Antibacterial carboxymethyl chitosan/nano zinc oxide composite microsphere and preparation method thereof

A technology of carboxymethyl chitosan and nano-zinc oxide, which is applied in the field of biomedical composite materials, can solve the problems of unsolved dispersibility of nZnO in thin films, the influence of nano-zinc oxide composite materials, and the dispersal situation of nZnO is not involved. Good loading and appearance controllability, safe and non-toxic raw materials, and significant bacteriostatic properties

Inactive Publication Date: 2017-02-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This invention innovatively utilizes the traditional Chinese medicine Panax notoginseng and nZnO to effectively improve the antibacterial performance of the dressing, but it does not involve the dispersion of nZnO in the material, and the preparation of more than eight kinds of raw materials is relatively complicated.
[0008] Chinese patent CN101366969 "Use of Zinc Oxide as an Enhancer for Chitosan Biofilms" adopts the solution casting method to directly pour the acetic acid solution of chitosan on the nZnO powder and dry it to form a film. The blend film prepared by this method has antibacterial properties Good, but the dispersibility of nZnO in the film has not been resolved, and the acidic solution has a great influence on the nano-zinc oxide composite material
At present, there is no research on the preparation of nZnO composite microspheres using carboxymethyl chitosan as a carrier at home and abroad.

Method used

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  • Antibacterial carboxymethyl chitosan/nano zinc oxide composite microsphere and preparation method thereof
  • Antibacterial carboxymethyl chitosan/nano zinc oxide composite microsphere and preparation method thereof
  • Antibacterial carboxymethyl chitosan/nano zinc oxide composite microsphere and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The steps of using carboxymethyl chitosan and nano-zinc oxide (particle size ~ 10nm) to prepare composite microspheres in a mass ratio of 100:8 are as follows:

[0038] (1) Weigh 5g of carboxymethyl chitosan powder (SIGMA-ALDRICH, viscosity 200-800mpa·s) into 300mL of deionized water, and stir with a stirrer. After the powder is completely dissolved, a solution of 1.67wt% is obtained ;

[0039](2) Preparation of nZnO: zinc acetate and dimethyl sulfone were dissolved in methanol to prepare a solution of 0.015 mol / L, and 0.001 mol / L methanol solution of potassium hydroxide was slowly dropped into it, and a transparent solution was obtained after the reaction for 12 hours. Add deionized water to precipitate the nanoparticle crystals, centrifuge and wash the precipitate with ethanol, and then dry to obtain powdery nZnO;

[0040] (3) Weigh 0.4 g of nZnO powder (about 10 nm in diameter) obtained in step (2), and add it to the carboxymethyl chitosan obtained in step (1) at a ...

Embodiment 2

[0044] The steps of using carboxymethyl chitosan and nano-zinc oxide (particle size ~ 30nm) to prepare composite microspheres in a mass ratio of 100:8 are as follows:

[0045] (1) Weigh 5g of carboxymethyl chitosan powder (SIGMA-ALDRICH, viscosity 200-800mpa·s) into 300mL of deionized water, and stir with a stirrer. After the powder is completely dissolved, a solution of 1.67wt% is obtained ;

[0046] (2) Weigh 0.4 g of nano-zinc oxide powder (ALADDIN, diameter 30±10 nm), and add it to the carboxymethyl chitosan solution obtained in step (1) at the ratio of nano-zinc oxide / carboxymethyl chitosan=8wt% And use the ultrasonic oscillator for 20min to disperse, and let it stand for later use after the dispersion is uniform;

[0047] (3) the mixed suspension obtained in step (2) is spray-dried with a spray dryer, the inlet temperature is 220 ° C, the air intake is 90%, the feeding rate is 7.5 mL / min, and the obtained composite microsphere powder is stored Reserve in a moisture-pro...

Embodiment 3

[0050] The steps of using carboxymethyl chitosan and nano-zinc oxide (particle size ~ 30nm) to prepare composite microspheres in a mass ratio of 100:6 are as follows:

[0051] A preparation method of carboxymethyl chitosan / nano zinc oxide composite microspheres, comprising the following steps:

[0052] (1) Weigh 5g of carboxymethyl chitosan powder (SIGMA-ALDRICH, viscosity 200-800mpa·s) into 300mL of deionized water, and stir with a stirrer. After the powder is completely dissolved, a solution of 1.67wt% is obtained ;

[0053] (2) Weigh 0.3 g of nano-zinc oxide powder (ALADDIN, diameter 30±10 nm), add it into the carboxymethyl chitosan solution at the ratio of nano-zinc oxide / carboxymethyl chitosan=6wt%, and shake with ultrasonic The instrument is shaken for 20min to disperse, and after the dispersion is uniform, let it stand for standby;

[0054] (3) the mixed suspension obtained in step (2) is spray-dried with a spray dryer, the inlet temperature is 220 ° C, the air intake...

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Abstract

The invention belongs to the technical field of bio-medical composite materials, and discloses an antibacterial carboxymethyl chitosan / nano zinc oxide composite microsphere as well as a preparation method and an application thereof. The preparation method comprises the following steps: adding nZnO to a water solution of carboxymethyl chitosan, conducting ultrasonic oscillation so that the nZnO is uniformly dispersed, and conducting standing by so as to obtain a mixed suspension of the carboxymethyl chitosan and the nZnO; and spray-drying the suspension by virtue of a spray-dryer, so that the carboxymethyl chitosan / nano zinc oxide composite microsphere is obtained. The carboxymethyl chitosan / nano zinc oxide composite microsphere provided by the invention is small in grain size and obvious in antibacterial activity, and an antibacterial rate on staphylococcus aureus and escherichia coli reaches 80% or above; and the carboxymethyl chitosan / nano zinc oxide composite microsphere is low in biotoxicity, and the survival rate of NIH-3T3 cells within 24h is higher than 90% when the concentration of the composite microsphere is less than or equal to 125mg / mL; therefore, the carboxymethyl chitosan / nano zinc oxide composite microsphere can be applied to wound repair materials, food / drug packaging materials and drug carrier materials.

Description

technical field [0001] The invention belongs to the technical field of biomedical composite materials, in particular to an antibacterial carboxymethyl chitosan / nano zinc oxide composite microsphere and a preparation method and application thereof. Background technique [0002] Nano-zinc oxide (nZnO) is a nano-metal oxide with excellent bacteriostatic properties, and the U.S. Food and Drug Administration (FDA) has listed nZnO as one of five safe zinc compounds. It has strong antibacterial ability, no irritation, good thermal stability, abundant sources, low price, and itself is white. Therefore, nZnO has been gradually used as an antibacterial agent in cosmetics, textiles and sanitary ceramics in recent years. [0003] However, the properties of nanomaterials are obviously different from ordinary materials, and their potential harm to human health is not yet clear. Many studies at home and abroad have proved that the smaller the particle size and the larger the concentration...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/18A61L15/28A61L15/46A61L26/00A61K47/36A61K47/02C08L5/08C08K3/22
CPCA61L15/18A61K47/02A61K47/36A61L15/28A61L15/46A61L26/0004A61L26/0023A61L26/0066A61L2300/102A61L2300/404C08K3/22C08K2003/2296C08K2201/011C08L5/08
Inventor 钟全李红周长忍
Owner JINAN UNIVERSITY
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