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Total serum protein detection kit with strong anti-interference capability

A detection kit and total protein detection technology, which is applied in the field of serum total protein detection, can solve the problems of expensive antibody, slow color development, and not widely used, and achieve the effect of good repeatability, simple operation, and good accuracy

Inactive Publication Date: 2017-02-22
济南中安生物技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The BCA method has good sensitivity, accuracy and repeatability, and is less affected by lipid turbidity, hemolysis, and bilirubin, but the color development is slow, so it is not suitable for use with automatic biochemical analyzers; the dye-binding method is easy to use, fast, and has good repeatability , has a good correlation with the biuret method, but is easily affected by lipemia, hemolysis and hyperbilirubinemia; the immunoturbidimetric method has strong specificity, high accuracy, and good repeatability , Hemolytic interference is small, suitable for use with automatic biochemical analyzers, but the antibody is expensive and not widely used in China; and IFCC recommended method, but it is susceptible to the interference of fat turbidity, hemolysis, dextran, and high concentration of calcium and magnesium ions will also affect the test results

Method used

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  • Total serum protein detection kit with strong anti-interference capability
  • Total serum protein detection kit with strong anti-interference capability
  • Total serum protein detection kit with strong anti-interference capability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Reagent R composition:

[0034] Copper sulfate pentahydrate 3g / L

[0035] Potassium iodide 5g / L

[0036] Sodium Potassium Tartrate 18g / L

[0037] Potassium hydroxide 8.0g / L

[0038] Tween-20 0.8%

[0039] Polyoxyethylene lauryl ether 5g / L

[0040] EDTA·Na 2 6.7g / L

[0041] Urea 4.6g / L

[0042] Triton x-100 0.1%

[0043] The usage method of embodiment 1 reagent:

[0044] The serum total protein detection reagent described in this example is used in a fully automatic biochemical analyzer, such as Hitachi 7180 fully automatic analyzer, etc., and is measured by the one-point endpoint method. Set the ratio of sample and reagent R to 6:300, place distilled water, standard and sample in the corresponding position of the sample plate, the operation is as follows image 3 .

[0045] Urinary total protein content (g / L) = (∆A measurement ÷ ∆A standard) × C standard.

Embodiment 2

[0047] Interference test: Take fresh mixed urine, divide it into 2 equal parts, then divide each part into 5 equal parts, according to Figure 4 The concentration requires adding different interfering substances, and then use the reagents obtained in Example 1 to compare and measure the content of total urinary protein with the common and recognized urine total protein reagents in the market. See the test results Figure 4 . Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0048] Depend on Figure 4 It can be seen that the test results of the reagent of Example 1 are dextran≤100g / L, bilirubin≤100mg / L, calcium ion≤4mmol / L, magnesium ion≤5mmol / L, triglyceride≤22.6mmol / L No apparent interference. Consistent with the test results of the control reagent, it shows that the reagent of Example 1 is up to the standard in accuracy, and the anti-interference ability is ob...

Embodiment 3

[0050] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the serum total protein kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are as follows: Figure 5 shown. And obtained the correlation curve of the two reagents (such as figure 1 As shown), the test results show that the correlation coefficient of the two kits is 0.999, indicating that there is a great correlation between the two.

[0051] The calibrators and quality controls used in the test were:

[0052] RANDOX938 calibrator: the content of total serum protein is 63.0g / L;

[0053] RANDOX1005 quality control substance: the target value of serum total protein is 59.3g / L, and the target value range: 47.5-71.1g / L.

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Abstract

The invention discloses a total serum protein detection reagent. By virtue of improvement on the basis of a biuret determination method, potassium hydroxide is used for replacing sodium hydroxide, and interference of dextran on detection result is eliminated; two non-ionic surfactants, namely polyoxyethylene laurel ether and Tween-20, are used, so that solubility of the reagent is enhanced, and process of reaction can be more benefited. The reagent has good accuracy and repeatability as well as good detection range and is beneficial to clinically popularization and application of the reagent.

Description

technical field [0001] The invention relates to the technical field of serum total protein detection, in particular to a serum total protein detection reagent with strong anti-interference ability. Background technique [0002] Serum total protein (TP) is a type of macromolecular compound with the most content in serum except water. It is a general term for various proteins contained in serum. TP contains a variety of proteins with special functions, such as enzymes, immunoglobulins, interferon, growth hormone, etc. Serum total protein can be divided into albumin (albumin) and globulin into two categories. Normal human albumin is 38~48g / L, and globulin is 15~30g / L. Plasma protein has multiple functions such as maintaining normal colloid osmotic pressure and pH in blood vessels, transporting various metabolites, and regulating the physiological effects of the transported substances, and is closely related to the immune function of the body. In patients with chronic adrenal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78G01N21/82
CPCG01N21/31G01N21/78G01N21/82G01N2021/3185
Inventor 罗维晓王进谭柏清王美丽
Owner 济南中安生物技术服务有限公司
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