Thermal coagulation polysaccharide and fermentation bacterial strain and application thereof

The technology of polysaccharide and strain is applied to thermosetting polysaccharide, its fermentation strain and application field, which can solve the problem of high production cost and achieve the effects of pure white color, simple extraction process and good water absorption performance.

Active Publication Date: 2015-09-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with chemically synthesized plastics, the production cost is relatively high, so many researchers have been working on improving the production intensity of the fermentation method and adopting new extraction techniques in order to reduce production costs and improve market competition.

Method used

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  • Thermal coagulation polysaccharide and fermentation bacterial strain and application thereof
  • Thermal coagulation polysaccharide and fermentation bacterial strain and application thereof
  • Thermal coagulation polysaccharide and fermentation bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 strain screening

[0046] Six soil samples were taken from Jiangnan University in the Wuxi section of the Beijing-Hangzhou Grand Canal. The sample was cultured in screening medium at 30°C and 180rpm for 20 hours, then diluted, spread on a screening plate, cultured at 30°C for 72 hours, and single colonies were selected and cultured by streaking. The strains were inoculated in a Erlenmeyer flask containing 30 ml of screening medium, and cultured at 30° C. and 180 rpm for 80 hours. One of the bottles of fermented liquid showed thermocoagulation properties during the boiling process, and the strain was selected for further research on its fermentation conditions and fermentation products.

[0047] Screening medium: 50 g / L glucose, 1.5 g / L yeast powder, 0.3 g / L peptone, 0.2 g / L beef extract, 1-2 g / L (NH 4 ) 2 SO 4 , 1g / L KH 2 PO 4 , 2g / L CaCO 3 , 0.5g / L MgSO 4 ·7H 2 O, pH 6.5-7.5.

Embodiment 2

[0048] Example 2 Fermentation culture produces PGHX

[0049] (1) Slant culture: Streak inoculate the strain on slant medium (20g / L glycerol, 5 g / L yeast powder, 2 g / L peptone, 2g / L beef extract, 20 g / L agar, pH 6.5-7.5 ), 28-32°C, incubate for 40-60 hours, and set aside.

[0050] (2) Seed culture: Inoculate the slanted seeds in step (1) into the seed medium (20g / L glycerol, 1-5 g / L yeast powder, 2 g / L peptone, 2 g / L beef extract, 1-2 g / L (NH 4 ) 2 HPO 4 , 1 g / L KH 2 PO 4 , 10-20 ml / L corn steep liquor, pH 6.5-7.5), 40-100ml of liquid in a 250ml Erlenmeyer bottle, cultured at 28-32°C for 20-30 hours.

[0051] (3) Fermentation culture: Put the cultured seeds into the fermentation medium (50 g / L glycerol, 1-2 g / L yeast powder, 1-2 g / L (NH 4 ) 2 HPO 4 , 2 ml / L corn steep liquor, 0.5g / L MgSO 4 ·7H 2 O, 0.2 g / L FeSO 4 ·7H 2 O, 0.02 g / L MnSO 4 ·H 2 O, 5g-10g / L NaHPO 4 12H 2 O, 5g-10g / L NaH 2 PO 4 2H 2 O, pH 5.3-7.3), culture temperature 28-32℃, aerobic culture for...

Embodiment 3

[0058] Embodiment 3 product structure analysis

[0059] (1) Monosaccharide composition

[0060] Take an appropriate amount of the sample prepared in Example 2 (extraction method 1), add 2 mol / L trifluoroacetic acid, hydrolyze at 120 ° C for 2 hours under nitrogen protection, remove trifluoroacetic acid, add appropriate amount of deionized water, and use ion chromatography ( HPAEC) to determine the monosaccharide composition. Instrument model: Dionex ICS-5000 (USA), column model: CarboPac PA20 (4 mm × 240 mm).

[0061] The results show( Figure 2a and Figure 2b , Figure 2b Medium sample with Figure 2a The retention time of the standard in the same), the monosaccharide composition of this polysaccharide is galactose.

[0062] (2) Infrared Spectrum

[0063] Mix the sample with KBr and grind it in a mortar until it is extremely fine, and press it into thin slices, using a Nexus infrared chromatograph from Thermo Company, USA at 400-4000 cm -1 scan in the range.

[0064...

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Abstract

The invention discloses a bacterial strain producing novel thermal coagulation polysaccharide (PGHX), and application of bacterial strain, and belongs to the technical field of bioengineering. A method for applying the bacterial strain to prepare the novel thermal coagulation polysaccharide (PGHX) through fermentation, structural characteristics of the fermentation product (PGHX), thermal coagulation performance of the fermentation product (PGHX) and the like are involved. The invention specifically relates to an agrobacterium screening method, the bacterial strain is named as Agrobacterium HX1126 through authentication, and the preservation number is CGMCC10943. The method for producing the fermentation product (PGHX) through microorganisms is achieved. Fermentation liquid with the density of 24.9 g/L can be obtained by conducting fermentation cultivation on the microorganisms under the aerobiotic condition for 48 hours to 90 hours. The polysaccharide (PGHX) produced through the bacterial strain has good thermal coagulation performance, thermal gel can be formed after heating is conducted for 10 minutes under the concentration of 30 g/L at the temperature of 100 DEG C, the gel strength ranges from 245 g/cm<2> to 1450 g/cm<2>. Products have the pure white color and luster. Colloid is in a semitransparent shape and has elasticity.

Description

technical field [0001] The invention discloses a strain of Agrobacterium and a method for fermenting and preparing a novel thermocoagulable polysaccharide (PGHX), and discloses the structural characteristics and thermocoagulability of the fermentation product. Specifically relate to this Agrobacterium screening method, this bacterial strain is identified as Agrobacterium ( Agrobacterium sp. HX1126) with a deposit number of CGMCC 10943, and a method for producing PGHX using the microorganism. Background technique [0002] Every year, the global petrochemical industry produces up to tens of millions of tons of plastics, and some of them are used to produce various packaging boxes, bags, and agricultural mulch films with low added value and are easily lost. These products are scattered on the ground or float on the water surface after use. Difficult to recycle. What's more, due to its non-biodegradability, it has caused increasingly serious ecological environment pollution...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C08B37/00C12R1/01
CPCC08B37/00C12N1/20C12P19/04C12N1/205C12R2001/01
Inventor 刘咏梅郁晓君余晓斌
Owner JIANGNAN UNIV
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