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Preparation method and antitumor effect of ginsenoside F1

A technology of ginsenosides and ginseng flowers, which is applied in the field of medicine, can solve the problems of unprepared and purified ginsenosides and low purity, and achieve the effects of no toxic side effects, drug dependence, high purity, and high yield

Active Publication Date: 2016-05-25
北京中云信合科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The above about ginsenoside F 1 The patented technology of separation and preparation, except for the biotransformation method that can obtain monomeric compounds, the rest of the separation and preparation methods are all based on ginsenoside F 1 It is mainly a mixture of ginsenoside F with low purity, and there is no information about the preparation of purified ginsenoside F from natural plants 1 Monomer method reports

Method used

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  • Preparation method and antitumor effect of ginsenoside F1
  • Preparation method and antitumor effect of ginsenoside F1
  • Preparation method and antitumor effect of ginsenoside F1

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Experimental program
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Effect test

Embodiment 1

[0046] The object of the present invention is to provide a kind of active ingredient that prepares a kind of anti-tumor effect-ginsenoside F from ginseng flower methanol extract 1 extraction and purification method. Specific steps are as follows:

[0047] (1) Preparation of Panax ginseng saponins

[0048] Take 4.02kg of freshly dried ginseng flowers, crush them, add 16L of methanol solution, heat and reflux for extraction, 2 hours each time, extract 3 times in total, combine the extracts, recover the solvent under reduced pressure to dryness, and obtain 1130g of methanol extract. Add 500 mL of water to the methanol extract, disperse and dissolve, extract with cyclohexane and ethyl acetate in sequence until the color of the solution becomes significantly lighter, recover and concentrate the ethyl acetate extract under reduced pressure to obtain 355 g of the ethyl acetate extraction fraction. Ginsenoside F 1 of ginseng flower saponins ( figure 1 ), the yield is about 8.8%. ...

Embodiment 2

[0060] (1) Purity test

[0061] Ginsenoside F purified by column chromatography was purified by high performance liquid chromatography (HPLC). 1 Detected, its purity is 95.17% ( figure 2 ). Chromatographic conditions: chromatographic column, ZorbaxEclipseXDBC-18 (5μm; 4.6mmi.d. × 250mm); mobile phase, methanol: water volume ratio of 60:40; flow rate, 1.0mL / min; detection wavelength, 203nm; injection volume, 20 μL. Under this chromatographic condition, ginsenoside F 1 The regression equation of the standard curve is y=2.428x-21.466, R=0.9999; the injection volume has a good linear relationship in the range of 0.3μg-20μg.

[0062] (2) Ginsenoside F 1 structural analysis of

[0063] Using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy, the purified product was characterized, and ginsenoside F 1 MS and NMR spectral features. The instruments selected were Orbitrap Elite mass spectrometer (ThermoScientific, Bremen, Germany) and BrukerDRX-500 n...

Embodiment 3

[0075] Determination of Ginsenoside F by MTT (Tetrazolium Salt) Method 1 Killing effect on human tumor cells.

[0076] (1) Experimental design

[0077] Tumor cell lines used: A549 human non-small cell lung cancer cells, MGC80-3 human gastric cancer cells, and MCF-7 human breast cancer cells. MEM medium).

[0078] Test group:

[0079] Ginsenoside F 1 Group: 1, 25, 50, 100, 200μg / L;

[0080] Ginsenoside Rg 3 Group: 1, 25, 50, 100, 200μg / L;

[0081] Blank control group: cell culture medium;

[0082] Solvent control group: cell culture medium and DMSO;

[0083] Positive control group: 5-fluorouracil (10μg / L)

[0084] (2) method

[0085] Take the cells in the logarithmic growth phase, add 0.25% trypsin to digest, centrifuge at 600r / min for 10min, and adjust the cell concentration to 6×10 4 cells / mL, inoculated in a 96-well culture plate (edge ​​wells were filled with sterile PBS), 90 μL per well. After culturing for 24 hours, add the sample ginsenoside F 1 (sample is d...

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Abstract

The invention discloses an extraction and purification method and a medicine use of ginsenoside, and concretely relates to a separation and purification method of an antitumor effective component ginsenoside F1. The preparation method comprises the following steps: extracting a raw material ginseng flower with methanol, sequentially leaching the obtained extract with cyclohexane and ethyl acetate, and carrying out silica gel column chromatography and MCI GEL column chromatography on the above obtained ethyl acetate extract to obtain a ginsenoside F1 monomer. The preparation method has the characteristics of easy operation, high yield, high purity, no introduction of toxic substances and low cost, and the ginsenoside F1 can be widely used in antitumor medicines.

Description

technical field [0001] The present invention relates to the separation and purification of active ingredients of a natural product and its anti-tumor effect, in particular to ginsenoside F 1 The new separation and purification method of the invention and its effects on lung cancer, gastric cancer and breast cancer belong to the field of medicine. Background technique [0002] In recent years, cancer cases around the world have been increasing rapidly, and it is one of the main diseases that pose a serious threat to human health and life. In my country, the top three cancer incidence rates are lung cancer, breast cancer, and gastric cancer, and the top three mortality rates are lung cancer, liver cancer, and gastric cancer, and the incidence of female cancers has increased significantly, especially breast cancer. Therefore, active and effective prevention and treatment of cancer is still a medical problem to be solved urgently. [0003] Ginseng (Panaxginseng C.A.Mey.) is a ...

Claims

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Application Information

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IPC IPC(8): C07J17/00A61P35/00
Inventor 李珂珂弓晓杰陈丽荣徐斐
Owner 北京中云信合科技有限公司
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