A pumpkin yellow pigment yeast product and preparation method thereof
A yellow pigment and pumpkin technology, applied in the field of food processing, can solve the problems of unreported research and its application, non-environmental protection, energy consumption, low extraction rate, etc., achieve rich and balanced ingredients, realize comprehensive utilization, and reduce accumulation Effect
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Embodiment 1
[0052] Fermentation and extraction of pumpkin pigment:
[0053] Yeast slope and seed medium composition (g / L): glucose 25, peptone 15, yeast extract 8, pH 6.0 (the same below). Seed cultivation to OD 600 = 5. In a 30-liter fermentation tank, add 12 liters of fermentation medium, the composition (g / L) is: glucose 40, (NH 4 ) 2 SO 4 4. KH 2 PO 4 3. MgSO 4 0.4, FeSO 4 0.02, MnSO 4 0.01, yeast extract 6, pH 5.2. 5% transplantation, access to 600mL of Saccharomyces cerevisiae seed liquid (OD 600 =5), the stirring speed is 180rpm, and the temperature is 29±1℃. The dissolved oxygen (DO) is controlled at about 20%, and after 12 hours of fermentation, the yeast cells in the obtained fermentation system are 16g / L.
[0054] Add 4 kg of sterilized, enzymatically hydrolyzed pumpkin pulp to the above fermentation system, and add feed medium [composition (g / L): glucose 250, ammonium sulfate 23, ammonium dihydrogen phosphate 3.2, the same below], Keep the residual sugar level in the fermentation...
Embodiment 2
[0064] Fermentation and extraction of pumpkin pigment:
[0065] The slope and seed culture medium are the same as in Example 1. Baker's yeast seed liquid cultured to OD 600 = 8, Saccharomyces cerevisiae seed liquid is cultivated to OD 600 = 6. In a 100 liter fermenter, 36 liters of medium is added, the composition (g / L) is: glucose 45, (NH 4 ) 2 SO 4 5. KH 2 PO 4 2.5, MgSO 4 0.5, FeSO 4 0.01, MnSO 4 0.03. Yeast extract 5, pH 5.5. Add 3% of baker's yeast and Saccharomyces cerevisiae seed liquid, namely 1080 mL, stirring speed 300rpm, temperature 30±1℃. Aeration rate of 0.5VVM, after 16 hours of fermentation, 15 kg of sterilized pumpkin pulp is added to the above fermentation system, and the medium (the composition is the same as in Example 1) is added to keep the residual sugar level in the fermentation broth not less than 1.5% , Continue to ferment for 45 hours, increase the stirring speed to 420rpm, adjust the ventilation to keep the dissolved oxygen greater than 15%. After t...
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