The invention relates to a powder aerosol moving type oro-nasal inhalation and contamination device which comprises an exposure chamber, an experimental animal fixator, an air compressor, a powder diffuser, a diffusion airflow pipe, a vibration device, a powder aerosol sample injection pipe, a hedging type blending chamber, a hedging dilution airflow pipe, a fan and a microprocessor control unit. The powder diffuser is used for solving the problem of quantitative spraying and diffusion of solid test substance powder; the powder diffuser is connected with the hedging type blending chamber through the powder aerosol sample injection pipe, the hedging type blending chamber is fixed to the top of the exposure chamber and communicated with the inside of the exposure chamber, the exposure chamber is provided with an exposure chamber port, the front end of the experimental animal fixator is connected with the exposure chamber through the exposure chamber port, and the microprocessor control unit is used for regulating operating parameters of the contamination device. The powder aerosol moving type oro-nasal inhalation and contamination device simulates a powder aerosol exposure mode in an actual environment, the concentration of test substances is distributed evenly, an exposure state is stable, experimental result repeatability is good, and the powder aerosol moving type oro-nasal inhalation and contamination device is fast and convenient to operate and wide in applied range.