Plant bacteriostatic composition and application thereof in daily necessities
A plant antibacterial and composition technology, applied in the field of daily necessities, can solve problems such as mutagenesis, skin irritation, teratogenicity, etc., and achieve good anti-infection and good antibacterial effects.
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Embodiment 1-4
[0014] Embodiment 1-4 A kind of plant antibacterial composition
[0015] Table 1 is the consumption of each component in a kind of plant antibacterial composition of embodiment 1-4.
[0016] Table 1
[0017]
[0018] The preparation method of a kind of plant antibacterial composition described in embodiment 1-4 is as follows: take each component, pulverize, sieve with 60 mesh sieves respectively, mix, add volume fraction by liquid material mass ratio 15:1 is 50% ethanol, after soaking for 2 hours, use a high-pressure homogeneous extractor to extract at room temperature under the extraction pressure of 40MPa. After the extract is centrifuged, it is concentrated with a membrane. 40%, 70%, 95% ethanol was eluted multiple times, the eluate was collected, and the membrane was concentrated until the mass ratio of the extract to the plant component was 1:1, then dissolved in butanediol and filtered to obtain the plant inhibitor bacteria composition.
experiment example 1
[0028] Experimental example 1 antibacterial experiment
[0029] The tested bacteria were bacteria such as Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. Resurrect the above bacterial species and culture them under the following conditions respectively. Bacteria use lecithin Tween 80-nutrient agar medium and culture at 30-37°C for 36-48 hours; Aspergillus niger use Bengal red medium and culture at 20-25°C for 120 hours; For Candida albicans, use potato dextrose agar medium and culture at 20-25°C for 24-48 hours. The bacterial concentration was diluted to 1.0×10 9 CFU / mL, the concentration of Candida albicans was diluted to 1.0×10 9 CFU / mL, the Aspergillus niger concentration was diluted to 1.0×10 7 CFU / mL. Pipette 0.1 mL of the above-mentioned bacterial dilution on the plates containing the corresponding solid medium, spread evenly with a spreading rod, put 4 Oxford cups equidistantly in each plate, and add 0.1 mL...
experiment example 2
[0033] Experimental example 2 irritation test
[0034] The plant antibacterial composition of Example 1 and the traditional bacteriostatic agent of Comparative Example 6 were subjected to the chicken chorioallantoic membrane blood vessel test, and the reaction irritation of the blood vessels was observed, which reflected the irritation of the sample to the skin. The experiment was carried out by utilizing the characteristics of complete, obvious and transparent blood vessels of CAM (ie, fertilized mid-stage chorioallantoic membrane of hatched 10-14-day embryonic embryo). The plant antibacterial composition and the traditional bacteriostatic agent of Comparative Example 6 are contacted with 2 CAMs respectively, act for a period of time, observe the degree of CAM vascular damage, and score the irritation, the more serious the vascular damage, the greater the score, the greater the irritation. bigger. Judgment criteria are shown in Table 3, and irritation results are shown in Ta...
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