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Preparation method of composite microecological preparation for pigs

A compound microecology and strain preparation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of antibiotic health threats, animal drug resistance, production capacity decline, etc. Effects of food intake, enhancement of immunity, and reduction of feces odor

Inactive Publication Date: 2016-06-15
ZHENJIANG RONGBANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the use of antibiotics, it is easy for animals to develop drug resistance, resulting in the continuous increase of the amount of drugs in the feed, the effect is getting worse, and the number of available drugs is also less and less.
The use of antibiotics will reduce the resistance of animal organisms, and even cause a decline in production capacity. At the same time, the residues of antibiotics also pose a health threat to humans.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the preparation of seed culture medium:

[0016] 1. Preparation of Bacillus coagulans

[0017] 1.1 Bacillus coagulans was obtained from CTCC23316 of China Microorganism Culture Collection Center.

[0018] 1.2 Preparation of bacteria solution

[0019] First prepare glucose 80g / L; corn steep liquor dry powder 1.2g / L; (NH4)SO45g / L; MgSO4 7H2O0.4g / L; KH2PO40.4g / L; Take 0.5ml; CaCO340g / L; pH7.2 1000ml seed culture medium to activate the strain, and then expand the culture step by step.

[0020] 1.3 Preparation of fermentation medium

[0021] In a 65L fermentation tank, bagasse is used as raw material in terms of mass percentage: bagasse 10%, ammonium sulfate 1%, magnesium sulfate 0.4%, potassium dihydrogen phosphate 0.3%, calcium carbonate 4%, pH 7.2.

[0022] 1.4 Viable count

[0023] The total number of viable bacteria was calculated by the dilution coating plate method.

[0024] 1.5 Main equipment

[0025] Shanghai Shuxin Instrument Co., Ltd. microcom...

Embodiment 2

[0046] Embodiment 2: prepare probiotics in this way

[0047] 1. Strain equipment

[0048] Prepare Bacillus coagulans 3.1×10 9 cfu / L, Bacillus subtilis 4.5×10 9 cfu / L, bifidobacteria 1.1×10 9 cfu / L.

[0049] 2. Culture medium preparation

[0050] In terms of mass percentage, the raw materials are mixed evenly and then added. Bran 80%, bagasse 10%, corn flour 3%, ammonium sulfate 1%, magnesium sulfate 0.3%, potassium dihydrogen phosphate 0.3%, manganese sulfate 0.4%, calcium carbonate 4%, sodium chloride 1%, adjust pH Sterilize after reaching 7.0±0.5, add distilled water to adjust the water volume to 10%.

[0051] 3. Inoculation and fermentation

[0052] Firstly, Bacillus subtilis and Bacillus coagulans were mixed according to the volume of 1:1, and then inoculated into the medium with a mass percentage of 20% of the medium, aerobic fermentation was carried out at 25°C for 4 days, and then anaerobic fermentation was carried out for 3 days. % mass percent inoculum was ins...

Embodiment 3

[0053]Embodiment 3: prepare probiotics in this way

[0054] 1. Strain equipment

[0055] Prepare Bacillus coagulans 3×10 9 cfu / L, Bacillus subtilis 5×10 9 cfu / L, bifidobacteria 1.5×10 9 cfu / L.

[0056] 2. Culture medium preparation

[0057] In terms of mass percentage, the raw materials are mixed evenly and then added. 70% bran, 20% bagasse, 4% corn flour, 1% ammonium sulfate, 0.4% magnesium sulfate, 0.3% potassium dihydrogen phosphate, 0.3% manganese sulfate, 3% calcium carbonate, 1% sodium chloride, adjust pH Sterilize after reaching 7.0±0.5, add distilled water to adjust the water volume to 10%.

[0058] 3. Inoculation and fermentation

[0059] Firstly, Bacillus subtilis and Bacillus coagulans were mixed according to the volume of 1:1, and then inoculated into the medium with a mass percentage of 25% of the medium, aerobically fermented at 25°C for 5 days, and then anaerobically fermented for 3 days, with 10 % mass percent inoculum was inserted into bifidobacteria, ...

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Abstract

The invention relates to the preparation of a composite micro-ecological preparation for pigs, which is mainly made of Bacillus coagulans, Bacillus subtilis and Bifidobacterium, which can significantly promote the growth of suckling pigs and fattening pigs, and can improve the immunity of the body at the same time. Improve the environment of the barn.

Description

technical field [0001] The invention relates to a microecological preparation belonging to the category of biological fermentation, in particular to a composite microecological preparation for pig feeding. Background technique [0002] In the past few decades, antibiotics have made great contributions to the development of the aquaculture industry, but at the same time they have brought more disadvantages. With the use of antibiotics, it is easy for animals to develop drug resistance, which leads to the continuous increase of the dosage of drugs in the feed, the effect is getting worse, and the available drugs are also less and less. The use of antibiotics will reduce the resistance of animal organisms, and even cause a decline in production capacity. At the same time, the residues of antibiotics also pose a health threat to humans. Therefore, in recent years, finding alternatives to antibiotics has become more and more important in the animal husbandry industry, and microe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30C12R1/07C12R1/125C12R1/01
CPCC12N1/20
Inventor 杨丽娟杨阳其他发明人请求不公开姓名
Owner ZHENJIANG RONGBANG BIOLOGICAL TECH CO LTD
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