Liver stem cell injection and preparation method thereof

A technology of hepatic stem cells and injections, applied in the field of biomedicine, can solve problems such as the establishment of safe and reliable human hepatic stem cell lines, and achieve better therapeutic effects, prolong the number of passages, and shorten the growth cycle

Active Publication Date: 2017-03-08
BEIJING YULONG SHENGSHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a safe, reliable, fully functional and immortalized human liver stem cell line has not been established so far.

Method used

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  • Liver stem cell injection and preparation method thereof
  • Liver stem cell injection and preparation method thereof
  • Liver stem cell injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A hepatic stem cell injection for treating diabetes, each ml injection mixture contains 1×10 7 hepatic stem cells and 1×10 3 umbilical cord mesenchymal stem cells; the injection mixture consists of the following mass percentage components: 2% polyethylene glycol, 2% arginine, 3% lysine, 2% methionine, 4% % valine, 0.2% glacial acetic acid, 3% collagen, 2% glycosaminoglycan, 0.3% retinoic acid and 0.8% ethanolamine; the balance is normal saline.

Embodiment 2

[0035] A hepatic stem cell injection for treating diabetes, each ml injection mixture contains 3×10 5 hepatic stem cells and 1×10 4 umbilical cord mesenchymal stem cells; the injection mixture consists of the following mass percentage components: 8% polyethylene glycol, 5% arginine, 7% lysine, 6% methionine, 7% % valine, 0.8% glacial acetic acid, 5% collagen, 5% glycosaminoglycan, 0.3-0.5% retinoic acid and 1.2% ethanolamine; the balance is normal saline.

[0036] Preparation:

[0037] 1) Culture of liver stem cells;

[0038] 2) Culture of umbilical cord mesenchymal stem cells;

[0039] 3) Polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycan, retinoic acid and ethanolamine are dissolved in normal saline to prepare a compound solution, Pre-cool at 4°C for later use;

[0040] 4) Resuspend the hepatic stem cells obtained in step 1) and the umbilical cord mesenchymal stem cells obtained in step 2) in the compound solution...

Embodiment 3

[0042] A hepatic stem cell medium, based on DMEM / F12 medium, comprising the following components at concentrations: 6mg / ml hyaluronic acid, 60ng / ml hepatocyte growth factor, 50ng / ml basic fibroblast growth factor, 10μg / ml ml recombinant human bone morphogenetic protein, 300 μg / ml 2′,3′-dideoxyadenosine-5-triphosphate, 7 mg / ml ferric citrate, 10 mg / ml carboxymethylcellulose sodium and 3 mg / ml L-carnitine .

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Abstract

The invention relates to a liver stem cell injection and a preparation method of the liver stem cell injection. The injection is prepared from liver stem cell, umbilical cord mesenchymal stem cell, polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycan, retinoic acid, and ethanol amine according to a certain component ratio. In the liver stem cell injection, the liver stem cell is matched with the umbilical cord mesenchymal stem cell, thus the treatment effect of diabetes is better; the injection formula can maintain the activity of the liver stem cell for a long time and is free from any toxic and side effects; the injection formula is safe and effective, low in cost, and can be directly applied to the clinical injection of diabetes. The liver stem cell cultivating method and its special culture base can effectively maintain the best state of the liver stem cell, guide the cell proliferation while keep the dryness of the liver stem cell, and reduce the damage rate and death rate of the liver stem cell in the cultivating and harvesting processes, and shorten the growth cycle.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a liver stem cell injection and a preparation method thereof. Background technique [0002] In the early 20th century, some scholars speculated through some experimental phenomena that the mature liver has a strong regenerative capacity, because there is a group of "ancestor" cells that can differentiate into liver cells and bile duct cells. At present, people have formed a broad consensus on the existence of liver stem cells. It is a kind of stem cells with self-renewal ability and multi-directional differentiation potential. It can produce stem cells with the same phenotype and genotype as itself through its own symmetric and asymmetric division. , can also produce cells of different developmental stages and different differentiation directions in liver tissue, such as hepatocytes, bile duct epithelial cells and pancreatic epithelial cells. With the in-depth understanding of its cha...

Claims

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Application Information

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IPC IPC(8): A61K35/407A61K35/28A61K9/10A61P3/10C12N5/0735A61K47/42
Inventor 张晓南吴芳春冯传前邵来
Owner BEIJING YULONG SHENGSHI BIOTECH CO LTD
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