Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation

Abstract

The invention relates to a method for producing a bacillus amyloliquefaciens bacterial agent through solid-state fermentation. Wheat bran, soybean meal and corn flour are used as main raw materials to make a solid-state fermentation culture medium. The made culture medium is wetted with water, standing culture is performed at the temperature of 28-30 DEG C for 7-8 hours, and then the culture medium is mixed with a chlorine dioxide disinfectant for sterilization at room temperature; the culture medium subjected to sterilization is inoculated with a bacillus amyloliquefaciens seed solution by using the chlorine dioxide disinfectant for solid-state fermentation; after the fermentation is completed, and a solid-state fermented mash is subjected to hot-air drying and smashing to obtain the bacillus amyloliquefaciens bacterial agent, wherein the spore number of the fungicide is up to 200-22 billion / g, and the spore rate is up to 97%-99%. The method adopts chlorine dioxide sterilization to replace traditional high-temperature steam sterilization and is short in time and high in efficiency, the production costs of the bacillus amyloliquefaciens bacterial agent are greatly reduced, and environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing bacillus amyloliquefaciens bacterial agent by solid-state fermentation. Background technique [0002] In agricultural production, crop diseases often occur, causing great economic losses to people. To control crop diseases, chemical pesticides are usually used. Chemical pesticides have the advantages of low cost, quick effect, time-saving and labor-saving, etc. However, the large-scale use of chemical pesticides not only causes great pollution to the environment, but also reduces the quality of agricultural products, and even causes toxic and harmful substances such as pesticide residues to exceed the standard. Biopesticides refer to preparations that use living organisms or their metabolites to kill or inhibit agricultural pests, and have the characteristics of safety, high efficiency, and pollution-free. With the enhancement of people's and gove...

AI Technical Summary

Problems solved by technology

[0009] ① High temperature sterilization needs to consume a lot of heat energy

Whether the heat energy is provided by coal combustion or electric energy, it will greatly increase production costs and cause environmental pollution directly or indirectly.

[0010] ②The high temperature sterilization and cooling of the solid medium takes a long time and the production efficiency is low

[0011] ③High temperature sterilization will cause the destruction of nutrients in the solid medium, and will not use the bacteria to grow