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Preparation method of plant growth factor 4,5',7-trihydroxyisoflavone supported porous biocomposite scaffold

A technology of composite materials and porous scaffolds, applied in medical science, prostheses, etc., can solve the problems of large release amount, uneven release speed, and inability to achieve long-term effects

Inactive Publication Date: 2015-07-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method releases a large amount of active ingredients at the initial stage of stent implantation, but in the later stage of stent degradation, there is no release of active ingredients, and the release rate is extremely uneven, which cannot achieve the purpose of long-term effect on the lesion before the stent is completely degraded.

Method used

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  • Preparation method of plant growth factor 4,5',7-trihydroxyisoflavone supported porous biocomposite scaffold
  • Preparation method of plant growth factor 4,5',7-trihydroxyisoflavone supported porous biocomposite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The nano-calcium-deficient apatite-polyamino acid copolymer composite (n-CDHA-MAC) particles with a particle size of 3-5mm and an apatite content of 40wt% were mixed with foaming agents calcium sulfate dihydrate (CS) and Plant-derived 5,4′,7-trihydroxyisoflavone compound growth factor (Ge), mixed according to n-CDHA / MAC:CS:Ge=90:10:1 (mass), at an injection temperature of 175°C, injection Under the conditions of pressure 70Mpa, injection speed 80%, mold temperature 60°C, and single-mode injection volume of 40mm, the composite porous scaffold Ge-PCMS (product 1) of the present invention loaded with growth factor Ge is obtained, and the porosity is 81%. . The Ge release test was carried out in phosphate buffer solution, and the amount of Ge released by the product 1 of this example within one week was about 0.0001 g. Using the PCMS without Ge growth factor prepared by the same method as the control, the composite culture of the scaffold and MG-63 cells was cultured for 1...

Embodiment 2

[0033] The nano-calcium-deficient apatite-polyamino acid copolymer composite material particles with a particle size of 3-5mm and an apatite content of 40wt%, foaming agents CS and Ge, according to n-CDHA / MAC: CS: Ge=90 : 10:5 (mass) mixed evenly, under the conditions of injection temperature 175°C, injection pressure 70Mpa, injection speed 80%, mold temperature 60°C, and single-mode injection volume of 40mm, the composite porous scaffold Ge of the present invention was obtained. PCMS (product 2), porosity 80.4%. The Ge release test was carried out in phosphate buffer solution, and the amount of Ge released by the product 2 of this example within one week was about 0.0004 g. Using PCMS as a control, after co-cultivating scaffolds and MG-63 cells for 1, 3, 5, and 7 days, it was found that the cell proliferation degree of product 2 in this example was greater than that of PCMS, and there was a significant difference from PCMS. Cell proliferation OD values ​​such as figure 1 sh...

Embodiment 3

[0036] The particle diameter is 3~5mm and the apatite content is the nanometer calcium-deficient apatite-polyamino acid copolymer composite material particle, blowing agent CS and Ge, according to n-CDHA / MAC:CS:Ge=90 : 10:10 (mass) mixed evenly, under the conditions of 175 ℃ of injection temperature, 70Mpa of injection pressure, 80% of injection speed, 60 ℃ of mold temperature, and the single-mode injection volume of 40mm, obtain the composite material porous support Ge of the present invention- PCMS (product 3), porosity 81.7%. The Ge release test was carried out in phosphate buffer solution, and the amount of Ge released by the product 3 of this example within one week was about 0.001 g. Using PCMS as a control, the composite culture of scaffolds and MG-63 cells found that the degree of cell proliferation was greater than that of PCMS, and there was a significant difference from PCMS.

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Abstract

The invention provides a soy isoflavone supported biological composite material porous scaffold and preparation. According to the porous scaffold, a nano calcium-deficient apatite-poly-amino acid copolymer composite material porous scaffold is used as a carrier, soy isoflavone is mixed in the carrier material, particularly, plant sourced 5,4',7-genistein is used as a growth factor component, wherein the mass ratio of the growth factor and the composite material is (1-10):100. When the porous scaffold is prepared, the components and a calcium sulphate dihydrate component foaming agent which is 5-15 percent by mass of the composite material are mixed, and are subjected to injection molding in an injection manner. Experiment results indicate that the cell proliferation degree of the porous scaffold to MG-63 is remarkably superior to that of a scaffold without supporting of soy isoflavone, the porous scaffold has significant difference and has an excellent prospect in serving as a scaffold material with excellent osteogenesis promoting performance.

Description

[0001] This application is a divisional application of the patent application with filing date: June 30, 2014, application number: 201410303875.9, and invention name: biocomposite porous scaffold loaded with soybean isoflavone and its preparation. technical field [0002] The invention relates to a preparation method of a degradable nano-calcium-deficient apatite / polyamino acid copolymer composite material porous scaffold, in particular soybean isoflavone is mixed in the scaffold material as a growth factor component to promote bone cell proliferation and formation A method for preparing bone nano calcium-deficient apatite / polyamino acid copolymer composite porous scaffold. Background technique [0003] Nonunion is a common complication in fracture treatment. At present, the main method of clinical treatment is to remove fibrous tissue, etc., then implant bone graft material, change internal fixation, and apply pressure to the fracture end. Bone graft materials commonly use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/54A61L27/56
Inventor 李鸿严永刚吕国玉杨爱萍
Owner SICHUAN UNIV