Tumor-targeted hollow core-shell structure nano diagnosis-treatment agent as well as preparation method and application thereof

A core-shell structure, tumor-targeting technology, applied in anti-tumor drugs, preparations for in vivo tests, medical preparations with inactive ingredients, etc. limited and other problems, to achieve the effect of increasing the load, increasing the load, and reducing the agglomeration

Active Publication Date: 2015-07-22
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the therapeutic efficiency of these therapeutic agents is limited. The main reason is that the loading of the photosensitizer is too low. The loaded photosensitizer contains a conjug

Method used

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  • Tumor-targeted hollow core-shell structure nano diagnosis-treatment agent as well as preparation method and application thereof
  • Tumor-targeted hollow core-shell structure nano diagnosis-treatment agent as well as preparation method and application thereof
  • Tumor-targeted hollow core-shell structure nano diagnosis-treatment agent as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0057] Example 1: Preparation of nano-diagnostic and therapeutic agent loaded with zinc monocarboxyphthalocyanine (ZnPc-COOH)

[0058] (1) Preparation of hexagonal phase NaLuF based on thermal decomposition method 4 :Gd / Yb / Er nanocrystal

[0059] Weigh out sodium trifluoroacetate, lutetium trifluoroacetate, gadolinium trifluoroacetate, ytterbium trifluoroacetate and erbium trifluoroacetate at room temperature (total 2mmoL, the molar ratio of each element is 1 lutetium: 0.6 gadolinium: 3Na: 0.36 ytterbium: 0.04 Erbium) into a three-hole flask, add 6mL oleic acid, 3mL oleylamine and 3mL octadecene as solvents; heat under an inert atmosphere until the above trifluoroacetate is dissolved and then continue to raise the temperature to 300°C, react for 1 hour and then naturally cool to At room temperature, precipitate and wash to obtain NaLu(Gd)F 4 : Yb / Er nanocrystal.

[0060] NaLu(Gd)F 4 : Yb / Er is added to 6mL oleic acid and 6mL octadecene solution, sodium trifluoroacetate, lutetium tri...

Example Embodiment

[0067] Example 2: Preparation of nano diagnostic and therapeutic agent loaded with Rose Bengal (RB)

[0068] (1) Preparation of hexagonal phase NaLuF based on thermal decomposition method 4 :Gd / Yb / Er nanocrystal

[0069] Weigh out sodium trifluoroacetate, lutetium trifluoroacetate, gadolinium trifluoroacetate, ytterbium trifluoroacetate and erbium trifluoroacetate at room temperature (total 2mmoL, the molar ratio of each element is 1 lutetium: 0.6 gadolinium: 3Na: 0.36 ytterbium: 0.04 Erbium) into a three-hole flask, add 6mL oleic acid, 3mL oleylamine and 3mL octadecene as solvents; heat under an inert atmosphere until the above trifluoroacetate is dissolved and then continue to raise the temperature to 300°C, react for 1 hour and then naturally cool to At room temperature, precipitate and wash to obtain NaLu(Gd)F 4 : Yb / Er nanocrystal. NaLu(Gd)F 4 : Yb / Er is added to 6mL oleic acid and 6mL octadecene solution, sodium trifluoroacetate, lutetium trifluoroacetate, ytterbium trifluor...

Example Embodiment

[0076] Example 3: Application performance test of nano diagnostic and therapeutic agent loaded with ZnPc-COOH

[0077] (1) Detection of singlet oxygen under 980 light: such as Figure 7 As shown, the nano diagnostic and therapeutic agent prepared in Example 1 has a power of 1W / cm 2 980nm laser irradiation for 26 minutes, the generated singlet oxygen can reduce the probe 1,3-diphenylisobenzofuran (DPBF) by 93%, while other contrast materials (including ordinary silica shell and ordinary core-shell The structure) only decreased by about 50%, indicating that the nano diagnostic and therapeutic agent can generate more singlet oxygen.

[0078] (2) Detection of the effect of in vitro photodynamic therapy on human lung cancer cells (H1299): Picture 8 As shown, the nano diagnostic and therapeutic agent prepared in Example 1 was incubated with human lung cancer cells (H1299) for 4 hours and then washed with PBS for photodynamic therapy test, and the cell survival rate was calculated by MTT ...

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Abstract

The invention relates to a tumor-targeted hollow core-shell structure nano diagnosis-treatment agent and a preparation method thereof. The nano diagnosis-treatment agent comprises an upconversion nanoparticle inner core, an organic-inorganic hybrid silica shell layer and an intermediate cavity structure, can improve the load capacity of a photosensitizer and reduce the photosensitizer aggregation, and can specifically recognize cancer cells by connecting tumor-targeted molecules (such as urokinase ammonia terminal fragments) to the surface; under the irradiation of 980 nm laser, a visible light emission sensitizing photosensitizer of the nano diagnosis-treatment agent generates singlet oxygen, so that the photodynamic therapy of cancer cells can be performed; in addition, the nano diagnosis-treatment agent can be used for the upconversion luminescence and CT dual-mode imaging by utilizing the upconversion luminescence and strong X-ray absorption characteristics.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and in particular relates to a tumor-targeted nano-diagnosis and treatment agent with hollow core-shell structure and its preparation method and application. Background technique [0002] With the development of biomedicine, the multifunctional combination of diagnosis and treatment (referred to as diagnosis and treatment) has become a new trend. Diagnosis and treatment technology can reduce the risk and pain caused by multiple drug administration or surgery. In order to achieve accurate diagnosis and efficient treatment, the designed diagnostic agents often need to have various functions such as lesion targeting, multimodal imaging and treatment. Rare earth-doped upconversion nanomaterials can convert near-infrared light into visible light, so they have important prospects in the development of non-invasive diagnostic agents. Due to the characteristics of low toxicity, photob...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K41/00A61K9/51A61K49/18A61K49/08A61K47/04A61K47/22A61P35/00
Inventor 陈学元卢珊涂大涛
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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