Application of oridonin powder aerosol in treatment of acute lung injury

A technology of oridonin and acute lung injury, which is applied in the field of medicine and can solve the problems that there is no specific drug for acute lung injury.

Inactive Publication Date: 2014-12-24
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no clinically effective drug for acute lung injury

Method used

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  • Application of oridonin powder aerosol in treatment of acute lung injury
  • Application of oridonin powder aerosol in treatment of acute lung injury
  • Application of oridonin powder aerosol in treatment of acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1. Preparation of oridonin nanosuspension powder spray

[0042] Take 100mg of Rubescensine A and 300mg of soybean lecithin, put them in a 50ml centrifuge tube, add 3ml of absolute ethanol, and ultrasonically dissolve them completely for 3 minutes, and use it as the organic phase; take 1.2g of mannitol and dissolve it in 30mL of pure water as the water phase. The aqueous phase was slowly injected into the organic phase in a vortex state to prepare a milky white suspension ( figure 1 ). The oridonin A nanosuspension was freeze-dried to obtain oridonin A powder spray ( figure 2 ). Get a small amount of oridonin A powder spray and disperse it in water for injection to get a white suspension ( image 3 ). Oridonin suspension before freeze-drying ( Figure 4 ) and the suspension after redispersion of oridonin powder aerosol ( Figure 5 ) are in regular spherical shape, the particle size of the former is about 100-150nm, the polydispersity coefficient is 0....

Embodiment 2

[0043] Embodiment 2. Preparation of oridonin nano-emulsion powder spray

[0044] Take 50 mg of Rubescensin A and dissolve it in 5 ml of ethyl oleate / tetrahydrofuran mixed solvent, add appropriate amount of polyoxyethylene castor oil and monoglyceride, heat and stir to form a solution, add about 4 ml of water under high-speed stirring conditions, and continue stirring , to obtain a translucent dispersion liquid. The results of particle size measurement showed that most of the particles were below 120nm, and the oridonin nanoemulsion was obtained. Take 100mg of mannitol and add 4ml of oridonin A nanoemulsion to dissolve, freeze-dry in a lyophilizer to obtain oridonin A nanoemulsion powder with good fluidity. Take 100 mg of Rubescensin A nanoemulsion powder and mix with 100 mg of Inhalac230 type lactose produced by Meggle Company to obtain Oridonin A nanoemulsion dry powder inhaler.

Embodiment 3

[0045] Embodiment 3. Oridonin A liposome powder spray preparation

[0046] Take 100mg of Rubescensin A, 320mg of soybean lecithin, and 30mg of cholesterol in an eggplant-shaped bottle, add 5ml of tetrahydrofuran to dissolve it completely, and rotatively evaporate in a water bath at 35°C to remove the organic solvent, forming a thin uniform film on the inner wall of the eggplant-shaped bottle . Add 2.26g of lactose in 200ml of pH 5.0 phosphate buffer solution into an eggplant-shaped bottle, place at 37°C and shake at a constant temperature of 100 rpm for 1 hour to fully hydrate it. After taking it out, it was ultrasonically dispersed for 10 minutes to obtain oridonin A liposome suspension. Spray-drying the oridonin A liposome to obtain the oridonin A liposome dry powder inhaler. The spray drying parameters are as follows: air inlet temperature: 100°C, air outlet temperature: 50°C, fan power: 55Hz, peristaltic pump speed: 280mL / h, firing pin interval: 2 seconds.

[0047] A ...

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Abstract

The invention discloses application of oridonin powder aerosol in treatment of acute lung injury. The oridonin powder aerosol is prepared by an oridonin nano-drug delivery system, wherein the dosage form of the nano-drug delivery system is selected from lipidosome, nanoparticles, nanosuspension, nanoemulsion and micro-emulsion. The medicines in the oridonin powder aerosol are stable, easily enter the deep parts of lung tissues, are positioned in a targeted mode and are convenient to carry and use, and the treatment of acute lung injury is promoted. The oridonin powder aerosol is used for treating the acute lung injury caused by infection, shock, smoking, trauma, toxin poisoning, inhalation of irritant gases, radiation, high oxygen and low oxygen, wherein the inhaled irritant gases comprise phosgene, diphosgene, triphosgene, chlorine, nitric oxides, formaldehyde, dimethyl sulfate, hydrogen chloride, hydrogen bromide, hydrogen fluoride, ammonia, ozone and sulfur dioxide.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of oridonin A powder in the treatment of acute lung injury. Background technique [0002] Oridonin A, also known as Oridonin, Yanming Cao Ning, is the main active ingredient of Oridonin. Oridonin A is a 7,20-epoxy-type ent-kaurane diterpenoid compound, which is a light yellow needle-like crystal with a very bitter taste, slightly soluble in water, soluble in methanol, ethyl acetate, acetone and other organic solvents. Modern pharmacology and toxicology studies have shown that Rubescensine A has obvious inhibitory or killing effects on a variety of tumor cells, can enhance immunity, anti-oxidation, anti-bacterial, anti-inflammatory, anti-mutation, and has less toxic and side effects. Because oridonin is insoluble in water, the absorption rate is low, and the biological half-life in vivo is short, which limits the clinical application of oridonin. [0003] The advantages ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/12A61K31/352A61P11/00
Inventor 金义光杜丽娜李淼向晓琴
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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