Method for preparing trehalose and yeast extract by utilization of waste beer yeast

A technology for waste beer yeast and yeast paste, which is applied in the preparation of sugar derivatives, chemical instruments and methods, disaccharides, etc., can solve the problems of high price, microbial pollution, limitations, etc., and achieve the effect of high utilization value and rich nutrition

Active Publication Date: 2014-08-20
HEZE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yeast extract produced by the autolysis method has high protein decomposition rate, high content of free amino acids, good flavor, and low cost, but the content of flavor nucleotides is low, and its lysate is often difficult to separate from solid and liquid, and it is easy to Contamination by other microorganisms
In the traditional process, inorganic salts (such as sodium chloride or some non-polar organic solvents) are generally added to accelerate the cracking, but when the yeast powder is extracted from the autolyzed supernatant, desalination or detoxification will be introduced and other complex processes
In addition, other enzymatic hydrolysis methods are used in some processes to accelerate autolysis, but due to the high cost, they can only be limited to laboratory analysis

Method used

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  • Method for preparing trehalose and yeast extract by utilization of waste beer yeast
  • Method for preparing trehalose and yeast extract by utilization of waste beer yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as figure 1 Shown, a kind of method utilizing beer waste yeast to prepare trehalose and yeast extract comprises the following steps:

[0028] 1) Washing, debittering and purification

[0029] Take the waste beer yeast mud, add pure water to the waste beer yeast mud, filter twice through 80~100 mesh sieve to obtain yeast suspension; add tartaric acid to the yeast suspension so that the mass fraction of tartaric acid in the solution is 5%, stir After debittering for 60 min, centrifuge at 4500 rpm for 10 min, discard the supernatant to obtain purified yeast sludge.

[0030] 2) Autolysis

[0031] Add pure water, chitosan acetic acid solution (1.5g chitosan dissolved in 100mL 2% acetic acid solution) and 5'-phosphodiesterase enzyme solution to the purified yeast sludge obtained in step 1) to adjust the pH value of 6.5, stirred at 45 ° C for 15 h to autolyze; the amount of pure water added was 3 times the quality of the purified yeast slime, and the amount of chitosan...

Embodiment 2

[0037] Such as figure 1 Shown, a kind of method utilizing beer waste yeast to prepare trehalose and yeast extract comprises the following steps:

[0038] 1) Washing, debittering and purification

[0039] Take the waste beer yeast sludge, add pure water to the waste beer yeast sludge, filter through 80~100 mesh sieve three times to obtain yeast suspension; add tartaric acid to the yeast suspension so that the mass fraction of tartaric acid in the solution is 7%, stir for 50min After debittering, centrifuge at 4000 rpm for 15 min, discard the supernatant to obtain purified yeast sludge.

[0040] 2) Autolysis

[0041]Add pure water, chitosan acetic acid solution (1.5g chitosan dissolved in 100mL 2% acetic acid solution) and 5'-phosphodiesterase enzyme solution to the purified yeast sludge obtained in step 1) to adjust the pH value was 5.5, and stirred at 38°C for 15 h to autolyze; the amount of pure water added was 5 times the mass of the purified yeast slime, and the amount o...

Embodiment 3

[0047] Such as figure 1 Shown, a kind of method utilizing beer waste yeast to prepare trehalose and yeast extract comprises the following steps:

[0048] 1) Washing, debittering and purification

[0049] Take the waste beer yeast sludge, add pure water to the waste beer yeast sludge, and filter twice through a 80-100 mesh sieve to obtain a yeast suspension; add sodium bicarbonate to the yeast suspension, so that the mass fraction of sodium bicarbonate in the solution is 0.2%, after stirring for 60 minutes to remove bitterness, centrifuge at 4000 rpm for 15 minutes, discard the supernatant to obtain purified yeast sludge.

[0050] 2) Autolysis

[0051] Add pure water, chitosan acetic acid solution (1.5g chitosan dissolved in 100mL 2% acetic acid solution) and 5'-phosphodiesterase enzyme solution to the purified yeast sludge obtained in step 1) to adjust the pH value was 4.5, and stirred at 35°C for 20 h to autolyze; the amount of pure water added was 4 times the mass of the ...

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Abstract

The invention belongs to the technical field of biochemical engineering and discloses a method for preparing trehalose and yeast extract by the utilization of waste beer yeast. The method comprises the following steps: firstly washing, debitterizing and purifying waste beer yeast, adding an acetic acid solution of chitosan and 5'-phosphodiesterase liquid for autocytolysis of yeast cell after purification; centrifuging after autocytolysis of yeast cell; collecting a precipitate; carrying out ultrafiltration on a supernatant obtained by centrifugation, dialyzing a ultrafiltration permeate through a dialysis tube or deionizing and decolouring through exchange resin; extracting a dialysis concentrated solution or an eluant in ethyl alcohol; drying to obtain trehalose; and carrying out autocytolysis on a ultrafiltration concentrated solution, a dialysis eluate and yeast cell, centrifuging, mixing precipitates obtained by centrifugation, adding a proper amount of starch so as to prepare the yeast extract. By the method for preparing the yeast extract, only trehalose in waste beer yeast cell is removed, protein, nucleic acid and polysaccharides substances are not lost, and the prepared yeast extract has rich nutrients. The prepared yeast extract has high value in use and can be used for culturing a plurality of microbes.

Description

technical field [0001] The invention relates to the technical field of biochemical industry, in particular to a method for preparing trehalose and yeast extract by using waste beer yeast. Background technique [0002] Beer waste yeast is rich in polysaccharides such as protein, nucleic acid, vitamins, minerals, trehalose, mannan and dextran. In the traditional method, the waste yeast sludge is directly removed from impurities and bitterness to obtain clean yeast sludge, and dried yeast powder; or the clean yeast sludge is autolyzed with a solvent, and the precipitate is centrifuged to produce polysaccharides after autolysis, which is then centrifuged. The supernatant is usually let off directly, which not only wastes resources but also causes environmental pollution. [0003] The yeast extract produced by the waste beer yeast after enzymatic lysis or autolysis is an excellent natural seasoning, which can improve the flavor and quality of products when added to various foods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/04C07H1/06C12N1/06
Inventor 王宜磊王文爽杨宏宇张明
Owner HEZE UNIV
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