Carboxylesterase as well as coding gene and application thereof

A carboxylesterase and gene technology, applied in the field of genetic engineering, can solve problems such as difficult mass production, limited enzyme production capacity of wild strains, long fermentation cycle, etc., achieve good activity and stability, easy to obtain pure protein, and short cultivation cycle short effect
CN104109659AInactive Publication Date: 2014-10-22YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2014-10-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a carboxylesterase, a gene encoding the carboxylestearase, a recombination carrier comprising the gene, a recombination strain comprising the gene, and application of the carboxylesterase in degrading diisobutyl phthalate, wherein the amino acid sequence of the carboxylesterase is shown as SEQ ID NO.1. The carboxylesterase gene CarEW comes from a hot spring environment, is proven to have good activity and stability under the condition of 45DEG C, and can be used for degrading phthalate ester compounds (phthalate) such as diisobutyl phthalate in the environment, thus having important significance on the aspect of environment pollution treatment.
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Description

Technical field

[0001] The invention belongs to the technical field of genetic engineering, and particularly relates to a carboxylesterase, its coding gene and its application. Background technique

[0002] Diisobutyl phthalate (DIBP) is a phthalate compound and is a plasticizer and softener widely used in the plastics industry. Its role is to increase the plasticity and toughness of plastics and improve the strength of plastics (Zhao Zhenhua , Environmental Chemistry, 1991, 10(3): 64-68). In addition, DIBP is also widely used in the production process of coatings, printing and dyeing, cosmetics and fragrances. Recent studies have shown that DIBP only relies on intermolecular interactions to combine with polymer plastics. Over time, such substances can migrate directly into food through food packaging materials, or into the environment through plastic products containing the substance. This causes pollution to air, water, soil and food, and can enter the human body through brea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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