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Method for quickly detecting tetracosanoic acid of whole blood erythrocytes

A technique for tetracosanoic acid and red blood cells, which is applied in the field of rapid detection of tetracosanoic acid in whole blood red blood cells, and can solve the problem that specific qualitative and quantitative analysis of various fatty acids cannot be achieved, and accurate qualitative separation and quantitative analysis cannot be achieved. Problems such as poor reproducibility of results, to achieve the effect of powerful quantitative function, short time, and improved specificity

Active Publication Date: 2015-12-16
西安金域医学检验所有限公司
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AI Technical Summary

Problems solved by technology

GC is currently a common method for the detection of red blood cell fatty acids, but the detector of this method cannot achieve accurate qualitative separation and quantitative analysis of similar fatty acids that have not been chromatographically separated, so it cannot perform specific and accurate qualitative and quantitative analysis of various fatty acids
The pretreatment of LC-MS / MS method is relatively complicated, and the reproducibility of analysis results is poor
The GC-MS method can solve the disadvantages of GC and LC-MS / MS because it has the separation function of the gas phase and the specific qualitative and quantitative functions of the mass spectrometer. lower volume
[0006] Chinese patent 201210247480.2 discloses a method for detecting fatty acid content by gas chromatography-mass spectrometry, the method is aimed at the sample of animal tissue, the pretreatment of the sample includes a saponification step, and nitrogen protection is required, the pretreatment of the sample is complicated

Method used

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  • Method for quickly detecting tetracosanoic acid of whole blood erythrocytes
  • Method for quickly detecting tetracosanoic acid of whole blood erythrocytes
  • Method for quickly detecting tetracosanoic acid of whole blood erythrocytes

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The pretreatment of embodiment one whole blood sample

[0029] Mix the anticoagulated whole blood sample, centrifuge, discard the upper layer of plasma, white blood cells, platelets, etc.; add an equal volume of normal saline to the remaining red blood cells, mix and centrifuge, discard the upper layer of normal saline; then add the remaining red blood cells Add an equal volume of normal saline and mix well to obtain the separated red blood cell sample.

[0030] Take 50 μL red blood cell sample, put it in a glass centrifuge tube with screw cap, add heptadecanoic acid as an internal standard solution, add 1 mL of 3mol / L hydrochloric acid-methanol solution, mix well, put it in an oven, react at 90°C for 3 hours in the dark, and derivatize After the reaction was completed, cool to room temperature, add 2 mL of n-hexane to extract the methyl esterification reaction product, take the supernatant and dry it with nitrogen, then add n-hexane to the residue as a reconstitution...

Embodiment 2

[0032] Example 2 Detection of whole blood erythrocyte fatty acids

[0033] The sample detection solution is detected by gas chromatography-mass spectrometer.

[0034] The chromatographic conditions include: the chromatographic column is a polar capillary column with a specification of 20m*0.18mm (ID)*0.20μm (film) (purchased from Agilent, model DB-23, PartNumber122-2361), and the carrier gas is 99.999% pure Helium, the carrier gas flow rate is 0.90mL / min, the sampling method is splitless injection, the injection volume is 1 μL, the inlet temperature is 230°C, the post-run temperature is 240°C, the post-run time is 2min, and the purge The flow rate is 4.0mL / min, and the total flow rate is 55mL / min. The temperature programming conditions are shown in Table 1.

[0035]

[0036] Mass spectrometry detection adopts electron bombardment ionization mode, and adopts full scan and selected ion scan methods. Mass spectrometry conditions include: ionization source is electron bomb...

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Abstract

The invention provides a method for quickly detecting tetracosanoic acid of whole blood erythrocytes, and belongs to the technical field of measurement for biotechnique. The method comprises the following steps of (A) pre-treating a whole blood sample; and (B) detecting sample detecting liquid by using a gas chromatography-mass spectrometer. The method has the advantages of small sample amount, easiness in pretreatment, high sensitivity, high specificity, powerful qualitative and quantitative function, short overall detection process, high detection flux and the like.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for rapidly detecting lignoceric acid in whole blood red blood cells. Background technique [0002] In the human body, a small amount of fatty acids exist in free form, and most of them exist in bound form, such as triglycerides, phospholipids, glycolipids, etc. From a nutritional point of view, fatty acids can be divided into essential fatty acids and non-essential fatty acids. Non-essential fatty acids refer to fatty acids that can be synthesized by the body without relying on food supply; The synthesis rate is slow, unable to meet the needs of the body, and must be supplied by food. Essential fatty acids can be divided into omega (Omega)-6 series fatty acids and omega (Omega)-3 series fatty acids. Dietary deficiencies in essential fatty acids can lead to skin lesions, neurological and visual disorders, heart disease and other problems. [0003] Since the...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 燕启江梁耀铭程雅婷赵蓓蓓郭周萍赵强刘菲菲
Owner 西安金域医学检验所有限公司
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