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A kind of fibroin/bacterial cellulose composite hydrogel and its preparation method and application

A bacterial cellulose and composite hydrogel technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problems affecting the biocompatibility of bacterial cellulose and silk protein composite materials, the hidden dangers of the safety performance of biological materials, and the impact of materials It can improve the histological morphology and biochemical properties of cartilage, promote load and stable dispersion, and accelerate the repair of full-thickness articular cartilage defects.

Active Publication Date: 2019-04-16
江苏臻中滤料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composite material uses the carboxyl group on the surface of the modified bacterial cellulose to combine with the silk protein, and then combines the bacterial cellulose and the silk protein through the action of a cross-linking agent to form a composite material. The material contains a chemical cross-linking agent. The presence of bacteria will inevitably affect the biocompatibility of bacterial cellulose and silk protein composite materials, cause hidden dangers to the safety performance of biological materials, and affect the environmental protection and safety performance of materials

Method used

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  • A kind of fibroin/bacterial cellulose composite hydrogel and its preparation method and application
  • A kind of fibroin/bacterial cellulose composite hydrogel and its preparation method and application
  • A kind of fibroin/bacterial cellulose composite hydrogel and its preparation method and application

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Effect test

Embodiment 1

[0031](1) Acetobacter xylinum is used as the strain, and liquid seed medium, Acetobacter xylinum slant medium and fermentation medium are configured, and after high-temperature sterilization at 121° C. for 20 minutes, the seed medium and the activated strain slant are taken, Use an inoculation loop to pick two loops of bacteria into the liquid medium, and the whole process is aseptically operated. After the medium was shaken evenly, it was placed in a temperature-controlled shaker cabinet, and cultured at a temperature of 30° C. and a rotational speed of 160 r / min for 24 hours. Put the cultured seed liquid into the fermentation medium, and cultivate it at a constant temperature of 30°C for 10 days, and form a gelatinous bacterial cellulose film at the interface between the medium and the air, take out the gelatinous bacterial cellulose film, and use After rinsing with deionized water, immerse in 0.5mol / L NaOH aqueous solution, treat at 80°C for 2 hours, and then immerse in dei...

Embodiment 2

[0037] (1) Acetobacter xylinum is used as the strain, and liquid seed medium, Acetobacter xylinum slant medium and fermentation medium are configured, and after high-temperature sterilization at 121° C. for 20 minutes, the seed medium and the activated strain slant are taken, Use an inoculation loop to pick two loops of bacteria into the liquid medium, and the whole process is aseptically operated. After the medium was shaken evenly, it was placed in a temperature-controlled shaker cabinet, and cultured at a temperature of 30° C. and a rotational speed of 160 r / min for 24 hours. Put the cultured seed liquid into the fermentation medium, and cultivate it at a constant temperature of 30°C for 10 days, and form a gelatinous bacterial cellulose film at the interface between the medium and the air, take out the gelatinous bacterial cellulose film, and use After rinsing with deionized water, immerse in 0.5mol / L NaOH aqueous solution, treat at 80°C for 2 hours, and then immerse in de...

Embodiment 3

[0043] (1) Acetobacter xylinum is used as the strain, and liquid seed medium, Acetobacter xylinum slant medium and fermentation medium are configured, and after high-temperature sterilization at 121° C. for 20 minutes, the seed medium and the activated strain slant are taken, Use an inoculation loop to pick two loops of bacteria into the liquid medium, and the whole process is aseptically operated. After the medium was shaken evenly, it was placed in a temperature-controlled shaker cabinet, and cultured at a temperature of 30° C. and a rotational speed of 160 r / min for 24 hours. Put the cultured seed liquid into the fermentation medium, and cultivate it at a constant temperature of 30°C for 10 days, and form a gelatinous bacterial cellulose film at the interface between the medium and the air, take out the gelatinous bacterial cellulose film, and use After rinsing with deionized water, immerse in 0.5mol / L NaOH aqueous solution, treat at 80°C for 2 hours, then immerse in deioni...

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Abstract

The invention provides a fibroin / bacterial cellulose composite hydrogel, and a preparation method and application thereof. According to the invention, bacterial cellulose hydrogel surface-modified by glycidyl trimethyl ammonium chloride, bone morphogenetic protein-2 and fibroin are used as raw materials; electrical gel technology is employed for formation of a network pore structure by the fibroin on the surface and in the internal network pore structure of the surface-modified bacterial cellulose hydrogel so as to compose a dual-network pore structure; and the morphogenetic protein-2 is adhered into the dual-network pore structure so as to form the fibroin / bacterial cellulose composite hydrogel. The preparation method is simple, highly efficient, green and environment-friendly; and the prepared composite hydrogel has high mechanical strength, high porosity, good connectivity of the interiors of pores and good biocompatibility, and is applicable as a cartilage repairing scaffold to the field of cartilage-repairing tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of textile materials, and in particular relates to a silk protein / bacterial cellulose composite hydrogel and a preparation method and application thereof. Background technique [0002] With the aging of human society and the continuous increase of high-energy and high-speed trauma, the problems of cartilage tissue defect and cartilage tissue degeneration have become increasingly prominent. In recent years, using the basic principles and methods of tissue engineering, cells or growth factors are planted in biodegradable and biocompatible biomaterials to form composites and implanted in cartilage defects to repair, maintain and improve tissue Features. However, the tissue engineered cartilage constructed in the past has problems such as insufficient mechanical strength, "hollow" large cartilage, calcification of the repair tissue matrix, and poor long-term efficacy, making it difficult to obtain ideal clinica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/26A61L27/50
CPCA61L27/26A61L27/50A61L27/52A61L2430/06C08L89/00C08L1/02
Inventor 王曙东马倩王可胡锋
Owner 江苏臻中滤料科技有限公司
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