Crocodile small molecule peptide extracted from bone of crocodile and extraction method of crocodile small molecule peptide
A small molecule peptide and crocodile technology, applied in the field of peptide preparation, can solve the problems of inability to extract peptides, restrictions on applicable occasions, waste, etc., and achieve the effect of safe large-scale preparation and use
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Embodiment 1
[0025] The preparation method of the crocodile small molecule peptide comprises the following steps in turn: (1) preparation of starter: use the liquid culture of beneficial bacterial strains to prepare starter, weigh 10 parts by weight of Bacillus subtilis, 10 parts by weight of Helicobacter bifidus, rhamnose 40 parts by weight of Lactobacillus, 15 parts by weight of wort, 20 parts by weight of photosynthetic bacteria, and 5 parts by weight of Bacillus licheniformis were mixed together, and placed in a Erlenmeyer flask filled with culture medium for compound culture. It is a nutrient agar medium, and the temperature for culturing in the Erlenmeyer flask is 37° C., and cultivated for 20 hours at 200 rpm / min, so that the concentration of bacteria or spores reaches 5×10 13 (CFU / L) to prepare starter; (2) Pre-treatment of crocodile bones: fresh crocodile bones are broken, cleaned, and in a cooking tank, adopting a temperature of 150°C and a pressure of 0.17MPa for high-pressure co...
Embodiment 2
[0028] The preparation method of the crocodile small molecule peptide comprises the following steps in turn: (1) preparation of starter: use the liquid culture of beneficial bacterial strains to prepare starter, weigh 10 parts by weight of Bacillus subtilis, 10 parts by weight of Helicobacter bifidus, rhamnose 40 parts by weight of Lactobacillus, 15 parts by weight of wort, 20 parts by weight of photosynthetic bacteria, and 5 parts by weight of Bacillus licheniformis were mixed together, and placed in a Erlenmeyer flask filled with culture medium for compound culture. It is a nutrient agar medium, and the temperature for culturing in the Erlenmeyer flask is 37° C., and cultivated for 19 hours at 220 rpm / min, so that the concentration of bacteria or spores reaches 5×10 13 Individuals / liter (CFU / L) to obtain starter; (2) pretreatment of crocodile bones: fresh crocodile bones are broken, cleaned, and in a cooking tank, adopting a temperature of 140°C and a pressure of 0.17MPa for ...
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