Nano carrier prepared from marine sulfated polysaccharide, nano complex and application of nano carrier

A nanocomposite, sulfated polysaccharide technology, applied in the application field to achieve the effects of improving stability, efficient utilization, and abundant sources

Active Publication Date: 2015-03-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patents such as Jin Rongzhen (publication number CN101910256A) disclosed for the first time the method of utilizing synthetic polymers to prepare carotenoid double-layer capsules, which improved the stability and water dispersibility of fat-soluble

Method used

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  • Nano carrier prepared from marine sulfated polysaccharide, nano complex and application of nano carrier
  • Nano carrier prepared from marine sulfated polysaccharide, nano complex and application of nano carrier
  • Nano carrier prepared from marine sulfated polysaccharide, nano complex and application of nano carrier

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Effect test

Embodiment 1

[0067] The preparation of embodiment 1 sea cucumber chondroitin sulfate / chitooligosaccharide nanocarrier:

[0068] Take 0.1 mg / ml sea cucumber chondroitin sulfate aqueous solution, mix it with 0.1 mg / ml chitosan oligosaccharide solution, and stir at high speed for 30 minutes to obtain sea cucumber chondroitin sulfate / chitooligosaccharide nanocarrier. Then, the Tyndall phenomenon was observed on the prepared nanocarrier suspension as follows: figure 1 shown. from figure 1 It can be seen that when a beam of light passes through the prepared colloidal solution, a bright "path" can be observed in the colloid from the vertical direction of the incident light, indicating that sea cucumber chondroitin sulfate and chitosan oligosaccharides are successfully compounded to form nanocarriers .

Embodiment 2

[0069] The preparation of embodiment 2 fucoxanthin / carrageenan / chitosan nanoparticles:

[0070] Take 0.1 mg / ml chitosan dilute hydrochloric acid solution, mix it with 0.02 mg / ml ethanol solution of fucoxanthin and 0.1 mg / ml carrageenan solution in turn, stir in the dark for 10 minutes, and then spin evaporate to remove ethanol to obtain entrapped rock Chitosan-carrageenan nanocomposite suspension of alcoxanthin; transfer the nanocomposite suspension to a clean glass container, add an equal volume of deionized water and 1% glucose solution in sequence, and freeze the mixed solution at -50°C After 24 hours, use a vacuum freeze dryer to freeze-dry for 24 hours to obtain fucoxanthin / carrageenan / chitosan nanoparticles. The absorption spectrum of the nanocomposite suspension was detected by an ultraviolet photometer, such as figure 2 The light absorption at 450 nm proves that the nanocomposite has successfully encapsulated fucoxanthin, and the encapsulation efficiency of fucoxanth...

Embodiment 3

[0071] The preparation of embodiment 3 astaxanthin / fucoidan sulfate / chitosan nanoparticles:

[0072] Take 0.2mg / ml chitosan dilute hydrochloric acid solution, mix it with 0.03mg / ml ethanol solution of astaxanthin and 0.07mg / ml fucoidan sulfate solution successively, stir in the dark for 10 minutes, and then remove ethanol by rotary evaporation. The astaxanthin-loaded chitosan-fucoidan sulfate nanocomposite is obtained, that is, the astaxanthin / fucoidan sulfate / chitosan nanocomposite. image 3 It is the ultraviolet spectrogram of the nanocomposite. The light absorption at 390nm proves that the astaxanthin contained in the nanocomposite exists in the form of H-type vesicles, and the astaxanthin content in the nanosystem can be calculated to be 20 μg / ml.

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Abstract

The invention discloses a nano carrier prepared from marine sulfated polysaccharide, a nano complex, and an application of the safe and nontoxic nano carrier with specific activity of the marine sulfated polysaccharide. According to the technique, the nano carrier is prepared from marine sulfated polysaccharide with certain biological activity as an edible and safe water-soluble biological macromolecular material by using intermolecular crosslinking and compounding of the marine sulfated polysaccharide and chitosan and derivatives of the chitosan; a hydrophobic microdomain formed by convolving between polysaccharide molecules is beneficial to adsorption and aggregation of fat-soluble active substances; entrapment and dispersion of a water-soluble nano carrier on the fat-soluble active substances are achieved; and the nano complex with good biological activity is formed. The prepared nano complex is beneficial to transportation and absorption of the active substances in vivo; and the bioavailability of the active substances is improved.

Description

technical field [0001] The invention relates to the fields of biology, medicine, food and materials, in particular to a nano-carrier and a nano-composite prepared from marine sulfated polysaccharides and its application as an edible nano-carrier. Background technique [0002] Sulfated polysaccharides are an important class of active substances, especially marine sulfated polysaccharides isolated from various marine organisms (animals, plants, microorganisms), because they generally have the characteristics of high sulfation, they are the best choice for the development of antiviral and anticoagulant preparations. important resource. In addition, marine sulfated polysaccharides also have some special biological activities due to their special molecular structure (such as molecular weight, monosaccharide type and glycosidic bond composition, position and degree of substitution of sulfate groups, etc.). For example, low-molecular-weight fucoidan sulfate (8000-10000 Daltons) ex...

Claims

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Application Information

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IPC IPC(8): A61K47/36A23L1/29A23P1/02A23L33/00
CPCA61K9/14A61K47/36
Inventor 李敬薛长湖常耀光李兆杰梁兴国
Owner OCEAN UNIV OF CHINA
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