Environment-friendly technology for treating threonine fermentation mother liquid

A technology of threonine and technology, which is applied in the field of fermentation waste liquid treatment in the biological fermentation industry, can solve the problems of insufficient utilization of waste water, high daily operating costs, and large one-time investment, so as to avoid waste of water resources and high industrial added value , good effect

Active Publication Date: 2015-08-19
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no mature complete set of technology applied to production practice at home and abroad.
The main problem is that the one-time investment is too large, or the daily operation cost is too high, most manufacturers cannot bear it, and have to maintain the status quo of excessive emissions for a long time
[0004] Chinese patent CN 201410559142A discloses an environmentally friendly process for treating threonine fermentation wastewater with biological agents. In this process, probiotics are prepared from bacterial protein, and the wastewater is discharged after being treated with microbial agents; the above process does not make full use of wastewater, resulting in waste , and did not make full use of other sugars, proteins and fiber components other than cell protein, and did not bring profits to the enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] An environmentally friendly process for processing threonine fermented yeast liquid, which comprises the following steps:

[0021] 1) Preparation of threonine crystals and mother liquor: the threonine fermentation broth is passed through a filter with a pore size of 1mm, and the filtrate A and solid fiber matter are collected; the filtrate A is filtered through a microfiltration membrane, and the retentate and filtrate B are collected respectively; then the filtrate B continues to perform ultrafiltration membrane filtration to collect the concentrate and filtrate C; the filtrate C is concentrated to a quarter of the volume of the original solution through an evaporator, then crystallized, and finally centrifuged to collect threonine crystals and waste liquid; combine the above-mentioned retentate, concentrate liquid and waste liquid to obtain mother liquor; wherein, the microfiltration membrane is an inorganic ceramic membrane with a molecular weight cut-off of 3000 Da a...

Embodiment 2

[0026] The present invention uses the granular protein prepared in Example 1 with a purity of more than 90% to replace the yeast powder in the commercially available standard YPD medium, and the rest of the ingredients remain unchanged. Saccharomyces cerevisiae and Pichia pastoris are cultivated under the same conditions. The growth status (OD600) of the product was used to evaluate the cultivation effect of the product. See Table 1 for details:

[0027] Table 1

[0028] group Saccharomyces cerevisiae cultured for 20 hours Pichia pastoris cultured for 20 hours Example 1 2.35 1.64 commercial yeast powder 1.99 1.51

[0029] Conclusion: Determination of OD by nephelometric method 600 Characterizing the growth of the cells shows that this product can replace the commercially available yeast powder products, the cultivation effect is better, and it can bring huge economic benefits to the enterprise.

Embodiment 3

[0031] Feed breeding test prepared by embodiment 1:

[0032] The feed that embodiment 1 prepares is for the influence of chicken growth and development

[0033] Control group: basal diet: ordinary feed = 8:1;

[0034] Test group: basal diet: Example 1 feed = 8:1;

[0035] A total of 40 healthy chicks were selected and randomly divided into two groups, including a control group and a test group, 20 in each group, and the test time was 40 days.

[0036] Weighed on the 1st day and the 40th day respectively, and calculated the average daily weight gain (g / bird) of the chickens in each group after being fed with the feed, as shown in Table 2 for details.

[0037] Table 2 Comparison table of average daily weight gain of chickens

[0038] group Average weight on the first day (g / bird) Average weight on the 40th day (g / bird) Average weight gain (g / bird) control group 42.1 1419.6 1377.5 test group 41.8 1623.9 1582.1

[0039] Conclusion: as can be ...

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PUM

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Abstract

Belonging to the bio-fermentation industry fermentation waste liquid treatment field, the invention discloses an environment-friendly technology for treating threonine fermentation mother liquid. The technology includes the steps of: 1) preparing threonine crystals and a mother liquid, 2) preparing granule protein, 3) preparing chicken feed, and 4) preparing a fertilizer. The technology provided by the invention prepares the granule protein, feed and fertilizer simultaneously, and makes full use of the fermentation mother liquid components. The whole process generates no waste, is environment-friendly and pollution-free, and truly realizes turning waste into wealth.

Description

technical field [0001] The invention belongs to the field of fermentation waste liquid treatment in the biological fermentation industry, and in particular relates to an environment-friendly process for treating threonine fermented yeast liquid. Background technique [0002] Threonine, referred to as Thr, with a chemical formula of C4H9NO3, is an essential amino acid. It is the second limiting amino acid in pig feed and the third limiting amino acid in poultry feed. With the widespread use of lysine and methionine in compound feed Threonine has gradually become the main limiting factor affecting the growth of livestock and poultry. Threonine is mainly used as a feed additive. Its production steps are concentration, crystallization, and extraction of bacterial protein. However, the processing of bacterial protein and mother liquor produced in the process is difficult and costly, and it is difficult to obtain cost-effective by-products. [0003] At present, there are three ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/22C07C227/40C12N1/18C12N1/16C05G1/00A23K1/18A23K1/14A23K1/10A23K1/00C12R1/865C12R1/84
CPCY02P60/87
Inventor 白红兵赵凤良严纪文宋海波侯新明孙钦波
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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