Trichoderma pleuroticola and application thereof

A technology of Trichoderma pleurotus and Trichoderma, applied in the direction of application, chemicals for biological control, biocides, etc., to achieve the effects of fast growth, strong stress resistance and good application prospects

Active Publication Date: 2015-11-04
CHINA KINGDOM AGRITECH QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Trichoderma pleurotus is used to prevent and control soil-borne diseases such as capsicum wilt, chestnut branch blight, and pomegranate dry rot, and has not been reported yet.

Method used

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  • Trichoderma pleuroticola and application thereof
  • Trichoderma pleuroticola and application thereof
  • Trichoderma pleuroticola and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Trichoderma pleurotus strain QZ-001H

[0028] Trichoderma pleurotus strain QZ-001H of the present invention is to collect seaweed from shallow sea reefs in Huangdao District, Qingdao City, and is separated and obtained by dilution plate method and plate streak method, and the separation method is:

[0029] (1) Separation: Seaweeds (obtained from the surface) were collected from shallow sea reefs in Huangdao District, Qingdao City, and all collected samples were stored in sterile bottles, and the collection location, time and collection person were marked. Now wash the seaweed with sterile water, then crush it in a sterile crucible, weigh 1g of seaweed mud in 100mL of sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min , take 100μL 10 -2 、10 -3 、10 -4 The diluted solution was spread on the Bengal Red medium plate, and each gradient was coated in three parallels. After culturi...

Embodiment 2

[0041] 1. Fermentation process of Trichoderma pleurotus strain QZ-001H

[0042] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0043] A large amount of solid fermentation medium formula (mass percentage): solid material: corn cob 50%, bran 40%, rice husk 10%; inorganic salt solution: potassium dihydrogen phosphate 3.5%, magnesium sulfate 0.04%, ammonium sulfate 4% %, the rest is water. The solid-to-liquid ratio is 1:1.8 (mass ratio).

[0044] Mass solid fermentation process of Trichoderma pleurotus strain QZ-001H:

[0045] ①Cultivation of strain seed liquid: Pick a small amount of spores of Trichoderma pleurotus strain QZ-001H from the inclined surface of the test tube, transfer them to PDB liquid medium, and cultivate them on a shaking table at 28°C for 3 to 5 days. This is the seed liquid, and the bacteria live 0.15-0.30cfu / ml;

[0046] ②Cultivation of solid production strains: Inoculate the seed liquid into a solid medium (500mL Erlenme...

Embodiment 3

[0049] This example provides related experiments on the control effect of the original powder of Trichoderma pleurotus strain QZ-001H on pepper wilt.

[0050] 1) Test drug

[0051] Trichoderma pleurotus strain QZ-001H raw powder (prepared in Example 2); 70% thiophanate-methyl wettable powder (commercially available).

[0052] 2) Test crops and control objects:

[0053] The test crop is hot pepper; the variety is Zhongjiao No. 1;

[0054] Control object: Fusarium wilt.

[0055] 3) Test site conditions, test design and arrangement

[0056] The experimental site is located in the chili field of Qingyutun, Nanquan Town, Jimo City, Shandong Province. The soil is sandy land, and the occurrence of Fusarium wilt is relatively heavy in previous years. The cultivation conditions in the experimental field were uniform.

[0057] In this test, the former powder of Trichoderma pleurotus strain QZ-001H is mixed with water to irrigate the roots; 70% thiophanate-methyl wettable powder is ...

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Abstract

The invention discloses trichoderma pleuroticola and application thereof. A strain is a trichoderma pleuroticola strain QZ-001H, and the preservation number is CGMCC No.11049. The invention further discloses a biological control preparation comprising the trichoderma pleuroticola strain QZ-001H, and a preparing method thereof. Experiments show that the trichoderma pleuroticola strain has the advantages of being high in growth speed, large in sporulation quantity, wide in action spectrum and high in stress resistance, a large number of plant roots can be colonized fast, and therefore the trichoderma pleuroticola has a good application prospect. The biological control preparation prepared from the trichoderma pleuroticola strain can prevent and control multiple soil-borne plant diseases including one or more of pepper blight, Chinese chestnut shoot blight, pomegranate dry rot and the like, and has an application prospect.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a Trichoderma pleurotus capable of efficiently preventing and controlling plant diseases and its use, and a biological control preparation prepared by using the Trichoderma Pleurotus. Background technique [0002] Trichoderma (Trichoderma spp.), belonging to the half-knowledge fungi of the Hyphospora, Polypophyles, and Polypodiaceae, is a class of fungi that are widely distributed in soil, air, litter and various fermented products. It can be isolated from the surface of plant rhizosphere, leaves and seeds, and bulbs. Trichoderma is an antagonistic microorganism that is ubiquitous in nature and has abundant resources. Trichoderma has a fast growth and reproduction speed, can quickly occupy the nutrient space, can secrete and produce antibiotics to inhibit the growth of other pathogenic bacteria, and can also achieve the purpose o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01G13/00C12R1/885
Inventor 栾守业牛赡光牛兆颖刘幸红崔凤云
Owner CHINA KINGDOM AGRITECH QINGDAO
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