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Production method of compound bacterium solid-state fermented maize protein meal

A corn gluten meal, solid-state fermentation technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of high cost of enzymatic hydrolysis, difficult to control the reaction process, low product conversion rate, etc., to improve immune function, remove bitterness, The effect of improving palatability

Inactive Publication Date: 2016-10-12
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic hydrolysis mainly uses the specific biocatalyst produced by cells to hydrolyze corn gluten powder to obtain corn peptides, but the cost of enzymatic hydrolysis is high, the reaction process in the reaction system is difficult to control, and it is easy to cause differences in the molecular composition of products between batches. difference, and after the reaction is over, it is necessary to change the hydrolysis conditions or heat treatment to inactivate the enzyme, thereby reducing the utilization rate of the enzyme, increasing the production cost, and the conversion rate of the product is low

Method used

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  • Production method of compound bacterium solid-state fermented maize protein meal
  • Production method of compound bacterium solid-state fermented maize protein meal
  • Production method of compound bacterium solid-state fermented maize protein meal

Examples

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Effect test

Embodiment 1

[0027] With reference to the preparation method of the composite bacteria solid-state fermented corn gluten powder of the present embodiment, it comprises the following specific steps:

[0028] (1) Preparation of fermentation raw materials

[0029] Choose the corn gluten powder that passes 40 mesh sieves (0.42mm) as fermentation raw material.

[0030] (2) Preparation of compound bacterial fermentation seed liquid

[0031] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 36 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing on the slant surface for 36 hours, and culture it in shake flask for 12 hours, and the number of viable bacteria is 1.0×10 8 Use a sterile straw to draw a suita...

Embodiment 2

[0037] With reference to the preparation method of the composite bacteria solid-state fermented corn gluten powder of the present embodiment, it comprises the following specific steps:

[0038] (1) Preparation of fermentation raw materials

[0039] Choose the corn gluten powder that passes 40 mesh sieves (0.42mm) as fermentation raw material.

[0040] (2) Preparation of compound bacterial fermentation seed liquid

[0041] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 42 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing it on the slant for 42 hours, and culture it in shake flask for 18 hours, and the number of viable bacteria is 5.0×10 8 Use a sterile straw to draw a suitable l...

Embodiment 3

[0047] With reference to the preparation method of the compound bacteria solid-state fermented corn gluten powder of the present embodiment, comprise the following concrete steps:

[0048] (1) Preparation of fermentation raw materials

[0049] Select corn gluten powder that has passed through a 40-mesh sieve (0.42mm) as the fermentation raw material

[0050] (2) Preparation of compound bacterial fermentation seed liquid

[0051] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 48 hours, inoculate it on the MRS slant culture medium, inoculate it into the liquid medium after culturing it on the slant surface for 48 hours, and culture it in shake flask for 24 hours, and the number of viable bacteria is 1.0×10 9 Use a sterile str...

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Abstract

The invention provides a production method of compound bacterium solid-state fermented maize protein meal and belongs to the technical field of biological feed. The production method comprises the following steps: 1, selecting maize protein meal which is sieved with a 40-mesh sieve as a fermentation raw material; 2, preparing compound bacterium fermented seed liquid with the viable count of 1.0*10<8>cfu / ml to 1.0*10<9>cfu / ml; 3, inoculating the compound bacterium fermented seed liquid into the maize protein meal according to the amount of 6 percent, wherein the moisture content is 50 percent and the pH value is natural; uniformly mixing to obtain a fermentation basic material; 4, spreading the fermentation basic material to the bottom of a fermentation tank according to the thickness of 40cm to 50cm and keeping the temprature of the fermentation tank at 30 DEG C to 35 DEG C; carrying out anaerobic fermentation for 72h to 96h to obtain fermented maize protein meal; 5, drying the fermented maize protein meal at the temperature of 50 DEG C to 55 DEG C and cooling to a room temperature to obtain a compound bacterium solid-state fermented maize protein meal finished product. Maize protein macromolecules can be degraded into small peptides, free amino acids and the like and a bitter taste is effectively removed so that the palatability is improved; the compound bacterium solid-state fermented maize protein meal can be used as a high-quality protein feed resource and can be used for remarkably improving immunity functions of animals when being added into feed.

Description

technical field [0001] The invention belongs to the technical field of biological feed, and in particular relates to a method for preparing solid-state fermented corn gluten powder by compound bacteria. Background technique [0002] Corn gluten powder, also known as corn yellow powder, has a golden yellow granular appearance, a special smell of corn fermentation, and is difficult to dissolve in water. The protein content of corn gluten powder is as high as 55%, starch 15%, fat 7%, fiber content is very low, only 2%, but the amino acid composition is extremely unbalanced, and the leucine content is high (15.1%), but the color Amino acid (1.2%) and methionine (1.8%) content is relatively low, and corn gluten meal itself contains bitter taste and poor palatability, resulting in a very low degree of application in animal feed. [0003] At present, the commonly used corn gluten meal processing methods include enzymatic hydrolysis and microbial fermentation. Enzymatic hydrolysis...

Claims

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Application Information

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IPC IPC(8): A23K10/12
Inventor 魏炳栋陈群邱玉朗王欣李林
Owner JILIN ACAD OF AGRI SCI
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