Application of sanguisorba total saponin to treatment of inflammatory bowel diseases

A technology of inflammatory bowel disease and Burnet saponins, which is applied in the field of medicine, can solve the problems of loss of normal flora tolerance and reduced immune response resistance of intestinal bacteria.

Inactive Publication Date: 2016-09-07
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diet and other factors can affect susceptible individuals with low immunity, reducing their resistance to intestinal bacterial immune responses, thereby losing tolerance to normal flora, and finally leading to disease

Method used

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  • Application of sanguisorba total saponin to treatment of inflammatory bowel diseases
  • Application of sanguisorba total saponin to treatment of inflammatory bowel diseases
  • Application of sanguisorba total saponin to treatment of inflammatory bowel diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Qualitative Analysis of Saponins Components in Example 1 Burnet Extract

[0017] Step 1: Analysis of the fragmentation mode of Burnet saponins: Saponins will undergo deglycosylation reaction, 162Da means removal of hexose (glucose, Glc-), 132Da means removal of pentose (arabinose, Ara-). Analysis of the fragment information found that in the secondary fragment mass spectrograms of saponins I and II, 453.33, 471.34 and 585.38 had relatively high intensities. Therefore, we selected the characteristic fragment ions m / z 453.33, 471.34, 585.38 and neutral loss m / z 132, 162 as the characteristic information of saponins, and established the characteristic fragment ion diagnostic technology and neutral loss suitable for the rapid identification of Saponins from Burnet eucalyptus. technology.

[0018] Step 2: Through the characteristic ion diagnosis technology and neutral loss technology, 24 saponin components were comprehensively and rapidly identified for the first time. The ...

Embodiment 2

[0022] Example 2 Identification of In vitro Metabolites of Burnet Saponins in Normal and Inflammatory Bowel Disease Model Rats

[0023] Step 1: Preparation of TNBS enema enteritis model: TNBS was dissolved in absolute ethanol solution (1:1) to prepare a solution with a concentration of 100 mg / kg. Rats were anesthetized by intraperitoneal injection of 7% chloral hydrate. Draw up the prepared TNBS solution with a syringe, fully lubricate the end of the catheter with Vaseline, and enema, the end of the catheter is about 5 cm away from the anus of the rat, slowly push the TNBS in the syringe into the colon, and invert the rat for 1 min. The mice in the control group were given the same dose of normal saline.

[0024] Step 2: Preparation of intestinal flora: Rats were sacrificed by bleeding from the femoral artery, the ileocecal valve was taken out, its contents were exposed, and the contents of the intestinal tract were weighed. Add 3mL of sample preservation solution (20% glyce...

Embodiment 3

[0031] Example 3 Study on Metabolism of Burnet Saponins in Normal and Inflammatory Bowel Disease Model Rats

[0032] Step 1: Experimental animals and grouping: 24 healthy male SD rats were divided into normal group and model group. The model group was given TNBS (100 mg / kg) anally for 7 days, and the two groups were given Burnet saponin (50 mg / kg) by intragastric administration.

[0033]Step 2: collection of biological samples: blank plasma samples were collected before administration, and sacrificed by femoral artery bloodletting 1 hour after intragastric administration, and the plasma of the model group and the control group were respectively collected in tubes added with sodium heparin. After intragastric administration, they were put into metabolic cages, and urine and feces were collected before administration and at 0-24h, 24-48h, 48-72h, and 72-96h after administration.

[0034] Step 3: Biological sample processing: Take 200 μl of plasma from rats in the control group ...

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Abstract

The invention relates to application of sanguisorba total saponin to treatment of inflammatory bowel diseases, relates to a treatment effect of sanguisorba total saponin on inflammatory bowel diseases, belongs to the pharmaceutical field and provides application of sanguisorba total saponin to significant reversal of colon injury caused by TNBS. Experiments prove that sanguisorba saponin can obviously reduce the phenomenon that the weight of rats induced by TNBS and suffered from colitis is reduced and also can obviously reduce the degree of pathological changes of the colitis of the rats induced by TNBS; inflammatory cell infiltration is reduced; the mucosal epithelial degeneration is reduced; TNBS causes severe inflammation response in the rats and also causes increase of expression of proinflammatory factors including TNF-alpha, IL-1beta and IL-6 in serum of the rats; sanguisorba saponin can reverse the phenomenon that the inflammatory factors in the serum of the rats are increased; TNBS simultaneously causes increase of expression of MPO and MDA and reduction of expression of SOD in colon oxygen free radical systems of the rats; therefore, sanguisorba saponin can reverse the phenomenon that the colon oxygen free radical systems of the rats are disordered. The invention further provides influence of sanguisorba saponin on inflammatory factors in LPS-excited RAW 264.7 macrophages; results show that sanguisorba saponin can significantly reduce expression of TNF-alpha, IL-6 and IL-1beta, so that the anti-inflammatory effect is achieved; sanguisorba total saponin can be applied to treatment of inflammatory bowel diseases.

Description

technical field [0001] The invention relates to the therapeutic effect of total saponins of Burnet eucalyptus on inflammatory bowel disease, increases the indications of total saponins of Burnet eucalyptus, and promotes the development of total saponins of Burnet eucalyptus, belonging to the field of medicines. Background technique [0002] Burnet is rich in resources and various types in my country, and there are relatively skilled artificial cultivation techniques that can be used for large-scale propagation. Burnet contains various chemical components such as tannins and polyphenols, triterpenes and their glycosides, flavonoids and their glycosides, and has pharmacological effects such as hemostasis, anti-inflammation, anti-tumor, anti-oxidation and anti-allergy [17-20] . Burnet saponins and their aglycone components are mainly triterpenoid saponins, and their structural types mainly include ursane type, oleanane type, dammarane type and lupine type, among which ursane (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/739A61K31/7048A61P1/00
CPCA61K36/739A61K31/7048
Inventor 梁艳王广基肖竞成李昔诺付涵煦邵宇皓李昊丰
Owner CHINA PHARM UNIV
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