Stem cell exosome patch and preparation method and application thereof

An exosome and stem cell technology, applied in the field of tissue engineering, can solve the problems of not involving the maintenance program of stem cell activity, difficult to maintain the biological activity of stem cells, difficult to achieve differentiation results, etc. Protection against adverse effects

Inactive Publication Date: 2015-09-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the treatment by stem cell patch transplantation can improve the repair of damaged tissues and organs to a certain extent, there are still some defects in the treatment method of stem cell transplantation in vivo: ① shortage of quantity sources: from the perspective of clinical application, except hematopoietic In addition to stem cells and mesenchymal stem cells, many types of stem cells with high clinical application value have limited their clinical application due to extraction difficulties or ethical issues, while stem cells from allogeneic sources are prone to immune rejection after transplantation, such as skin stem cells, limbal stem cells, heart stem cells, and stem cells. Stem c...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Cardiac stem cell exosome patch using decellularized pericardium as carrier for treatment of myocardial infarction

[0050] (1) Experimental principle

[0051] Using the natural decellularized pericardium as the patch carrier, exosomes secreted by cardiac stem cells cultured to the third passage in vitro were extracted by filtration centrifugation, and the exosomes were attached to the fibrin gel suspension method. It is hoped that instead of the cardiac stem cell patch, it can greatly achieve the role of the cell patch in improving myocardial infarction.

[0052] (2) Experimental equipment

[0053] Instruments and reagents: pipette gun, centrifuge tube, 100-kDa MWCO hollow fiber membrane (Millipore), PBS phosphate buffer solution (Sigma), 30% sucrose / D 2 O buffer (density 1.210g / cm 3 ), low-temperature ultracentrifuge, 100-kDa MWCO Ultrafree-15 capsules (Millipore), carbonate buffer (0.05mol / L, pH=9.0~9.6), sterile pure water, water bath, sterile PBS sali...

Embodiment 2

[0064]Example 2 The bone marrow mesenchymal stem cell exosome patch using polylactic acid (polylactic acid, PLA) as the patch carrier is used for the treatment of femoral head necrosis

[0065] (1) Experimental principle

[0066] Using synthetic polylactic acid (PLA) as the patch carrier, the bone marrow mesenchymal stem cell exosomes secreted by the bone marrow mesenchymal stem cells cultured in vitro to the third passage were extracted by immunomagnetic bead method. The exosomes were attached to the patch scaffold by the suspension method, in order to replace the bone marrow mesenchymal stem cell patch, but to a great extent achieve the effect of the cell patch on improving the necrosis of the femoral head.

[0067] (2) Experimental equipment

[0068] Instruments and reagents: PBS phosphate buffer (Sigma), serum-free BMSC culture medium without exosomes, incubator, ultracentrifuge, SW28 rotor (Beckman Coulter Instruments, Fullerton, CA), 0.32M sucrose, SW60rotor (Beckman C...

Embodiment 3

[0079] Example 3 Skin stem cell exosome patch using alginate (Alginates) patch as carrier for skin wound treatment

[0080] (1) Experimental principle

[0081] Using natural alginates (Alginates) as the patch carrier, the skin stem cell exosomes secreted by the skin stem cells cultured in vitro to the third generation were extracted by rotary ultrafiltration, and the exosomes were extracted by the nano-peptide gel suspension method. Attached to the patch bracket, it is hoped that it can be prepared to replace the skin stem cell patch but can greatly achieve the effect of the cell patch on improving skin trauma.

[0082] (2) Experimental equipment

[0083] Instruments and reagents: pipette gun, cryogenic centrifuge, centrifuge tube, 0.22 μm filter membrane (Millex-GP, Millipore), sterile PBS phosphate buffer (Sigma), 100nm ultrafiltration centrifuge tube (Amicon Ultra-4, Millipore) , 0.22μm ultrafiltration centrifuge tube (Millipore), D-gluconate hemicalcium salt (1.08% (w / v)...

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PUM

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Abstract

The invention discloses a stem cell exosome patch and its preparation method and application and belongs to the field of tissue engineering. The stem cell exosome patch comprises the following steps: stem cell is cultured and stem cell exosome is collected; a patch scaffold is prepared; and the exosome obtained is attached to the patch scaffold to prepare the stem cell exosome patch. the stem cell exosome patch obtained has natural and easily available drug sources; action site accuracy of the exosome patch can be guaranteed; effective acting time of the patch after implantation can be prolonged; and potential safety hazard can be avoided to a great extent. Through the method, the stem cell exosome patch with good biological characteristics can be obtained. The invention is a new breakthrough of establishing a biological patch in the field of tissue engineering. Meanwhile, the product also provides a feasible scheme for clinical treatment of diseases. The principle of the invention is scientific and reliable, and the technology is simple and flexible.

Description

technical field [0001] The invention belongs to the field of tissue engineering, in particular to a stem cell-derived exosome patch, a preparation method and application thereof. Background technique [0002] Stem cells are highly differentiated functional cells with proliferation and differentiation potential, self-renewal and replication ability in organisms, and have always been a popular research material in the field of regenerative medicine cell therapy. With the deepening of cell therapy research, stem cell patches prepared by various methods have shown a certain degree of therapeutic effect on the treatment of myocardial infarction, skin trauma, femoral head necrosis, diabetes and other tissue or organ damage, dysfunction and other diseases. Animal or clinical experimental studies have also proved that stem cells can promote the regeneration and repair of necrotic tissue and improve the functions of related tissues and organs. [0003] In traditional cell therapy, s...

Claims

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Application Information

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IPC IPC(8): C12N5/0735C12N5/0775C12N5/0789C12N5/0797C12N11/00
Inventor 武征林熙王颖薇严惠泽周清张建华张中夏秦子夕樊泽培路程
Owner JINAN UNIVERSITY
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