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Broad-spectrum salmonella bacteriophage biological bactericide and application thereof

A bacteriophage and salmonella technology, applied in the field of bioengineering, can solve problems such as multi-drug resistance, hinder the development of poultry industry, threaten the health of humans and animals, and achieve the effect of wide source and low cost

Active Publication Date: 2015-04-01
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] salmonella (Salmonella) It is a serious pathogenic bacteria in livestock and poultry breeding, and it is also an important zoonotic pathogen that causes food poisoning. Globally, the problem of Salmonella infection and pollution has attracted great attention from the poultry industry, food safety and public health circles. It not only threatens human and animal health, but also seriously hinders the development of poultry industry, becoming an important focus of animal husbandry production and food safety. At present, due to the widespread use and abuse of antibiotics, the multidrug resistance of the entire Salmonella genus has also changed from the 1990s to 20th century. 20%-30% in the 1990s increased to 70% at the beginning of this century. With the further intensification of antibiotic resistance, Salmonella has produced serious multi-drug resistance in the long-term evolution process. , there is no effective and safe measure

Method used

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  • Broad-spectrum salmonella bacteriophage biological bactericide and application thereof
  • Broad-spectrum salmonella bacteriophage biological bactericide and application thereof
  • Broad-spectrum salmonella bacteriophage biological bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation, amplification and purification of Salmonella phage

[0024] The fecal and urine sewage samples from the chicken farm in this example came from a chicken farm in Nantong City, Jiangsu Province; the host bacterium was Salmonella Enteritidis SM-54-KDE preserved in this laboratory, which was isolated from sick chickens in a chicken farm in Jinan City.

[0025] Phage isolation: Collect fecal and urine sewage samples from chicken farms, filter them through double-layer filter paper, centrifuge at 12000×g for 20 minutes, and then filter the supernatant with 0.45 μm and 0.22 μm filter membranes respectively; take 10 mL of the filtrate, add 0.5 mL Overnight cultured host bacteria (with a bacterial content of 10 9 cfu / mL), then add 20mL 4×LB medium, place it at 37°C and incubate for 12-16h, the next day, take the above culture and centrifuge at 12000×g for 10min, take the supernatant and add 0.3% of the volume of the supernatant in chloroform Stand...

Embodiment 2

[0030] Example 2 Isolation, amplification and purification of Salmonella typhimurium phage

[0031] The feces and urine sewage samples from chicken farms in this example came from a chicken farm in Nantong City, Jiangsu Province; the host bacteria was Salmonella typhimurium HNER178, which was isolated from a sick and dead chicken in a farm in Jiangxi Province, and was preserved in our laboratory.

[0032] The isolation, amplification and purification of Salmonella typhimurium phage, except that the host bacteria is Salmonella typhimurium HNER178, the rest of the steps are the same as in Example 1, and the titer of the purified Salmonella typhimurium phage is ≥10 8 pfu / mL.

[0033] The applicant self-named the Salmonella typhimurium phage as JX178, and its microbiological characteristics are as follows: figure 2 As shown, the phage has a polyhedral head structure and a long tail, and should belong to the longtail virus family, and the phage belongs to the lytic pha...

Embodiment 3

[0035] Example 3 Isolation, Amplification and Purification of Salmonella Delphi Phage

[0036] In this example, the sewage sample came from a farmer's market in Jiangsu Province; the host bacteria was Salmonella delbei HNER067-2, which was isolated from a farmer's market in Jiangxi Province and preserved in our laboratory.

[0037] For the isolation, amplification and purification of Salmonella Delphi phage, except that the host bacterium is Salmonella Delphi, the other operating steps are the same as those in Example 1, and the titer of the purified Salmonella Delphi phage is ≥10 8 pfu / mL. The applicant self-named the Salmonella phage JX067-2, and its microbiological characteristics are as follows: image 3 As shown, the phage has a polyhedral head structure and a long tail, and should belong to the longtail virus family, and the phage belongs to the lytic phage.

[0038] The applicant deposited the purified Salmonella delphi phage JX067-2 in the China Center for T...

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Abstract

The invention discloses a broad-spectrum salmonella bacteriophage biological bactericide and an application thereof. The biological bactericide comprises salmonella bacteriophage and an auxiliary agent, wherein the salmonella bacteriophage comprises at least more than three of salmonella bacteriophage PSA-6, pullorosis salmonella bacteriophage PSPu-4-116, salmonella enteritidis phage, salmonella typhimurium phage, salmonlla derby phage or London salmonella bacteriophage, wherein the preservation number of the salmonella enteritidis phage is CCTCC NO:M2014429; the preservation number of the salmonella typhimurium phage is CCTCC NO:M2014433; the preservation number of the salmonlla derby phage is CCTCC NO:M2014431; and the preservation number of the London salmonella bacteriophage is CCTCC NO:M2014432. The biological bactericide is compounded of a plurality of lytic salmonella bacteriophages to serve as an effective germicidal ingredient, so that the sterilization range is greatly expanded and the bactericidal activity is increased.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a broad-spectrum Salmonella phage biological bactericide and its application. Background technique [0002] salmonella (Salmonella) It is a serious pathogenic bacteria in livestock and poultry breeding, and it is also an important zoonotic pathogen that causes food poisoning. Globally, the problem of Salmonella infection and pollution has attracted great attention from the poultry industry, food safety and public health circles. It not only threatens human and animal health, but also seriously hinders the development of poultry industry, becoming an important focus of animal husbandry production and food safety. At present, due to the widespread use and abuse of antibiotics, the multidrug resistance of the entire Salmonella genus has also changed from the 1990s to 20th century. 20%-30% in the 1990s increased to 70% at the beginning of this century. With the further intensificati...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01P1/00A23K1/16A61K35/76A61P31/12C12R1/92
CPCY02A50/30
Inventor 包红朵王冉张辉周艳张莉莉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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