Sperm optimal selection device based on microtubule passages and optimal selection method

A sperm and channel technology, applied in the field of sperm selection, can solve the problems of sperm DNA peroxidation damage, sperm quality is difficult to control, and sperm quality decline, so as to avoid sperm quality decline, sperm quality is easy to control, and the screening effect is good Effect

Inactive Publication Date: 2014-10-15
ZHEJIANG CELLPRO BIOTECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The recovery rate of high-quality sperm is increased after density gradient centrifugation, but it is easy to cause peroxidative damage to sperm DNA and reduce the quality of sperm
The invention patent with the Chinese patent announcement number CN102352305 discloses the use of microfluidic core technology to detect and screen sperm. Buffer is added to the buffer sample pool, and the high-motility sperm in the sample pool is brought to the sample collection pool through the buffer flow to achieve the screening and collection of high-motility sperm. However, the patented technology of this invention is relatively complicated. Mucus is adsorbed, and the buffer is required to passively flush the sperm flow to the sample collection pool. The sperm is not actively flowing, and the quality of the sperm is not easy to control

Method used

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  • Sperm optimal selection device based on microtubule passages and optimal selection method
  • Sperm optimal selection device based on microtubule passages and optimal selection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Such as figure 1 A sperm optimization device based on a three-dimensional microtube channel is shown, including a sample pool 1 and a sperm collection pool 2, and a first-level barrier block 3 is arranged between the sample pool 1 and the sperm collection pool 2, and the barrier block 3 is covered with The microtube channel 4 from the sample pool 1 to the sperm collection pool 2 is filled, and the inner diameter of the microtube channel 4 is 10-200 microns (for example, human sperm can be set to about 50 microns).

Embodiment 2

[0017] It is basically the same as the embodiment 1, except that three levels of barrier blocks 3 are arranged between the sample pool 1 and the sperm collection pool 2, and an intermediate pool 5 is between two adjacent barrier blocks 3 . A three-stage barrier block 3 may also be provided, which will not be listed here.

Embodiment 3

[0019] A method for optimizing sperm by using the sperm optimization device of Example 1, first move the sperm culture fluid into the sperm collection pool, the sperm optimization device can be slightly inclined to the sample pool, so that the sperm culture fluid can pass through the microtube channel quickly, and then transfer the sperm Moved into the sample pool, the sperm culture solution contains sodium chloride with a concentration of 0.1mol / L, potassium dihydrogen phosphate with a concentration of 0.4mmol / L, glucose with a concentration of 2.7mmol / L, and chlorine with a concentration of 4.7mmol / L. Potassium chloride, magnesium sulfate heptahydrate with a concentration of 0.2mmol / L, calcium chloride dihydrate with a concentration of 2mmol / L, Hepes-Na with a concentration of 0.02mol / L, sodium bicarbonate with a concentration of 4mmol / L, and 0.3mmol / L sodium pyruvate, volume percent concentration of 0.34% C 3 h 5 o 3 Na, the Phenol Red that the mass percent concentration ...

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Abstract

The invention discloses a sperm optimal selection device based on microtubule passages. The sperm optimal selection device comprises a sample tank and a sperm collection tank, obstruction blocks in one to three stages are arranged between the sample tank and the sperm collection tank, microtubule passages are overspreaded on the obstruction blocks in a direction from the sample tank to the sperm collection tank, the inner diameters of the microtubule passages are 10-200 micrometer, high-quality and high-activity sperm preferentially reach the sperm collection tank by using the initiative attracting of a sperm nutrient solution to sperms, the blocking of the obstruction blocks, and microporous competitive passing, and under an effect of activity competition, the sperms in the sperm collection tank are highly energetic. According to the method provided by the invention, the selection is simple, the sperm recovering ratio is high, sperm quality reduction is avoided at the same time, and the selecting effect is good. Therefore, sperm optimal selection device based on microtubule passages has the advantages of simple selection, good selecting effect, high recovering rate, and easiness in sperm quality control.

Description

technical field [0001] The invention relates to sperm selection, in particular to a sperm selection device and method based on microtubules. Background technique [0002] Sperm motility is one of the key factors for conception. Semen with a high sperm motility rate has a relatively high number of motility sperm, so the probability of conception is also high. The currently widely used sperm selection technologies include upstream technology, density gradient centrifugation technology and other optimization technologies. The upstream technology is designed by using the principle of sperm’s own vitality to swim upwards. During the upstream process, sperm can break away from some endogenous antisperm substances in the seminal plasma, such as anti-sperm antibodies, various cells, etc., and make the sperm The time of in vitro activity can be extended, but the recovery rate of high-quality sperm is relatively low in the upstream technology. The recovery rate of high-quality sperm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/02
CPCC12M23/16C12M45/06C12N5/061C12N2509/00
Inventor 曾桥薛志刚
Owner ZHEJIANG CELLPRO BIOTECH
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