Method for detecting trace zinc ions by using cloud point extraction and ultraviolet-visible spectrophotometry

A spectrophotometry, zinc ion technology, applied in the measurement of color/spectral characteristics, preparation of test samples, etc., can solve the problems of time-consuming, labor-intensive, environmental pollution, etc. simple effect

Inactive Publication Date: 2014-12-10
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional extraction techniques and other traditional separation methods are time-consuming and labor-intensive. In addition, a large amount of high-purity organic solvents are required, causing serious pollution to the environment.

Method used

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  • Method for detecting trace zinc ions by using cloud point extraction and ultraviolet-visible spectrophotometry
  • Method for detecting trace zinc ions by using cloud point extraction and ultraviolet-visible spectrophotometry
  • Method for detecting trace zinc ions by using cloud point extraction and ultraviolet-visible spectrophotometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Establishment of cloud point extraction-ultraviolet-visible spectrophotometry method for determining trace zinc ions

[0030] 1. Instruments and reagents

[0031] (1) Instruments: pHS-3C digital acidity meter (Shanghai Ohaus Instrument Co., Ltd.), CAV214C electronic balance (Shanghai Ohaus Instrument Co., Ltd.), DL-360E intelligent ultrasonic cleaner (Shanghai Zhixin Instrument Co., Ltd. ), UV2550 ultraviolet-visible spectrophotometer (Shimadzu Corporation), TG6A centrifuge (Shanghai Lu Xiangyi Instrument Co., Ltd.), constant temperature blast drying oven (Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.), electric heating constant temperature water bath (Beijing Yong Guangming Medical Instrument Factory).

[0032] (2) Reagent: 1mg·mL -1 Zn 2+ Add a certain amount of 2% (v / v) dilute nitric acid solution to the standard stock solution, Zn 2+ Standard working solution (5 μg mL -1 ) is composed of Zn 2+ Standard stock solution diluted with water....

Embodiment 2

[0072] Example 2 The influence of interfering ions on the method of cloud point extraction-ultraviolet-visible spectrophotometry for the determination of trace zinc ions

[0073] According to the method of embodiment 1, the influence of several common metal ions on the extraction rate of zinc ions has been studied, and the results are shown in table 1. The metal ions in normal water samples, soil samples, and blood samples do not substantially affect the extraction rate of zinc ions. interference.

[0074] Table 1 Effect of interfering ions on cloud point extraction

[0075]

Embodiment 3

[0076] The mensuration of embodiment 3 samples

[0077] According to the method of Example 1, trace amounts of zinc ions in tap water, river water (Xihe), river water (Jialing River), mountain soil, garden soil, farmland soil and rabbit blood samples were determined. The results are shown in Table 2. The recoveries of the samples were in the range of 97.7-101.5%. Therefore, the combination of cloud point extraction and ultraviolet spectrophotometry can be used to determine trace zinc ions in environmental water samples and soil.

[0078] Determination of zinc ion content in the sample of table 2 (n=3)

[0079]

[0080] The present invention uses TritonX-100 and SDS as a mixed surfactant, 1-(2-pyridylazo)-2-naphthol (PAN) as a metal ion complexing agent, and combines cloud point extraction with ultraviolet spectrophotometry to determine trace amounts of Zn 2+ . The effects of different reaction conditions such as pH value, complexing agent dosage, surfactant concentrati...

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Abstract

The invention discloses a method for detecting trace zinc ions by using cloud point extraction and ultraviolet-visible spectrophotometry. The method comprises the following steps: adding a sample to be detected to a cloud point extraction system containing an NH4Cl-NH3.H2O buffer solution with pH of 8.5, 1-(2-pyridylazo)-2-naphthol, TritonX-100, SDS (Sodium Dodecyl Sulfonate) and saturated sodium chloride NaCl; uniformly mixing and putting the mixture in a refrigerator to balance; and separating two phases by centrifuging, collecting a lower phase, diluting by using absolute ethyl alcohol and detecting the content of the zinc ions by ultraviolet-visible spectrophotometry, wherein the absorption wavelength of the spectrophotometry is 549nm. The method can be used for detecting trace zinc ions in environmental water samples and soil; the detection limit of the zinc ions is 3.3ng.mL<-1>; the enrichment factor is 38; the relative standard deviation is 2.8%; the adding standard recovery rate is 97.7%-101.5%.

Description

technical field [0001] The invention relates to a method for measuring trace metal ions, in particular to a method for measuring trace zinc ions by cloud point extraction-ultraviolet-visible spectrophotometry. Background technique [0002] Zinc plays an important role in the production of enzymes. Zinc is important in the function of the immune system, digestive system, and nervous system, and is also important in some foods. A large number of zinc metalloenzymes are used in all metabolisms. Zinc deficiency in the body can lead to skin diseases, slow wound recovery, decreased sense of taste and smell, susceptibility to infection, reduced fertility, slow growth of children, lethargy, loss of appetite and easy hair loss. Zinc is highly resistant to corrosion and is widely used as a protective coating in different products. The U.S. Environmental Protection Agency (EPA) has announced that the zinc content in drinking water should not exceed 5 mg L -1 . [0003] Determinati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
Inventor 杨秀培杨晓萃王修海谭志敬李固贾智慧
Owner CHINA WEST NORMAL UNIVERSITY
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