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A drug-loading system of catechol-modified hyaluronic acid and its preparation method

A technology of hyaluronic acid and catechol is applied in the directions of pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, which can solve problems such as poor stability, achieve less side effects, and improve bioavailability. the effect of prolonging the retention time

Active Publication Date: 2019-01-11
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its stability is slightly poor, sensitive to strong acid, strong alkali, heat, free radicals and hyaluronidase, and prone to degradation, which limits its application as a drug carrier

Method used

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  • A drug-loading system of catechol-modified hyaluronic acid and its preparation method
  • A drug-loading system of catechol-modified hyaluronic acid and its preparation method
  • A drug-loading system of catechol-modified hyaluronic acid and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Dissolve catechol-modified hyaluronic acid at a concentration of 1.5w / v% in a mixed solvent of dimethylformamide (DMF) / water, add doxorubicin to make the concentration 1.0w / v%, Stir at room temperature until completely dissolved, and let stand to obtain the spinning solution. Transfer the resulting spinning solution to the syringe, connect the spinning device, and adjust the parameters: the applied voltage during spinning is 22KV, the receiving distance is 15cm, and the injection speed is 0.5mL / h; the environmental parameters are set to temperature: 24°C , relative humidity: 45%, after a certain period of time, get nanofiber film, cut into 1×1cm 2 Size, ready to use after drying.

[0054] Cervical cancer cells (HeLa) were cultured on two cell slides, and the two cell slides were placed in a petri dish, and high-sugar DMEM culture solution (10% bovine serum albumin, 1% double antibody) was added, and the The nanofiber membrane obtained above was placed on a slide, cult...

Embodiment 2

[0056] Catechol-modified hyaluronic acid is dissolved in a mixed solvent of dimethylformamide (DMF) / water with a concentration of 1.5w / v%, and doxorubicin is added to make the concentration 1.0w / v%, and the temperature is kept at room temperature. Stir until completely dissolved, and let stand to obtain the spinning solution. Transfer the resulting spinning solution to the syringe, connect the spinning device, and adjust the parameters: the applied voltage during spinning is 22KV, the receiving distance is 15cm, and the injection speed is 0.5mL / h; the environmental parameters are set to temperature: 24°C , relative humidity: 45%, after a certain period of time, get nanofiber film, cut into 1×1cm 2 , after drying for later use.

[0057] Take the HeLa cell suspension in two centrifuge tubes, place the above-mentioned fiber membrane loaded with doxorubicin and the above-mentioned fiber membrane without doxorubicin in the two tubes respectively, take it out after 20 min, add high...

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Abstract

The invention discloses a catechol modified hyaluronic acid drug carrier system and a preparation method thereof. The catechol modified hyaluronic acid drug carrier system is used for in-situ therapy of tumors. In the method, a hyaluronic acid is modified by catechol. The method comprises the following steps of preparing the hyaluronic acid modified by the catechol; preparing a catechol modified hyaluronic acid spinning solution containing an anti-cancer drug; and preparing electrostatic spinning. On the premise that excellent characteristics of the hyaluronic acid are kept, the stability, cell adhesion and other properties of the prepared catechol modified hyaluronic acid are improved. The catechol modified hyaluronic acid has relatively high cell adhesion, so that catechol modified hyaluronic acid nanofibers loaded with adriamycin have good treatment effects on tumor cells.

Description

technical field [0001] The invention belongs to the field of material preparation and drug controlled release, and in particular relates to a catechol-modified hyaluronic acid drug-carrying system and a preparation method thereof, which are suitable for in-situ treatment of tumors. technical background [0002] Electrospinning technology refers to the process of forming fibers from a polymer solution or melt under the action of a high-voltage electrostatic field. It is one of the important methods for preparing one-dimensional nanostructure materials, and it is the only technology that can prepare continuous nanofibers. Electrospinning equipment is simple, easy to design and build according to specific needs, and the output is much larger than other methods of preparing nanomaterials. Nanofibers prepared by electrospinning technology and non-woven fabrics or ordered fibers assembled from nanofibers have large specific surface area and porosity, making them have good applica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K9/70A61K31/704A61P35/00D01D5/00D04H1/728
CPCA61K9/7007A61K31/704A61K47/36D01D5/0007D04H1/728
Inventor 温永强雷霞王文谦杨志林庞铎张学记
Owner UNIV OF SCI & TECH BEIJING
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