Alloy nano-particle preparation method based on protein reduction method

A technology of alloy nanoparticles and reduction method, which is applied in the direction of nanotechnology, can solve the problems of accelerating reduction and synthesizing alloys or even ternary alloys, and achieves the effects of increasing synthesis rate, good repeatability, and easy operation

Inactive Publication Date: 2015-10-21
TIANJIN UNIV
View PDF4 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of copper, nickel, ferrous, aluminum and other ions that are easily reduced by proteins to form zero-valent nanoclusters to acce...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alloy nano-particle preparation method based on protein reduction method
  • Alloy nano-particle preparation method based on protein reduction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of bovine lactoglobulin reduced copper-silver alloy nanoparticles, the specific operation steps are as follows:

[0031] (1) Preparation of purified bovine lactoglobulin solution: Weigh a certain amount of bovine lactoglobulin powder and dissolve it in ultrapure water, adjust the pH to 11.0, and centrifuge it after one hour to get the supernatant, and remove it by ultracentrifugation or dialysis Impurity ions in the original protein. Finally, the concentration of the purified bovine lactoglobulin solution was determined to be 4 mg mL by a UV spectrophotometer -1 .

[0032] (2) Noble metal nano-silver uses silver nitrate as a precursor to prepare a 10 mM silver nitrate solution.

[0033] (3) A metal salt with a potential lower than that of the silver redox electrode uses copper sulfate as a precursor to prepare a 20 mM copper sulfate solution.

[0034] (4) Preparation of alloy nano-copper-silver bovine lactoglobulin complex: 500 μL bovine...

Embodiment 2

[0036] Embodiment 2: the preparation of bovine lactoglobulin reduced copper-silver-gold ternary alloy nanoparticles, the specific operation steps are as follows:

[0037] (1) Prepare purified bovine lactoglobulin solution: as described in step (1) in Example 1, the purified bovine lactoglobulin is determined to be 4 mg mL -1 .

[0038] (2) Noble metal nano-gold and silver use chloroauric acid and silver nitrate as precursors to prepare 10mM silver nitrate solution and 20mM chloroauric acid solution.

[0039] (3) A metal salt with a redox electrode potential lower than that of gold and silver was selected as copper chloride as a precursor to prepare a 20 mM copper sulfate solution.

[0040](4) Preparation of alloy nano-copper-silver-golden bovine lactoglobulin complex: put 500 μL bovine lactoglobulin solution, 180 μL copper sulfate solution, 240 μL silver nitrate solution, 100 μL chloroauric acid solution, and 4 mL deionized water into a small glass in the bottle. Adjust the...

Embodiment 3

[0042] Embodiment 3: the preparation of bovine lactoglobulin amyloid fibrils reduced copper-silver alloy nanoparticles, the specific operation steps are as follows:

[0043] (1) Preparation of bovine lactoglobulin amyloid fibrils: as described in step (1) in Example 1, the preparation concentration is 50 mg mL -1 The purified bovine lactoglobulin solution was adjusted to pH 12 and placed in a water bath at 90° C. for 20 hours to obtain a bovine lactoglobulin amyloid fiber solution.

[0044] (2) Noble metal nano-silver uses silver nitrate as a precursor to prepare a 30 mM silver nitrate solution.

[0045] (3) Copper nitrate is used as a precursor for the metal salt with a lower potential than the redox electrode of silver, and a 20 mM copper sulfate solution is prepared.

[0046] (4) Preparation of alloy nano-copper-silver bovine lactoglobulin amyloid fibril complex: put 50 μL bovine lactoglobulin amyloid fibril solution, 180 μL copper sulfate solution, 90 μL silver nitrate so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to an alloy nano-particle preparation method based on a protein reduction method. Firstly, 3-50mg ML<-1> of purified protein solution is prepared; 10-30 mM of precious metal salt solution formed by one of silver, gold, platinum or palladium or mixing any two of the silver, the gold, the platinum or the palladium is prepared; 20-30 mM of metal salt solution of copper, nickel, ferrous iron or aluminum is prepared; the purified protein solution, the precious metal salt solution and the metal salt solution are mixed to prepare a nano alloy precursor solution, the pH of the nano alloy precursor solution is adjusted to an alkaline range through a sodium hydroxide solution, the precursor solution is evenly mixed and then high-purity nitrogen is introduced for deoxygenization, then the mixture is placed in water bath to be heated, and nano alloy quickly reduced by protein can be obtained. Time for preparing precious metal nano-particles reduced by the protein is shortened from 1-5 h to 5-30 min, nano alloy with properties of target nano-particles can be quickly and effectively synthesized, the synthesis rate is greatly increased, and commercial application is facilitated.

Description

technical field [0001] The invention belongs to the field of preparation of metal nanoparticle materials, and relates to a method for preparing alloy nanoparticle based on a protein reduction method. Background technique [0002] Binary or multi-component alloy nanoparticles have broad application prospects in catalysis, optics, biosensing, drug delivery, etc. due to their excellent physical and chemical properties. Alloys can not only have the unique properties of a single metal, but may also produce synergistic effects. Therefore, various new nano-alloy particle combinations have emerged in an endless stream in recent years. [0003] The synthesis of nanoparticles requires reducing and stabilizing agents. Since the addition of chemical reducing agents may have negative impacts on the environment, which is not in line with the development direction of green chemistry, more and more green synthesis methods have emerged as the times require. This includes the use of various...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B22F9/24B82Y40/00
Inventor 苏荣欣朱宏秀齐崴黄仁亮王梦凡
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap