Crocodile small molecule peptide extracted from bone of crocodile and extraction method of crocodile small molecule peptide

Abstract

The invention relates to a crocodile small molecule peptide extracted from bone of crocodile and an extraction method of the crocodile small molecule peptide. The extraction method comprises the following steps in sequence: (1) preparing a fermenting agent; (2) pre-treating bone of crocodile; (3) performing primary microorganism fermentation; (4) performing primary crocodile protein electrolysis; (5) preparing alcohol activated pancreas pulp; (6) performing enzymolysis; (7) post-treating, wherein the percentage of the crocodile small molecule peptide of which the molecular weight is 0-480 Dalton is not less than 87.5%. The crocodile small molecule peptide is applicable to diagnosis peptide, peptide vaccine, antibacterial active peptide, cell factor simulation peptide, anti-virus peptide or anti-tumor peptide, and peptide healthcare products.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to a small crocodile peptide extracted from crocodile bones and an extraction method thereof. Background technique [0002] Amino acid molecules are small and proteins are large. Two or more amino acids are dehydrated and condensed to form several peptide bonds to form a peptide, and multiple peptides are folded in multiple stages to form a protein molecule. Dipeptide, referred to as dipeptide, is a protein fragment composed of two amino acids. Hundreds of peptides have been found in organisms, which are essential participants for the body to complete various complex physiological activities. All cells can synthesize polypeptide substances, and their functional activities are also regulated by polypeptides. It involves the fields of hormones, nerves, cell growth and reproduction, and its importance lies in the regulation of various system organs and cells in the bo...

AI Technical Summary

Problems solved by technology

Then there are many methods for preparing small molecular peptides in the prior art, for example, CN101194733A, CN101532044A, CN102090680A, and CN1280834A all disclose preparation methods about polypeptides, but the method is single and the extraction performance is not high. Another example, CN101120724A discloses a A kind of microbial fermentation is used to make the polypeptide preparation for feed, which is composed of the following raw materials in weight percentage: Bacillus subtilis 40-55, Lactobacillus plantarum 25-35, Lactobacillus casei 10-35

However, when the prepared polypeptide preparation is used to extract polypeptides from bones, the extraction efficiency is not high, the biological fermentation agent is single, and the application occasions are also limited.

CN102488073A discloses a method for extracting sea cucumber peptides, using enzymatic hydrolysis method, adding sea cucumber special enzyme, enzymatic hydrolysis for 6 hours, stirring for 2 minutes every hour during the enzymatic hydrolysis process; adding activated carbon for deodorization, and then adding sodium hydroxide to adjust pH , during the concentration process, the Baume degree is determined to be controlled at 10. This method cannot extract more polypeptides, and the Baume degree is low, resulting in a certain degree of waste of resources.

CN1931020A discloses a low-salt-content polypeptide production method, and its technological process includes: cleaning and crushing fresh cattle and sheep bones, high-pressure cooking, enzymatic hydrolysis with protease, adding acid and alkali to adjust pH value, filtering and concentrating, spraying and drying, Finished product packaging, a wide range of raw material sources, the peptide composition of the finished product can reach 90%, which improves the degree of hydrolysis, and can be used to prepare a variety of health foods. It is suitable for enzymatic extraction of peptides from cattle and sheep animal bones, but for valuable bone raw Can not improve the peptide composition as much as possible, resulting in unnecessary waste

[0004] In summary, the peptide extraction method in the prior art is single, the extraction efficiency is not high, resulting in waste, and the applicable occasions are limited. The prepared peptides, especially small molecular active peptides, are difficult to be used in peptide drugs and peptide health products. Growing Nutraceutical and Pharmaceutical Markets Are Not Significant