Base fluorescence image capturing system device and method for high-flux genome sequencing

Abstract

The invention discloses a base fluorescence image capturing system device for high-flux genome sequencing. During the genome sequencing process, a to-be-tested DNA fragment sample is placed into a gene chip of a genome sequencer. The base fluorescence image capturing system device comprises a laser control unit and an image capturing unit, wherein the laser control unit is used for controlling parallel linetype laser beams in the Y direction and output by a laser light source to scan the gene chip in X direction and Y direction, and exciting a fluorochrome group carried by nucleotide in the to-be-tested DNA fragment sample to give out light; the image capturing unit is used for separating light emitted by the fluorochrome group so as to obtain a plurality of kinds of light with different wave lengths, and imaging the light by utilizing a TDI camera module. The invention further provides a method utilizing the base fluorescence image capturing system device to capture images, which comprises the following steps: controlling a scanning galvanometer to carry out linear array scanning, converting the optical information into electric signals by utilizing a photovoltaic conversion method as to generate image information finally, and processing through an image processing algorithm to obtain the sequence of the bases of the DNA fragment. With the adoption of the device and the method provided by the invention, high-flux sequencing of the DNA fragment is satisfied, and meanwhile, the device and the method have the advantages of safety and stability, and high degree of automation.

Description

technical field [0001] The invention belongs to the field of biomedical equipment and automatic control, in particular to a high-throughput gene sequencing base fluorescent image capture system device and method. Background technique [0002] The traditional sequencing technology is based on the Sanger sequencing method with the terminal termination method as the core. This method is time-consuming and laborious, requires the participation of a large number of technical personnel, and consumes a lot of financial resources. The next-generation high-throughput sequencing method (also known as deep sequencing (deepsequencing) or next-generation sequencing technology (nextgenerationsequencing, NGS) has achieved a qualitative leap in technology and is a milestone biotechnology platform that can simultaneously convert the entire The genome is sequenced in a short period of time. The development of high-throughput, fully automated and inexpensive sequencing instruments is currently...

AI Technical Summary

Problems solved by technology

The point-scanning confocal fluorescence detection method uses a confocal optical path to remove interfering light, and the laser light is converged into a point to excite fluorescence, which has high detection sensitivity; however, imaging requires two-dimensional mechanical scanning, which increases the measurement time and instrument cost

The surface imaging fluorescence detection method uses devices such as an area array camera to directly detect the fluorescence image of the entire biochip, which has the advantages of short detection time, simple design, and low cost; however, since the excitation light irradiates the entire measured surface, the excitation power per unit area Smaller, so the detection sensitivity is lower

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