Kanamycin antibody and preparation process thereof

A kanamycin and preparation technology, applied in the field of kanamycin antibody preparation, can solve the problems of poor inhibitory effect on Gram-positive bacteria and achieve strong operability

Inactive Publication Date: 2016-12-21
陕西思尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aminoglycoside antibiotics are good against Gram-negative bacteria but less effective against Gram-positive bacteria

Method used

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  • Kanamycin antibody and preparation process thereof
  • Kanamycin antibody and preparation process thereof
  • Kanamycin antibody and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kanamycin antibody prepared from the following raw materials in parts by weight:

[0027] Kanamycin sulfate 58 parts, bovine serum albumin 5 parts, ovalbumin 3 parts, Freund's complete adjuvant 3 parts, Freund's incomplete adjuvant 3 parts, carbodiimide 3 parts, glutaraldehyde 1 part , 1 part of goat anti-mouse enzyme-labeled secondary antibody, 2 parts of disodium hydrogen phosphate, 8 parts of sodium acetate, 15 parts of sodium dodecylsulfonate, 10 parts of Coomassie brilliant blue R-250, 8 parts of carbamide peroxide, 6 parts of ethanol 5 parts of formaldehyde, 8 parts of sodium borohydride, 1 part of methanol, 1 part of concentrated sulfuric acid, 2 parts of phosphate buffer, 1 part of carbonate buffer CBS for coating, 1 part of washing solution PBST, 5% of blocking solution 1 part of pig serum, 1 part of ELISA test chromogenic solution, 2 parts of ELISA test termination solution.

[0028] The ELISA test chromogenic solution is 0.25 milliliters; the ELISA test te...

Embodiment 2

[0035] A kanamycin antibody prepared from the following raw materials in parts by weight:

[0036] 69 parts of kanamycin sulfate, 15 parts of bovine serum albumin, 4 parts of ovalbumin, 4 parts of Freund's complete adjuvant, 4 parts of Freund's incomplete adjuvant, 4 parts of carbodiimide, 1.5 parts of glutaraldehyde , 2 parts of goat anti-mouse enzyme-labeled secondary antibody, 2.5 parts of disodium hydrogen phosphate, 9 parts of sodium acetate, 17.5 parts of sodium dodecylsulfonate, 12.5 parts of Coomassie brilliant blue R-250, 10 parts of carbamide peroxide, 8.5 parts of ethanol 1 part, 6 parts of formaldehyde, 10.5 parts of sodium borohydride, 2 parts of methanol, 2 parts of concentrated sulfuric acid, 1 part of phosphate buffer, 1 part of carbonate buffer CBS for coating, 1 part of washing solution PBST, and 5% of blocking solution 1 part of serum, 1 part of ELISA test chromogenic solution, 1 part of ELISA test termination solution.

[0037] The ELISA test chromogenic s...

Embodiment 3

[0044] A kanamycin antibody prepared from the following raw materials in parts by weight:

[0045] 80 parts of kanamycin sulfate, 25 parts of bovine serum albumin, 5 parts of ovalbumin, 5 parts of Freund's complete adjuvant, 5 parts of Freund's incomplete adjuvant, 5 parts of carbodiimide, 2 parts of glutaraldehyde , 3 parts of goat anti-mouse enzyme-labeled secondary antibody, 3 parts of disodium hydrogen phosphate, 10 parts of sodium acetate, 20 parts of sodium dodecylsulfonate, 15 parts of Coomassie brilliant blue R-250, 12 parts of carbamide peroxide, 11 parts of ethanol 1 part, 7 parts of formaldehyde, 13 parts of sodium borohydride, 3 parts of methanol, 3 parts of concentrated sulfuric acid, 1 part of phosphate buffer, 1 part of carbonate buffer CBS for coating, 1 part of washing solution PBST, 5% of blocking solution 1 part of pig serum, 1 part of ELISA test chromogenic solution, 3 parts of ELISA test termination solution.

[0046] The ELISA test chromogenic solution i...

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Abstract

The invention discloses a kanamycin antibody and a preparation process thereof. The kanamycin antibody includes, by weight, 58-80 parts of kanamycin sulfate, 5-25 parts of bovine serum albumin, 3-5 parts of egg albumin, 3-5 parts of Freund's complete adjuvant, 3-5 parts of Freund's incomplete adjuvant, 3-5 parts of carbodiimide, 1-2 parts of glutaraldehyde, 1-3 parts of goat-anti-mouse enzyme labeled secondary antibody, 2-3 parts of disodium hydrogen phosphate, 8-10 parts of sodium acetate, 15-20 parts of sodium dodecyl sulfate, 10-15 parts of Coomassie brilliant blue R-250, 8-12 parts of urea peroxide, 6-11 parts of ethanol, 5-7 parts of formaldehyde, 1-3 parts of a phosphate buffer solution, 1-3 parts of a washing solution PBST, and 1-3 parts of a sealing solution 5% pig serum. With the kanamycin sulfate and the bovine serum albumin as main raw materials, the antibody which can be competitively bond to and can be coated by the enzyme labeled antigen is prepared in a conjugation manner. The whole reaction process is simple, high-effective and economical and is excellent in availability.

Description

technical field [0001] The invention relates to the field of kanamycin antibody preparation, in particular to a kanamycin antibody and a preparation process. Background technique [0002] In recent years, with the continuous expansion of the application of antibiotics and other veterinary drug additives in the field of agriculture and animal husbandry, the problems of antibiotic pollution and veterinary drug residues have become increasingly serious. Antibiotics and other veterinary drugs have a long half-life and are difficult to decompose under natural conditions, causing great harm to the environment. If used as animal food additives without control, antibiotics will form certain residues in animal-derived foods. If humans take such foods containing antibiotic residues for a long time, it will pose a serious threat to health. [0003] Bacterial drug resistance refers to the phenomenon that after repeated exposure of bacteria to a certain drug, the sensitivity to the drug...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K1/34
CPCC07K16/12
Inventor 王作弟
Owner 陕西思尔生物科技有限公司
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