Antifreeze polypeptide composite antifreeze agent and application
An anti-freezing polypeptide and anti-freezing agent technology, which is applied to the preservation of meat/fish with chemicals, can solve the problems of poor flavor, deteriorated quality, and reduced gel properties, so as to increase added value, prevent deterioration, and replenish The effect of calcium absorption
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Embodiment 1
[0031] The raw materials and formula of a kind of fish ball added with compound antifreeze agent are as follows: fish meat: 1kg, potato starch: 50g, salt: 20g, monosodium glutamate: 4g, ginger juice: 4g, pepper powder: 2g, antifreeze polypeptide: 50g , manganese sulfate monohydrate 2mg, anhydrous calcium chloride 4g, sodium lactate 2g, polydextrose 50g, maltodextrin 65g.
[0032] The production steps are as follows:
[0033] (1) Preparation of antifreeze polypeptide: take food-source pigskin collagen as raw material, soak collagen and distilled water for 3 hours at a ratio of 1:100 to make the collagen fully absorb water, add enzyme and substrate at a ratio of 1:50 Alkaline protease, adjust the pH of the enzymatic hydrolysis environment to pH9.0, heat and hydrolyze the collagen in a water bath shaker at 37°C, and the enzymatic hydrolysis time is 3h. After enzymatic hydrolysis, put it in a boiling water bath for 10 minutes to completely inactivate the protease. After cooling t...
Embodiment 2
[0048] The raw materials and formula of a simulated crab meat added with compound antifreeze agent are as follows: fish meat: 1kg, crab meat 0.2kg, potato starch: 65g, salt: 25g, monosodium glutamate: 2g, ginger juice: 5g, pepper powder: 2g , Antifreeze polypeptide: 40g, manganese sulfate monohydrate 3mg, anhydrous calcium chloride 5g, sodium lactate 5g, polydextrose 80g, maltodextrin 55g.
[0049] The production steps are as follows:
[0050] (1) Preparation of antifreeze polypeptide: take food-source pigskin collagen as raw material, soak collagen and distilled water for 3 hours at a ratio of 1:100 to make the collagen fully absorb water, add enzyme and substrate at a ratio of 1:50 Alkaline protease, adjust the pH of the enzymatic hydrolysis environment to pH9.0, heat and hydrolyze the collagen in a water bath shaker at 37°C, and the enzymatic hydrolysis time is 3h. After enzymatic hydrolysis, put it in a boiling water bath for 10 minutes to completely inactivate the protea...
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