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Natural sericin encapsulated mesoporous silicon nano-carrier and preparation method and application thereof

A nano-carrier, sericin technology, applied in the field of biomedical composite materials, to achieve the effects of high drug loading, good biocompatibility, and high drug loading

Active Publication Date: 2015-12-16
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of sericin-coated mesoporous silicon to prepare drug delivery systems.

Method used

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  • Natural sericin encapsulated mesoporous silicon nano-carrier and preparation method and application thereof
  • Natural sericin encapsulated mesoporous silicon nano-carrier and preparation method and application thereof
  • Natural sericin encapsulated mesoporous silicon nano-carrier and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Preparation of sericin / mesoporous silicon composite nanoparticles by glutaraldehyde crosslinking method provided by the present invention.

[0053] 1. Selection of silkworm cocoons:

[0054] The present invention preferably selects silkworm cocoons of silkworm silk fibroin-deficient mutant varieties (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the Bombyx mori silk fibroin-deficient mutant variety is preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the deposit number is 185Nd-s ) as the raw material, the main chemical component is sericin.

[0055] 2. Extraction of sericin:

[0056] (1) Weigh 2 g cocoons of silkworm silk fibroin-deficient mutant varieties and cut them into 1 cm 2 left and right fragments;

[0057] (2) Submerge the shredded cocoons in 40mL of 0.02M sodium carbonate and dissolve ...

experiment example 1

[0066] Experimental Example 1: Study the biocompatibility of sericin / mesoporous silicon composite nanoparticles.

[0067] 1. After co-incubating the unloaded sericin / mesoporous silicon composite nanoparticles with various tumor cells for 48 hours, the cell viability was detected, such as Figure 4 Shown: When the composite system is not loaded with any drug, there is no obvious effect on the viability of the cells.

[0068] 2. After co-incubating empty sericin / mesoporous silicon composite nanoparticles with mouse-derived macrophages (Raw264.7cells) for 24 hours, the cytoskeleton and nucleus were stained with phalloidin and DAPI to observe The morphology of the cells, and the expression of macrophage inflammatory genes (TNF-α, IL-1β). Such as Figure 5 Shown in A: when macrophages were co-incubated with sericin / mesoporous silicon composite nanoparticles for 24 hours, the morphology of macrophages was similar to that of cells incubated with blank medium (negative control) for ...

experiment example 2

[0069] Experimental Example 2: Detection of fluorescence properties of sericin / mesoporous silicon composite nanoparticles.

[0070] 1. Use a fluorescence spectrum tester to detect the fluorescence spectrum of sericin / mesoporous silicon composite nanoparticles, such as image 3 Shown in A: Under the excitation of an appropriate excitation wavelength, emission light with a wavelength of about 550nm can be generated.

[0071] 2. After co-incubating sericin / mesoporous silicon composite particles with RAW264.7 macrophages for 24 hours, observe the fluorescence of the material with a fluorescence microscope, as shown in image 3 Shown in B: under the excitation of blue light source, the material can emit green fluorescence.

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Abstract

The invention relates to a natural sericin encapsulated mesoporous silicon nano-carrier and a preparation method and application thereof to the field of tumor treatment. The preparation method comprises the steps of preparing pure sericin solids; preparing mesoporous silicon nano-particles; preparing aminated, formylated or dual-sulfurated mesoporous silicon nano-particles with regular pore passage; preparing drug-loaded aminated, formylated or dual-sulfurated mesoporous silicon nano-particles; preparing a pH / protease or reduction / protease dual-responsiveness sericin / mesoporous silicon composite multifunctional control system. The natural sericin encapsulated mesoporous silicon nano-carrier and the preparation method and the application thereof have the advantages that the operation is simple to perform, the raw materials are simple and convenient to obtain, the cost is low, the universal applicability is high, no special complex equipment is needed and the like. The nano composite system prepared by adopting the method fully utilizes the characteristics of the sericin on the outer layer and the mesoporous silicon nano-particles on the inner layer, has pH / protease or reduction / protease dual responsiveness, has multiple functions of inhibiting tumor cells, monitoring nano-particle positioning and drug release in real time and the like, is high is drug loading capacity, is good in biocompatibility and has a wide application prospect in the field of tumor diagnosis and treatment, especially in the aspect of solving multi-drug resistance of tumors.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a variety of natural sericin-wrapped mesoporous silicon nano drug carriers, a preparation method thereof and an application in the field of tumor treatment. Background technique [0002] With the rapid development of nanoscience and technology, a variety of organic or inorganic materials have been made into nanostructures for use in the field of biomedicine, especially the use of nanomaterials as drug carriers for disease treatment has attracted more and more attention. As a solid material with a honeycomb porous structure, mesoporous silica nanoparticles (MSN) have many excellent structural features, such as a large specific surface area and pore volume, uniform and controllable mesopore size pores , stable physical and chemical properties, simple internal and external surface modification and good biocompatibility. Therefore, a lot of research is devoted to using ...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K47/42A61K45/00A61K49/00A61P35/00
Inventor 王琳王征刘佳李琪琳徐鲁明乞超
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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