyte, where AA is used as an electron sacrificial agent. At a voltage of 0.0 V, the electrodes were kept 5 cm from the visible light source. CdSeNPs-TiO modified with nucleic acid aptamers 2 NTs is the working electrode, the saturated calomel electrode is the reference electrode, and the platinum wire is the counter electrode. In the three-electrode system, when different concentrations of 17β-estradiol are added to the electrochemical cell, each sample needs to be reacted for 30 minutes and then amperometric i-t curve determination. Due to the specific recognition of the aptamer and its target substance, 17β-estradiol is captured on the electrode...

Embodiment 3

[0033] CdSeNPs-TiO modified with 17β-estradiol aptamer 2 NTs were placed in 0.1mol / LPBS+0.1mol / LAA (pH7.41) as electrolyte, and 1.0×10 -12 mol / L17β-estradiol was mixed with different concentrations of interfering substances in pairs for determination. The interference of six environmental endocrine disruptors, ethinyl estradiol, estriol, nonylphenol, resorcinol, bisphenol A, atrazine and diethyl phthalate, on the determination of 17β-estradiol was investigated experiment. Using the test conditions in Example 2, measure the photocurrent response, the research results show that the influence caused by the photocurrent of other interfering substance 17β-estradiol which is 100 times the concentration of 7β-estradiol is less than 10.0%, such as image 3 shown. It can be seen that the prepared aptasensor has high selectivity to 17β-estradiol, and other six environmental endocrines with similar structures or coexisting with 17β-estradiol do not interfere with its determination.

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Abstract

The invention relates to a method for preparing a photoelectric adapter sensor for detecting 17beta-estradiol. The photoelectric adapter sensor takes a prepared cadmium selenide nano particle modified titanium dioxide nano tube as a matrix electrode; after the surface of the electrode is functionalized by chitosan and glutaraldehyde, and a 17beta-estradiol adapter is dropwise added onto the surface of the electrode so as to prepare the 17beta-estradiol photoelectric adapter sensor. Compared with the prior art, the method combines the 17beta-estradiol photoelectric adapter sensor with specific recognition capability with a supersensitive photoelectric analysis technique for detecting an environmental internal-secretion interfering-substance 17beta-estradiol, so that not only is high sensitivity acquired, but also interference of a complex matrix environment is prevented, and high selectivity is achieved; when the method is used in low-concentration 17beta-estradiol detection, a wide working curve range of 5*10<-13> mol / L to 15*10<-12> mol / L is obtained, the minimum detection limit can be 3.3*10<-14> mol / L, and moreover, the 17beta-estradiol photoelectric adapter sensor is simple in preparation method, low in cost and good in stability and repeatability.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring and nano-modified electrodes, in particular to a method for preparing a photoelectric aptasensor for detecting 17β-estradiol. Background technique [0002] Endocrine disruptors have a serious impact on the endocrine function of the human body and other organisms, and have attracted much attention in recent years. Among them, 17β-estradiol is considered to be the most harmful environmental endocrine to the endocrine system of humans and animals. It is a natural female hormone, mainly derived from the excrement of humans or livestock, as well as the use and discharge of drugs containing synthetic hormones. When 17β-estradiol enters the human body and other organisms from the external environment through the food chain, it will interfere with normal physiological processes, affect human growth and development, reduce the male birth rate, and increase the incidence of tumors. In recen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
Inventor 赵国华范丽芳史慧杰刘梅川
Owner TONGJI UNIV
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