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A pumpkin yellow pigment yeast product and preparation method thereof

A yellow pigment and pumpkin technology, applied in the field of food processing, can solve the problems of unreported research and its application, non-environmental protection, energy consumption, low extraction rate, etc., achieve rich and balanced ingredients, realize comprehensive utilization, and reduce accumulation Effect

Active Publication Date: 2018-06-05
四川安益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing pumpkin pigment extraction methods all involve the use of organic solvents (ethanol, acetone, ether, etc.), the extraction process requires high temperature, and the extraction and recovery of the extract solution are also required. The disadvantages are that it is not environmentally friendly and requires energy consumption. High, low extraction rate, easy to cause environmental pollution
[0006] In the prior art, there is no literature report on the technical scheme of directly releasing pumpkin pigment and the preparation of biological products through microbial fermentation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Fermentation and extraction of pumpkin pigment:

[0053] Yeast slope and seed medium composition (g / L): glucose 25, peptone 15, yeast extract 8, pH 6.0 (the same below). Seed cultivation to OD 600 = 5. In a 30-liter fermentation tank, add 12 liters of fermentation medium, the composition (g / L) is: glucose 40, (NH 4 ) 2 SO 4 4. KH 2 PO 4 3. MgSO 4 0.4, FeSO 4 0.02, MnSO 4 0.01, yeast extract 6, pH 5.2. 5% transplantation, access to 600mL of Saccharomyces cerevisiae seed liquid (OD 600 =5), the stirring speed is 180rpm, and the temperature is 29±1℃. The dissolved oxygen (DO) is controlled at about 20%, and after 12 hours of fermentation, the yeast cells in the obtained fermentation system are 16g / L.

[0054] Add 4 kg of sterilized, enzymatically hydrolyzed pumpkin pulp to the above fermentation system, and add feed medium [composition (g / L): glucose 250, ammonium sulfate 23, ammonium dihydrogen phosphate 3.2, the same below], Keep the residual sugar level in the fermentation...

Embodiment 2

[0064] Fermentation and extraction of pumpkin pigment:

[0065] The slope and seed culture medium are the same as in Example 1. Baker's yeast seed liquid cultured to OD 600 = 8, Saccharomyces cerevisiae seed liquid is cultivated to OD 600 = 6. In a 100 liter fermenter, 36 liters of medium is added, the composition (g / L) is: glucose 45, (NH 4 ) 2 SO 4 5. KH 2 PO 4 2.5, MgSO 4 0.5, FeSO 4 0.01, MnSO 4 0.03. Yeast extract 5, pH 5.5. Add 3% of baker's yeast and Saccharomyces cerevisiae seed liquid, namely 1080 mL, stirring speed 300rpm, temperature 30±1℃. Aeration rate of 0.5VVM, after 16 hours of fermentation, 15 kg of sterilized pumpkin pulp is added to the above fermentation system, and the medium (the composition is the same as in Example 1) is added to keep the residual sugar level in the fermentation broth not less than 1.5% , Continue to ferment for 45 hours, increase the stirring speed to 420rpm, adjust the ventilation to keep the dissolved oxygen greater than 15%. After t...

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PUM

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Abstract

The present invention provides a pumpkin yellow pigment yeast product and a preparation method thereof. The invention is characterized in that a fermentation method is used for the preparation of a biological product containing pumpkin yellow pigment and food-yeast hydrolysate. The method comprises the specific steps of: (1) cultivating the food-yeast by fermentation to obtain a fermentation system; (2) adding a pretreated pumpkin pulp in the fermentation system, and continuing to ferment; (3) conducting solid-liquid separation on the fermentation product obtained in the step (2) to obtain a pumpkin pigment clarified liquid; (4) adding purified water to the solid separated from the step (3) to resuspend the yeast mycelium to obtain a yeast enzymolysis liquid; and (5) concentrating, merging and drying the yeast enzymolysis liquid to obtain a solid powder composite pumpkin pigment yeast biological product. The preparation process is simple and pollution-free, the pigment release degree of yellow pigment is high, and the prepared product has high edible security and rich nutrition.

Description

Technical field [0001] The invention belongs to the field of food processing, and more specifically relates to a composite product of pumpkin pigment and edible yeast and a preparation method thereof. Background technique [0002] Pumpkin pulp is rich in polyphenolic flavonoids, such as α-carotene, β-carotene, γ-carotene, and lutein. β-carotene can be converted into vitamin A at any time in the human body. Lutein and zeaxanthin can protect the retina of the eye, and thus play a key role in the prevention and treatment of degenerative eye diseases. At the same time, pumpkin is also rich in dietary fiber, which plays an important role in the health of the digestive system and rectum. At the same time, dietary fiber is also beneficial for maintaining weight and diabetes. Pumpkin seeds are rich in strong antioxidants, minerals and omega-3 fatty acids. It also contains elements such as magnesium, zinc, copper, amino acids, manganese, and selenium. The yellow pigment contained the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12N1/18C12N1/16A23L33/14A23L33/00A23L19/00C12R1/865C12R1/645
Inventor 叶春江姜均
Owner 四川安益生物科技有限公司
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