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Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem

A technology of compound lactic acid bacteria and micro-ecological system, applied in the field of micro-ecological preparations, can solve the problems of excessive iodine content in milk and poor effect of bovine mastitis bacteria, and achieve the effects of controlling the number of somatic cells, increasing milk production, and reducing production costs

Active Publication Date: 2016-07-13
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Application No. 201310479603.X "A Cow Udder Cleansing Agent for Preventing Bovine Mastitis" discloses that a cow udder cleaning agent for preventing bovine mastitis consists of 0.1% to 0.5% nisin by mass percentage, 0.02% to 0.2% ε-polylysine, 0.1% to 1.5% disodium EDTA, 2% to 10% glycerin, 0.1% to 1% citric acid, 1% to 5% polyvinylpyrrolidone The purpose is to solve the problem that the existing cow udder cleaning agent has poor effect on inhibiting and killing bacteria that cause mastitis and easily makes the iodine content in milk exceed the standard.

Method used

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  • Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem
  • Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem
  • Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem

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preparation example Construction

[0037] The preparation method of compound lactic acid bacteria nipple cleaning liquid of the present invention comprises the steps:

[0038] a. Activation of strains: Lactobacillus plantarum L.plantarumSCI-01 and L.plantarumSCI-02 frozen at -80°C were inoculated in MRS liquid medium and cultured at 37°C for 24 hours, and subcultured twice to obtain activated bacteria kind;

[0039] b. Preparation of fermentation medium: sucrose 50kg, yeast powder 20kg, soybean peptone 10kg, Tween-800.8kg, water 1000L, fully dissolved and then sterilized at 121°C for 15min;

[0040] c. Preparation of seed culture solution: inoculate the above-mentioned activated strains in the fermentation medium with an inoculum of 1% respectively, and carry out mixed fermentation culture at 37 ° C. During the fermentation process, the pH is controlled by adding ammonia water until the acid production stops. Terminate the fermentation when adding ammonia water again, and aseptically fill the fermented liquid ...

Embodiment 1

[0041] Embodiment 1 Quantitative detection of Lactobacillus, Escherichia coli, Streptococcus agalactiae and Staphylococcus aureus in milk samples by fluorescent quantitative PCR technology, real-time monitoring of the above-mentioned microorganisms in the trial process of the composite lactic acid bacteria teat cleaning solution of the present invention Changes in the content of milk. The Fourier online 400 system was used to measure the change of the number of somatic cells in milk during the trial process of the cleaning solution. The plate count method was used to detect the changes in the total number of colonies, lactic acid bacteria, coliforms, staphylococcus, shigella and salmonella in milk before and after the test.

Embodiment 2

[0042] Example 2 Fourier online 400 system was used to measure the changes of somatic cell number, lactose rate, protein rate and total solids in milk during the trial process of the cleaning solution of the present invention. The melanin test method was used to determine the total number of bacteria in milk according to the time of melanin fading. The changes in the daily milk production of the experimental cows before and after the statistical test were calculated.

[0043] research method:

[0044] 1. Extraction of metagenomic DNA from milk samples

[0045] Microbial metagenomic DNA in milk samples was extracted using the QIAampDNAStoolMiniKit kit. For specific methods, refer to the kit instruction manual. The concentration and quality of metagenomic DNA were detected by micro-volume ultraviolet spectrophotometer.

[0046] 2. Specific primer design

[0047] Four sets of specific primers were designed and synthesized for Lactobacillus, Escherichia coli, Staphylococcus au...

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Abstract

The present invention provides a composite lactobacillus nipple cleaning solution for improving a dairy cow nipple micro-ecosystem.The cleaning solution is prepared by a fermentation solution obtained by fermenting and mixing two plant lactobacillus L.plantarum SCI-01 and L.plantarum SCI-02, the living bacterium concentration of lactic acid bacteria is greater than or equal to 5 billion CFU / mL, the plant lactobacillus L.plantarum SCI-01 and L.plantarum SCI-02 are preserved at the General Microorganism Center of China Committee for Culture Collection of Microorganisms on December 24, 205, and the preservation numbers are respectively CGMCC No.11930 and CGMCC No.11931.Pathogenic bacterium breeding can be effectively inhibited, the somatic cell number can be controlled and the dairy cow nipple micro-ecosystem can be improved by regularly cleaning cow nipples with the cleaning solution, accordingly the recessive and clinical mastitis morbidity is effectively reduced, the milk yield is improved, and the quality of milk is improved.

Description

technical field [0001] The invention relates to a compound lactic acid bacteria teat cleaning solution for effectively improving the micro-ecological system of dairy cow teats. The cleaning solution is composed of Lactobacillus plantarum SCI-01 (L. ) is fermented and belongs to the field of probiotics. Background technique [0002] Bovine mastitis (Bovine Mastitis) is a chronic disease in the global dairy farming industry. It has the characteristics of wide occurrence, high incidence, difficult to cure, and easy recurrence. Both recessive and clinical mastitis will have a direct and indirect impact on the dairy industry chain. The direct impacts include: the milk production and milk quality of dairy cows decline, the toxins produced destroy the mammary epithelial tissue and form scar tissue, resulting in a decline in the production performance of dairy cows throughout the lactation period, and even loss of production performance and early elimination. The indirect impacts ...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P15/14A61P31/04A61P31/10A61P31/02
CPCA61K9/0041A61K35/747A61K2236/19A61K2300/00
Inventor 黄卫强姚国强
Owner INNER MONGOLIA SCI PLUS BIOTECH
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