A heat-resistant and stress-resistant SOD modified by genetic engineering and its coding gene and application

A technology encoding gene and amino acid is applied in the field of thermostable superoxide dismutase and its encoding gene and in the field of thermostable oxide dismutase, which can solve the problem that denaturants, organic solvents are easily inactivated, and the product transportation and sales cycle is shortened. , limiting product processing technology and other issues, to achieve the effects of good adaptability, good stress resistance, and simple recombinant expression methods
CN104946606BInactive Publication Date: 2018-11-02北京迈恩泰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京迈恩泰生物科技有限公司
Publication Date
2018-11-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses high-temperature-resistant superoxide dismutase as well as an encoding gene and application thereof. The superoxide dismutase comprises protein with an amino acid residue sequence as shown in SEQ ID NO.1. The expression method comprises the following steps: establishing a recombinant expression vector containing a thermal-resistant superoxide dismutase gene, introducing the established recombinant expression vector into a host cell, and inducing so as to express the thermal-resistant superoxide dismutase gene. The expression product disclosed by the invention is outstanding in thermal resistance, good in adverse resistance, good in resistance to acid, alkali, organic solvents, detergent, protein denaturants and inhibitors, easy to purify, good in stability, and side in industrial application prospect.
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Description

technical field

[0001] The present invention relates to an enzyme and its coding gene and application, in particular to a heat-resistant superoxide dismutase and its coding gene and the heat-resistant superoxide dismutase. Background technique

[0002] Superoxide dismutase (SOD for short) is an enzyme containing metal ions discovered by American scientists Mccord and Fridovich1969 from bovine red blood cells. This enzyme can specifically remove superoxide anion free radicals produced in the process of biological oxidation. It is the body's first line of defense against free radicals and plays an extremely important role in maintaining the dynamic balance of free radical production and removal in biological organisms. effect. Based on the different binding metal prosthetic groups, SOD can be divided into four categories: Cu / Zn-SOD, Mn-SOD, Fe-SOD, and Ni-SOD. SOD has anti-inflammatory, anti-radiation, anti-tumor, anti-aging effects, and is used in the clinical treatment of ...

Claims

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