Stem cell exosome patch and preparation method and application thereof

Abstract

The invention discloses a stem cell exosome patch and its preparation method and application and belongs to the field of tissue engineering. The stem cell exosome patch comprises the following steps: stem cell is cultured and stem cell exosome is collected; a patch scaffold is prepared; and the exosome obtained is attached to the patch scaffold to prepare the stem cell exosome patch. the stem cell exosome patch obtained has natural and easily available drug sources; action site accuracy of the exosome patch can be guaranteed; effective acting time of the patch after implantation can be prolonged; and potential safety hazard can be avoided to a great extent. Through the method, the stem cell exosome patch with good biological characteristics can be obtained. The invention is a new breakthrough of establishing a biological patch in the field of tissue engineering. Meanwhile, the product also provides a feasible scheme for clinical treatment of diseases. The principle of the invention is scientific and reliable, and the technology is simple and flexible.

Description

technical field [0001] The invention belongs to the field of tissue engineering, in particular to a stem cell-derived exosome patch, a preparation method and application thereof. Background technique [0002] Stem cells are highly differentiated functional cells with proliferation and differentiation potential, self-renewal and replication ability in organisms, and have always been a popular research material in the field of regenerative medicine cell therapy. With the deepening of cell therapy research, stem cell patches prepared by various methods have shown a certain degree of therapeutic effect on the treatment of myocardial infarction, skin trauma, femoral head necrosis, diabetes and other tissue or organ damage, dysfunction and other diseases. Animal or clinical experimental studies have also proved that stem cells can promote the regeneration and repair of necrotic tissue and improve the functions of related tissues and organs. [0003] In traditional cell therapy, s...

AI Technical Summary

Problems solved by technology

[0004] Although the treatment by stem cell patch transplantation can improve the repair of damaged tissues and organs to a certain extent, there are still some defects in the treatment method of stem cell transplantation in vivo: ① shortage of quantity sources: from the perspective of clinical application, except hematopoietic In addition to stem cells and mesenchymal stem cells, many types of stem cells with high clinical application value have limited their clinical application due to extraction difficulties or ethical issues, while stem cells from allogeneic sources are prone to immune rejection after transplantation, such as skin stem cells, limbal stem cells, heart stem cells, and stem cells. Stem cells, embryonic stem cells, etc.; ② It is difficult to maintain the biological activity of stem cells in vitro: Among the various cell sheet construction technologies at present, the research focus is on how to construct a relatively complete cell sheet, and there is no maintenance plan for stem cell activity, including the currently used The most mature and extensive thermosensitive culture method for cell sheet construction

[0007] Since stem cell patches mostly use poorly differentiated stem cells as seed cells, they have high requirements for the living environment, and it is usually difficult to achieve the expected differentiation results after implantation in the body, which affects the therapeutic effect.