Method for high-efficiency extraction of nutrient substances in undaria pinnatifida sporophyll

A technology of wakame spore leaves and nutrients, which is applied in the directions of biofuels, fertilizer mixtures, applications, etc., can solve the problems such as the potential advantages of humic acid not being exerted, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2015-02-11
QINGDAO BOYANDA IND TECH RES INST GENERAL PARTNERSHIP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The potential advantages of humic acid have not been exerted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Remove the silt from the undaria sporophyll raw material, dry it at 60°C until the water content is below 8%, and crush it to a powder with a particle size below 1.0mm as the raw material; accurately weigh 100kg of the wakame sporophyll after drying and crushing, add Fresh drinking water with a raw material weight of 4000ml was treated with a 400W ultrasonic wave for 15 minutes; the pH was adjusted to 4 with 4mol / L hydrochloric acid, and 40% of endo-β-glucanase and exo-β-glucanase were added with 0.5% of raw material weight. 0.4% cellulase composed of 35%, β-glucose alfalfa enzyme 25%, enzymolysis at 50°C for 3 hours; add 0.3% protease composed of 55% neutral protease, 45% papain, pectin lyase 50% %, 0.2% pectinase composed of 50% pectin esterase, continue enzymolysis for 4 hours, stir, and centrifuge after enzymolysis;

[0027] Add 1300kg 2% Na to the wakame sporophyll after the above centrifugation 2 CO 3 Digest the solution in a water bath at 55°C for 2 hours; then...

Embodiment 2

[0031] Remove the silt from the undaria sporophyll raw material, dry it at 70°C until the moisture content is below 10%, and crush it to a powder with a particle size below 1.5mm as the raw material; accurately weigh 100kg of the wakame sporophyll after drying and crushing, add Fresh drinking water with a raw material weight of 4000ml was treated with a 600W ultrasonic wave for 20 minutes; the pH was adjusted to 6 with 8mol / L hydrochloric acid, and 2.0% of the raw material weight was added with 40% of endo-β-glucanase and exo-β-glucanase 0.6% cellulase composed of 35%, β-glucose alfalfa enzyme 25%, enzymatic hydrolysis at 60°C for 4 hours; add 0.4% protease composed of 55% neutral protease, 45% papain, pectin lyase 50% %, 0.3% pectinase composed of 50% pectin esterase, continue enzymolysis for 5h, stir, centrifuge after enzymolysis;

[0032] Add 1500kg of 3% Na to the wakame sporophyll after the above centrifugation 2 CO 3 Digest the solution in a water bath at 65°C for 3 ho...

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Abstract

The invention discloses a method for high-efficiency extraction of nutrient substances in undaria pinnatifida sporophyll, and is characterized in that the method comprises the following steps: (1) raw material pretreatment, (2) ultrasonic treatment, (3) cellulose enzymolysis, (4) proteolysis, (5) brine digestion, (6) dilution and filtration, (7) decoloring, (8) CaCl2 solution treatment, (9) ion exchange, (10) preparation of sodium alginate, (11) preparation of ethanol, and (12) preparation of an undaria pinnatifida sporophyll organic fertilizer. A composite enzyme is prepared from protease, pectinase and cellulase by a scientific ratio, and raw material is pretreated, so as to improve the extraction rate of sodium alginate; at the same time, fuel ethanol is prepared by using cellulose-rich undaria pinnatifida sporophyll processing waste; Chinese humic acid resources are fully developed and utilized in the processing process, the organic fertilizer rich in humic acid and macronutrients is produced with water as a solvent, and the high-valued comprehensive utilization of the undaria pinnatifida sporophyll is realized.

Description

technical field [0001] The invention relates to the field of utilization of wakame spore leaves, in particular to a method for efficiently extracting nutrients from wakame spore leaves. Background technique [0002] At present, the world is facing the huge pressure of energy shortage and environmental pollution, and the development of clean and renewable energy has become an important means to solve the crisis of petrochemical resources and energy. Bioethanol has been widely concerned as a clean fuel and gasoline additive. At present, the preparation of fuel ethanol in my country mainly focuses on terrestrial raw materials such as straw, bagasse, wood chips and cassava, but there are few studies on the preparation of bioethanol from marine biological resources, and the technical research on the development of wakame spore leaf biomass energy is relatively backward. [0003] The ocean area is huge, and there are rich resources of wakame sporophyll, which have various types, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C08B37/04C12P7/10
CPCY02E50/10
Inventor 朱凤
Owner QINGDAO BOYANDA IND TECH RES INST GENERAL PARTNERSHIP
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