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A kind of extraction and separation method of dendrobium nobile polysaccharide and total alkali

A Dendrobium nobile, separation method technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve the problems of long extraction cycle, low utilization rate of raw materials, high equipment requirements, etc. , to achieve the effect of simple and easy preparation process, improving quality and safety, and retaining biological activity

Inactive Publication Date: 2017-02-08
ANHUI UNIVERSITY
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  • Description
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Problems solved by technology

[0006] At present, in the extraction of Dendrobium nobile polysaccharides and total biological alkaloids, most of them adopt the method of separate extraction, which will make the extraction cycle long, the production efficiency is low, it is not conducive to large-scale production, and the utilization rate of raw materials is low. The phenomenon
CN101407557 discloses the preparation and application of a polysaccharide extract of Dendrobium nobile. The method realizes the degreasing of petroleum ether, high-temperature extraction, ethanol precipitation, Sevage reagent for protein removal, monosaccharide and oligosaccharide removal, and gel column purification. The extraction of Dendrobium nobile polysaccharides, although the polysaccharide content of Dendrobium nobile obtained by this method is high, but the yield is low, high temperature may partially destroy the biological activity of Dendrobium polysaccharides, the production is complicated, the cycle is long, the cost is high, and the pollution is serious. In industrial production Not easy to achieve
CN101735231A The method for extracting dendrobium alkaloids by high-concentration ethanol high-temperature extraction requires high equipment requirements, high energy consumption, low efficiency, and uses chloroform and ether for extraction, which has high toxicity and serious pollution

Method used

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  • A kind of extraction and separation method of dendrobium nobile polysaccharide and total alkali
  • A kind of extraction and separation method of dendrobium nobile polysaccharide and total alkali
  • A kind of extraction and separation method of dendrobium nobile polysaccharide and total alkali

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The extraction and separation method of Dendrobium nobile polysaccharide and total alkaloids in the present embodiment is as follows:

[0060] 1) Sample pretreatment

[0061] Wash and dry 1 kg of Dendrobium nobile stem stalks with uniform thickness and bright yellow color, and after crushing, pass through a 60-mesh sieve to obtain Dendrobium nobile powder;

[0062] 2) Enzymolysis

[0063] Add the Dendrobium nobile powder prepared in step 1) to 3 times the mass of water, then add 0.1% biological enzyme of the Dendrobium nobile powder, adjust the pH value to 4.0 with citric acid, and enzymatically hydrolyze at 55°C for 10 hours;

[0064] The biological enzyme is composed of cellulase, pectinase and protease, wherein the mass ratio of cellulase, pectinase and protease is 10:40:50.

[0065] 3) High-pressure homogeneous extraction

[0066] Add water to the solid-liquid mixture obtained after enzymolysis so that the water content in the solid-liquid mixture is 10 times the...

Embodiment 2

[0074] The extraction and separation method of Dendrobium nobile polysaccharide and total alkaloids in the present embodiment is as follows:

[0075] 1) Sample pretreatment

[0076] Wash and dry 3 kg of Dendrobium nobile stalks with uniform thickness and bright yellow color, and after crushing, pass through an 80-mesh sieve to obtain Dendrobium nobile powder;

[0077] 2) Enzymolysis

[0078] Add the Dendrobium nobile powder prepared in step 1) to 5 times the mass of water, then add biological enzymes with a mass of 1% of the Dendrobium nobile powder, adjust the pH value to 5.0 with citric acid, and enzymatically hydrolyze at 35°C for 3 hours;

[0079] The biological enzyme is composed of cellulase, pectinase and protease, wherein the mass ratio of cellulase, pectinase and protease is 20:40:40.

[0080] 3) High-pressure homogeneous extraction

[0081] Add water to the solid-liquid mixture obtained after enzymolysis so that the water content in the solid-liquid mixture is 30 ...

Embodiment 3

[0089] The extraction and separation method of Dendrobium nobile polysaccharide and total alkaloids in the present embodiment is as follows:

[0090] 1) Sample pretreatment

[0091] Wash and dry 1 kg of Dendrobium nobile stem stalks with uniform thickness and bright yellow color, and after crushing, pass through a 60-mesh sieve to obtain Dendrobium nobile powder;

[0092] 2) Enzymolysis

[0093] Add the Dendrobium nobile powder prepared in step 1) to 4 times the mass of water, then add biological enzymes with a mass of 1% of the Dendrobium nobile powder, adjust the pH value to 4.0 with citric acid, and perform enzymatic hydrolysis at 45°C for 8 hours;

[0094] The biological enzyme is composed of cellulase, pectinase and protease, wherein the mass ratio of cellulase, pectinase and protease is 15:35:50.

[0095] 3) High-pressure homogeneous extraction

[0096] Add water to the solid-liquid mixture obtained after enzymolysis so that the water content in the solid-liquid mixtu...

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Abstract

The invention discloses a method for extracting and separating polysaccharides and total alkaloids of Dendrobium nobile, which uses medicinal material Dendrobium nobile as a raw material, and successively undergoes crushing, sieving, enzymatic hydrolysis, high-pressure homogenization, flocculation, ultrafiltration membrane separation, and freeze-drying and other processes to maximize the extraction of Dendrobium nobile polysaccharides and total alkaloids, avoid the use of organic reagents, and at the same time facilitate the industrialization and large-scale production of Dendrobium nobile deep processing technology. The present invention uses high-pressure homogenization combined with ultrafiltration technology in the process of extracting Dendrobium nobile polysaccharides and total alkaloids without using any organic reagents to protect the biological activity of Dendrobium nobile polysaccharides to the greatest extent, and simultaneously extracts total alkaloids from Dendrobium nobile Raw materials can be fully utilized. The polysaccharide content of the dendrobium nobile obtained by the method of the invention is more than 94%, and the total alkali content of the dendrobium is more than 90%.

Description

[0001] 1. Technical field [0002] The invention relates to an extraction and separation method, in particular to an extraction and separation method of dendrobium nobile polysaccharide and total alkaloids, belonging to the field of deep processing of agricultural products. [0003] 2. Background technology [0004] Dendrobium belongs to Orchidaceae and is a perennial herb. There are more than 1,500 species found in the world, and 78 species are known in my country. Dendrobium nobile is a traditional Chinese medicinal material included in the 2010 edition of the Chinese Pharmacopoeia. It is the highest content of total alkaloids in many Dendrobium plants and the only Chinese herbal species containing dendrobium. Its chemical composition is relatively complex, mainly alkaloids and polysaccharides substance. Dendrobium can relieve pain, relieve fever, reduce heart rate and blood pressure, slow down breathing, and detoxify barbiturates; Dendrobium nobile polysaccharide has anti-o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K36/8984
Inventor 陈彦王亚徐利郭文强陆永明李绍飞
Owner ANHUI UNIVERSITY
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