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Gas disinfectant for silkworms and application thereof

A disinfectant and gas technology, which is applied in the field of gas disinfectant for silkworms, can solve the problems of increasing the humidity of silkworm raising environment, bacterial diseases of silkworms, and difficulty in drying completely, so as to prevent the occurrence and prevalence of infectious diseases of silkworms, and prevent the occurrence of infectious diseases of silkworms. Disinfection and sterilization of toxic and side effects, and the effect of improving disinfection efficiency

Active Publication Date: 2015-11-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, when the silkworm body and silkworm seat are sprayed for disinfection, although the disinfection effect is better, the humidity of the silkworm seat will increase during the disinfection process; when bleaching powder is mixed with fresh lime powder and used for powder disinfection, it will also be affected by the water absorption of lime Sexuality causes the humidity of the silkworm seat to increase, and the large silkworm stage generally requires a relatively dry breeding environment. Therefore, after the effective disinfection ingredients are lost, it is easy to cause the reproduction of fungi and bacteria, and then cause fungal and bacterial diseases of silkworms (Lu Xingmeng, 2009) Soaking and disinfecting mulberry leaves has a certain effect on the prevention of silkworm microparticle disease, but it is generally difficult to dry completely. If the silkworm is fed directly with moist mulberry leaves, it will cause too much water in the silkworm body and increase the number of silkworms. The humidity of the environment, coupled with the high temperature in summer and autumn, will undoubtedly induce the occurrence of silkworm bacterial diseases and fungal diseases (Lan Bizhong et al., 2014)
In fumigation, most of the disinfectants used are formaldehyde as the main disinfection component. Although they are effective against fungal diseases, they are more irritating to the skin, eyes, and mucous membranes. Formaldehyde is also a recognized carcinogen. et al., 2011)

Method used

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  • Gas disinfectant for silkworms and application thereof
  • Gas disinfectant for silkworms and application thereof
  • Gas disinfectant for silkworms and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The mensuration of embodiment 1 gas chlorine dioxide concentration

[0032] Using the APKS-100 chlorine dioxide gas generator (hereinafter referred to as the disinfection machine), when in use, add an equal volume of stable chlorine dioxide (A liquid) and citric acid activator (B liquid) to the corresponding A, B liquid filling port, seal the cap after adding, open the air outlet when disinfecting, insert a smart magnetic card to control different disinfection time, and then start disinfection, and measure the concentration of gaseous chlorine dioxide at the same time.

[0033] Adopt GT901 portable chlorine dioxide detector, the measuring range is 0~300mg / m 3 If it exceeds this range, it will be calculated according to the formula provided by Apex Environmental Technology (Foshan) Co., Ltd. Calculate the company as follows:

[0034]

[0035] Where: V 1 is the volume of stable chlorine dioxide A solution added to the generator (mL);

[0036] V 2 is the volume of ...

Embodiment 2

[0042] Embodiment 2 is to the disinfection effect of bacillus thuringiensis spore suspension

[0043] Take 1×10 7 The spore suspension per mL was evenly applied to the back of the mulberry leaves (approximately 2 mL per mulberry leaf), and dried in the shade. The control group was directly fed with 3 mulberry leaves. 3 The fifth-instar silkworms were fed after disinfection in a closed space for different periods of time, and were reared at 20-25°C under natural humidity conditions. After 24 hours, they were fed with normal mulberry leaves. Microscopic examination determined the cause of death. After the rearing, the indicators such as the life rate of larvae, the rate of dead cage cocoons, and the unified life of chrysalis were investigated, and the inactivation rate was calculated according to the following formula. The results are shown in Table 2.

[0044]

[0045] Table 2 Disinfection effect of different concentrations and disinfection time on Bacillus thuringiensis ...

Embodiment 3

[0050] Embodiment 3 is to the disinfection effect of Beauveria bassiana conidia suspension

[0051] Adding poison to silkworm body. Take 1×10 7 50 mL of Beauveria bassiana conidia suspension per mL, immerse the five-instar silkworms in each group into the conidia suspension for 1-3 seconds, take out the silkworm body after it is completely soaked and dry it in the air, and let it dry naturally at 20-25°C. Feed under humidity conditions, in which the control group was directly fed, and the test group was fed with mulberry leaves by adding 10mL, 20mL, and 30mL of liquid medicine respectively, and then placed them in the 1m 3 The closed space was disinfected once at different times, and then reared normally. Dead silkworm bodies were collected during the feeding process, and the cause of death was determined according to the appearance characteristics and microscopic examination. After the breeding, the indicators such as the life rate of larvae, the rate of dead cage cocoons, ...

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PUM

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Abstract

The invention relates to a gas disinfectant for silkworms and an application thereof. The gas disinfectant for silkworms is a chlorine-dioxide-containing gas which is generated by reacting raw materials consisting of chlorine dioxide liquor and citric acid liquor in a volume ratio of 1:1. The gas disinfectant disclosed by the invention has the characteristics of a high diffusion rate, uniform distribution, strong penetrability, no residues after disinfection and the like; the chlorine dioxide liquor and the citric acid liquor are combined together for disinfecting air of a silkworm-breeding environment in silkworm-rearing production; the gas disinfectant is simple and convenient, can quickly generate the chlorine-dioxide-containing gas and can disperse the chlorine-dioxide-containing gas to a space including some narrow gaps which need disinfection, and can simultaneously realize all-around disinfection of mulberry leaves, silkworm bodies, a silkworm rearing bed, environment and air, so that the disinfection efficiency is greatly improved. The gas disinfectant disclosed by the invention can be used to effectively control pathogenic microorganisms in the skin-rearing environment to prevent occurrence and spread of silkworm infectious plant diseases, and has disinfection effect which is efficient, safe, broad-spectrum and free of toxic or side effect.

Description

technical field [0001] The invention relates to the technical field of silkworm pest prevention and control, and more particularly relates to a gas disinfectant for silkworms, a disinfection method and an application thereof. Background technique [0002] In recent years, various infectious silkworm diseases are still endangering sericulture production in varying degrees. In the process of silkworm rearing, infectious silkworm diseases such as viral diseases, fungal diseases, bacterial diseases and particulate diseases are all serious diseases to the sericulture industry. It can also be transmitted by breeders or through the air. Therefore, it is particularly important to do a good job of disinfection. At present, there are five main categories of disinfectants commonly used in sericulture production, namely: chlorine-containing disinfectants, including organic chlorine preparations (such as trichloroisocyanuric acid powder, chloramine T, etc.) and inorganic chlorine prepar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/00A01P1/00A01P3/00
Inventor 刘吉平曹荣昌
Owner SOUTH CHINA AGRI UNIV
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