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Enzyme response type magnetic nano particles and preparation method and application thereof

A magnetic nanoparticle and enzyme-responsive technology, applied in the field of medicine, to achieve good quality, rich sources, and good use effects

Active Publication Date: 2015-11-11
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, the magnetic Fe 3 o 4 Cu 2-x S is the magnetic targeting material and photosensitizer, gelatin and sodium alginate are the composite capsule materials, the construction of new enzyme-responsive magnetic nanoparticles, and its application in tumor targeting therapy have not yet been published.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In concrete implementation, the present invention is realized by the following steps:

[0015] 1) Synthesis of Fe 3 o 4 Nanoparticles: Weigh FeSO 4 ·7H 2 O3.36g and FeCl 3 ·6H 2 O3.2g, dissolved in 80mL ultrapure water, in N 2 Protect and stir at 80°C for 30 minutes, then add 8 mL of ammonia water, the solution turns black, continue to stir for 30 minutes, weigh 1.6 g of citric acid and dissolve it in 3.2 mL of ultrapure water, add it to the above black solution, heat up to 95°C, and react 90min, magnetic separation to obtain precipitate, precipitate washed 3 times, vacuum drying at 60°C to obtain Fe 3 o 4 Nanoparticles;

[0016] 2) Synthesis of magnetic Fe 3 o 4 Cu 2-x S nanomaterials: Weigh 3.6mmoL sublimated sulfur and dissolve it in 2.4mL oleylamine, add 0.16mmoL Fe 3 o 4 In 8 mL of cyclohexane containing nanoparticles, stir at 70°C for 10 min to form sublimated sulfur and Fe 3 o 4 cyclohexane mixed solution; another 0.4mmoL copper acetylacetonate was...

Embodiment 2

[0019] The present invention can also be realized by the following steps in concrete implementation:

[0020] 1) Synthesis of Fe 3 o 4 Nanoparticles: Weigh FeSO 4 ·7H 2 O4.2g and FeCl 3 ·6H 2O4.0g, dissolved in 100mL ultrapure water, in N 2 Protect and stir at 80°C for 30 minutes, then add 10mL of ammonia water, the solution turns black, continue to stir for 30min, weigh 2g of citric acid and dissolve it in 4mL of ultrapure water, then add it to the above black solution, heat up to 95°C, and react for 90min. The precipitate was obtained by magnetic separation, washed three times with water, and dried under vacuum at 60°C to obtain Fe 3 o 4 Nanoparticles;

[0021] 2) Synthesis of magnetic Fe 3 o 4 Cu 2-x S nanomaterials: Weigh 4mmoL sublimated sulfur and dissolve it in 3mL oleylamine, add 0.2mmoL Fe 3 o 4 In 10mL of cyclohexane containing nanoparticles, stir at 70°C for 10min to form sublimated sulfur and Fe 3 o 4 cyclohexane mixed solution; another 0.5mmoL coppe...

Embodiment 3

[0024] In concrete implementation, the present invention is realized by the following steps:

[0025] 1) Synthesis of Fe 3 o 4 Nanoparticles: Weigh FeSO 4 ·7H 2 O50.4g and FeCl 3 ·6H 2 O4.8g, dissolved in 120mL ultrapure water, in N 2 Protect and stir at 80°C for 30 minutes, then add 10mL of ammonia water, the solution turns black, continue to stir for 30 minutes, weigh 2.4g of citric acid and dissolve it in 4.8mL of ultrapure water, add it to the above black solution, heat up to 95°C, and react 90min, magnetic separation to obtain precipitate, precipitate washed 3 times, vacuum drying at 60°C to obtain Fe 3 o 4 Nanoparticles;

[0026] 2) Synthesis of magnetic Fe 3 o 4 Cu 2-x S nanomaterials: Weigh 4.8mmoL sublimated sulfur and dissolve it in 3.6mL oleylamine, add 0.24mmoL Fe 3 o 4 In 12mL of cyclohexane containing nanoparticles, stir at 70°C for 10min to form sublimated sulfur and Fe 3 o 4 cyclohexane mixed solution; another 0.6mmoL copper acetylacetonate was d...

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Abstract

The invention relates to enzyme response type magnetic nano particles and a preparation method and application thereof. The problems of preparation of the enzyme response type magnetic nano particles and application of a magnetic hyperthermia medium and a drug delivery carrier in the oncotherapy can be effectively solved. The method comprises the steps that as a drug delivery carrier system, Fe3O4@Cu2-xS composite nano material is taken as a magnetic-targeted material and a photosensitizer, gelatin and sodium alginate are composite capsule materials, and the magnetic Fe3O4@Cu2-xS composite nano mateiral is prepared into Fe3O4 nano particles through a chemical coprecipitation method; Cu2-xS is loaded on the surfaces of the Fe3O4 nano particles, and a core-shell type Fe3O4@Cu2-xS composite material is obtained; at last, the gelatin and the sodium alginate serve as the composite capsule materials, and the carrier and anticancer drugs are prepared into the enzyme response type magnetic nano particles through a complex coacervation method. According to the enzyme response type magnetic nano particles and the preparation method and application thereof, the sources of the raw materials are rich, the cost is low, the quality is good, the target is strong, the use effect is good, and the enzyme response type magnetic nano particles and the preparation method and application thereof are an innovation for cancer therapeutic drugs.

Description

technical field [0001] The invention relates to medicine, in particular to an enzyme-responsive magnetic nanoparticle and its preparation method and application. Background technique [0002] Tumor is one of the most harmful diseases to humans in the world today. Due to its complex pathogenesis and large gene mutations, traditional therapeutic drugs have little effect in reducing the incidence and mortality of tumors. Near-infrared light-mediated photothermal therapy proposed in recent years can eliminate tumor tissue at a specific point and has low side effects on normal tissue, which provides a new way for tumor treatment. The latest reported semiconducting copper sulfide (Cu 2-x S) Nanoparticles, with simple preparation process, low cost, outstanding photothermal stability and good biocompatibility, have become a research hotspot in the field of nanomedicine. [0003] Tumor invasion and metastasis are the main biological characteristics of malignant tumors and have beco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/42A61K47/36A61K31/704A61P35/00
Inventor 祝侠丽黄河清张振中张英杰侯琳张慧娟李鸿基
Owner ZHENGZHOU UNIV
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