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Method for detecting fingerprint spectrum of cyclocarya paliurus

A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine

Active Publication Date: 2016-06-15
NANJING HAIYUAN CHINESE HERBAL PIECES CO LTD
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  • Abstract
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Problems solved by technology

However, there is currently no literature report on the research on the HPLC fingerprint of Cyclocarya paliurus medicinal material. The present invention establishes the high-performance liquid chromatography fingerprint of Cyclocarya paliurus through high performance liquid chromatography, and compares it with the Chinese medicine chromatographic fingerprint similarity evaluation system. It is of great significance to analyze and establish a control fingerprint, so as to provide a basis for distinguishing other varieties and quality control of Cyclocarya paliurus

Method used

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  • Method for detecting fingerprint spectrum of cyclocarya paliurus
  • Method for detecting fingerprint spectrum of cyclocarya paliurus
  • Method for detecting fingerprint spectrum of cyclocarya paliurus

Examples

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Embodiment 1

[0045] Example 1 Establishment and application of Cyclocarya paliurus control fingerprint

[0046] 1. Instruments and reagents

[0047] ShimadzuLC-20AD high performance liquid chromatography system (Shimadzu Japan), including Shimadzu LC-Solusion workstation, online degasser, SIL-20A autosampler, SPD-20A UV-visible light detector, CTO-20A column temperature Box; BS2242S electronic balance (Beijing Sartorius Instrument System Co., Ltd.); KQ5200DA type numerically controlled ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0048] Kaempferol batch number: 131009 (Nanjing Senbega Biotechnology Co., Ltd.), methanol, phosphoric acid, ethyl acetate, n-butanol (analytical grade, Nanjing Weizhicheng Chemical Reagent Co., Ltd.), acetonitrile (chromatographic grade, American Tiandi Company) ), water (Hangzhou Wahaha Company), 10 different batches of Cyclocarya paliurus medicinal materials were purchased from different medicinal material companies and medicinal material mark...

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Abstract

The invention discloses a method for detecting a fingerprint spectrum of cyclocarya paliurus. The method comprises preparation of a test sample solution, preparation of a control product, establishment of a control fingerprint spectrum and calibration of fingerprint spectrum control peaks. According to structural property characteristics of active components, such as terpenoids, flavonoids, steroids, tannins, organic acids, alkaloids and the like, contained in the cyclocarya paliurus, the best mobile phase composition, elution way, detection wavelength, chromatographic column, column temperature and other analysis conditions are screened through a lot of experiments; many experiments verify and show that the method for detecting the fingerprint spectrum of the cyclocarya paliurus can comprehensively, objectively and accurately detect and evaluate the quality of the cyclocarya paliurus, and has important significance on ensuring clinical efficacy.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a fingerprint detection method of Cyclocarya paliurus. Background technique [0002] Cyclocarya paliurus (Batal.) Iljinskaja is a plant of the Juglandaceae Cyclocarya genus. According to the records of "Chinese Materia Medica", it has the effects of dispelling wind, reducing inflammation and pain, killing insects and relieving itching. Folks mostly use its leaves as medicinal parts, which have a long history of use. Modern studies have shown that Cyclocarya paliurus leaves have the functions of promoting body fluid and quenching thirst, lowering blood sugar, lowering blood pressure, and prolonging life. It can be used to treat diabetes, high blood pressure, and neurasthenia. The existing literature reports that the content of quercetin and kaempferol in Cyclocarya paliurus is used as the quality control standard of medicinal materials, but besides quercetin a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 蔡宝昌陈林伟金俊杰秦昆明郑艳萍张彦南陈丹妮王彬黄雨婷范孟雪
Owner NANJING HAIYUAN CHINESE HERBAL PIECES CO LTD
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